Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABQY74_RS15350 Genome accession   NZ_CP167821
Coordinates   3588335..3590068 (-) Length   577 a.a.
NCBI ID   WP_349792116.1    Uniprot ID   -
Organism   Xanthomonas sp. WHRI 7064     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3583335..3595068
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABQY74_RS15340 (ABQY74_015340) - 3584675..3586288 (+) 1614 WP_016901263.1 PAS domain-containing sensor histidine kinase -
  ABQY74_RS15345 (ABQY74_015345) pilR 3586481..3587875 (+) 1395 WP_016901261.1 sigma-54 dependent transcriptional regulator Regulator
  ABQY74_RS15350 (ABQY74_015350) pilB 3588335..3590068 (-) 1734 WP_349792116.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABQY74_RS15355 (ABQY74_015355) - 3590253..3590657 (-) 405 WP_043088084.1 pilin -
  ABQY74_RS15360 (ABQY74_015360) pilC 3591035..3592294 (+) 1260 WP_349792119.1 type II secretion system F family protein Machinery gene
  ABQY74_RS15365 (ABQY74_015365) - 3592301..3593164 (+) 864 WP_016849610.1 A24 family peptidase -
  ABQY74_RS15370 (ABQY74_015370) coaE 3593178..3593801 (+) 624 WP_349792121.1 dephospho-CoA kinase -
  ABQY74_RS15375 (ABQY74_015375) - 3593922..3594056 (+) 135 WP_372470099.1 SymE family type I addiction module toxin -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62551.73 Da        Isoelectric Point: 5.6006

>NTDB_id=1039588 ABQY74_RS15350 WP_349792116.1 3588335..3590068(-) (pilB) [Xanthomonas sp. WHRI 7064]
MNVVIANLVGITGIARRLVQDGALDESAARLAMDQASAAKVPLPQWFAEKKLVTASQLAAANAVEFGMPLMDVSVFDANQ
NAVKLVSEELLQKYQVLPLFKRGNRLFVGVSSPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNASLGSSLGD
DDEGMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAPV
KLSQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIEKLGYEADQQKL
FLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
IIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLPE
HALLAEGFTPAQIAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQSIGIRDLRQSALVK
AAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=1039588 ABQY74_RS15350 WP_349792116.1 3588335..3590068(-) (pilB) [Xanthomonas sp. WHRI 7064]
ATGAACGTAGTCATTGCCAATCTGGTCGGCATTACCGGCATTGCTCGTCGGCTTGTCCAAGACGGGGCTTTAGATGAGTC
AGCGGCAAGGCTGGCGATGGATCAGGCTAGCGCTGCCAAGGTGCCTCTGCCCCAATGGTTTGCTGAGAAAAAATTGGTAA
CCGCGTCTCAACTTGCCGCCGCTAACGCGGTCGAATTCGGTATGCCACTGATGGACGTGTCGGTCTTCGACGCCAATCAG
AACGCGGTCAAACTGGTCAGTGAGGAGTTGCTCCAGAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTTGTT
CGTGGGGGTGAGCAGCCCGACCCAGACCAGGGCGCTGGACGACATCAAGTTTCATACCAATTTGGTGGTCGAGCCGATCC
TGGTTGACGAGGACCAGATCCGTCGTACCTTGGAGCAGTGGCAGGCCAGCAACGCATCGTTGGGCTCGTCGCTCGGGGAC
GATGATGAGGGGATGGGCGACCTGGACGTGTCGGCCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGATGC
CAAGGGCGACGACACGCCGGTAGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCCGACATCC
ATTTCGAGCCCTATGAAGACGACTACCGGGTGCGGCTGCGGATCGATGGACTGCTGAAGAACGTGGCCAAGGCGCCGGTG
AAGTTGAGCCAGCGTATCGCGGCACGCCTGAAGGTGATGTCGCAGCTGGATATTGCCGAGAAGCGGGTGCCGCAGGACGG
GCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGCACCTTGCCGACATTGTTCGGCGAAAAGG
TAGTGTTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGAGAAGCTGGGCTACGAGGCGGACCAGCAGAAGCTG
TTCCTGGAGGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGCTGTA
CACTGCACTGGGCATCCTCAACGACGAGACGCGCAACATCTCCACCGCCGAGGACCCGGTGGAAATCCGGTTGCCCGGCG
TCAATCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCCGCGCTACGCTCGTTTCTGCGCCAGGATCCGGAC
ATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGTCATATGGTGCT
GTCCACGCTGCATACCAACGATGCGCCGCAGACCATCGCGCGTTTGATGAACATGGGCATCGCGCCGTACAACATCACCT
CGTCGGTGACCCTGGTGATCGCGCAGCGTCTGGCGCGGCGCCTGTGCAACAACTGCAAGCGCAAATCGACCTTGCCCGAG
CATGCGTTGCTGGCCGAAGGCTTCACGCCGGCACAGATCGCTGCCGGTATCGAGTTGTATGAGGCGGTCGGTTGCGACGA
GTGCACCGAAGGCTACAAGGGCCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGGCGCGATCGTGCTGG
AAGGCGGTAACGCGATGCAGATTGCCGAGGCGGCACAGAGTATCGGCATCCGCGACCTGCGCCAGTCGGCGTTGGTCAAG
GCCGCGCACGGGGTGACCAGCTTGGCCGAGATCAACCGGGTGACGAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.282

98.44

0.544
  pilB Acinetobacter baylyi ADP1

55.106

98.44

0.542
  pilB Legionella pneumophila strain ERS1305867

52.548

98.614

0.518
  pilB Vibrio cholerae strain A1552

48.966

100

0.492
  pilF Neisseria gonorrhoeae MS11

48.768

98.44

0.48
  pilB Vibrio parahaemolyticus RIMD 2210633

46.964

97.054

0.456
  pilB Vibrio campbellii strain DS40M4

46.046

98.614

0.454
  pilB/pilB1 Synechocystis sp. PCC 6803

36.125

100

0.381
  pilF Thermus thermophilus HB27

40.192

90.121

0.362

Multiple sequence alignment