Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACCQ18_RS07235 Genome accession   NZ_CP167830
Coordinates   1623095..1624225 (+) Length   376 a.a.
NCBI ID   WP_003489921.1    Uniprot ID   A0AAI7ZGP8
Organism   Xanthomonas axonopodis pv. desmodiilaxiflori strain NCPPB 3086     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1618095..1629225
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACCQ18_RS07210 (ACCQ18_07205) - 1618205..1618846 (-) 642 WP_033479080.1 M23 family metallopeptidase -
  ACCQ18_RS07215 (ACCQ18_07210) - 1618953..1619378 (-) 426 WP_003489904.1 HU family DNA-binding protein -
  ACCQ18_RS07220 (ACCQ18_07215) proC 1619932..1620786 (-) 855 WP_003489908.1 pyrroline-5-carboxylate reductase -
  ACCQ18_RS07225 (ACCQ18_07220) - 1620830..1621522 (-) 693 WP_005913298.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACCQ18_RS07230 (ACCQ18_07225) - 1621934..1622972 (+) 1039 Protein_1417 type IV pilus twitching motility protein PilT -
  ACCQ18_RS07235 (ACCQ18_07230) pilU 1623095..1624225 (+) 1131 WP_003489921.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACCQ18_RS07240 (ACCQ18_07235) - 1624719..1625393 (+) 675 WP_033479081.1 YitT family protein -
  ACCQ18_RS07245 (ACCQ18_07240) - 1625568..1626494 (-) 927 WP_172107359.1 DUF72 domain-containing protein -
  ACCQ18_RS07250 (ACCQ18_07245) - 1626502..1627076 (-) 575 Protein_1421 DNA-3-methyladenine glycosylase I -
  ACCQ18_RS07255 (ACCQ18_07250) - 1627365..1627931 (+) 567 WP_005913286.1 YqgE/AlgH family protein -
  ACCQ18_RS07260 (ACCQ18_07255) ruvX 1627924..1628391 (+) 468 WP_003489932.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42044.26 Da        Isoelectric Point: 6.8286

>NTDB_id=1039670 ACCQ18_RS07235 WP_003489921.1 1623095..1624225(+) (pilU) [Xanthomonas axonopodis pv. desmodiilaxiflori strain NCPPB 3086]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELSLPPVIKTLSMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRSRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGLDGVEISEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1039670 ACCQ18_RS07235 WP_003489921.1 1623095..1624225(+) (pilU) [Xanthomonas axonopodis pv. desmodiilaxiflori strain NCPPB 3086]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCATCAGAAGGCGTCGGACCTGTTCATCACCTCGGGGAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCAGCCCGATTACCCAGACCCCGCTGACCGCGCAGCAGAGTCGCGATCTGG
TGTTGAACGTGATGACGCCGTCGCAGCGCGAGGAATTCGAAAAGACCCACGAGTGCAATTTCGCCATCGGCGTGTCCGGG
GTCGGGCGTTTTCGTGTCAGCTGCTTCTACCAGCGTAATCAGGTGGGCATGGTGCTGCGCCGGATCGAAACGCGCATCCC
CACCGTGGACGAGCTGAGCCTGCCGCCGGTGATAAAGACGCTGTCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGTACCGGTAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAACTCGACCGGGCACATCATCACC
ATCGAAGACCCGATCGAATTCGTGCACAAGCACGAGGGTTGCATCATCACCCAGCGCGAGGTCGGCATCGACACCGACAG
CTGGGAGAACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGACCATGCGATCGCATTCGCCGAAACCGGCCACCTGGTGCTGTGCACGCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCCGAAGATCGCCGCAACCAGCTGTTGATGGATCTATCGCTGAATCTCAAGGGTGTGGT
GGCGCAGCAGCTGATTCCGACCCCGGACGGGCGCAGCCGTCGCGTGGCGATGGAAATCATGTTGGGCACGCCGCTGGTGC
AGGACTACATACGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GATCAGAGCCTGTTCGAGCTCTACCAGGCCGGCGAAATCAGCTACGAAGACGCGTTGCGCTACGCCGACTCGCAGAACGA
GGTGCGCTTGCGCATCAAGCTGTCGCAGGGCGGCGATGCCAAGACCTTGTCGCAGGGTCTGGATGGCGTGGAAATTTCCG
AAGTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.757

97.606

0.652
  pilU Acinetobacter baylyi ADP1

64.146

94.947

0.609
  pilU Vibrio cholerae strain A1552

52.809

94.681

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.457

94.947

0.394
  pilT Pseudomonas aeruginosa PAK

40.896

89.096

0.364
  pilT Acinetobacter nosocomialis M2

39.826

91.489

0.364
  pilT Acinetobacter baumannii D1279779

39.826

91.489

0.364
  pilT Acinetobacter baumannii strain A118

39.826

91.489

0.364

Multiple sequence alignment