Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACCQ28_RS14695 Genome accession   NZ_CP167233
Coordinates   3351306..3352436 (-) Length   376 a.a.
NCBI ID   WP_003489921.1    Uniprot ID   A0AAI7ZGP8
Organism   Xanthomonas axonopodis pv. tamarindi strain NCPPB 584     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3346306..3357436
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACCQ28_RS14665 (ACCQ28_14665) ruvX 3346815..3347282 (-) 468 WP_008571823.1 Holliday junction resolvase RuvX -
  ACCQ28_RS14670 (ACCQ28_14670) - 3347275..3347841 (-) 567 WP_003489930.1 YqgE/AlgH family protein -
  ACCQ28_RS14675 (ACCQ28_14675) - 3348127..3348699 (+) 573 WP_372183538.1 DNA-3-methyladenine glycosylase I -
  ACCQ28_RS14680 (ACCQ28_14680) - 3348707..3349633 (+) 927 WP_372183539.1 DUF72 domain-containing protein -
  ACCQ28_RS14685 (ACCQ28_14685) - 3349925..3350176 (+) 252 WP_162010796.1 hypothetical protein -
  ACCQ28_RS14690 (ACCQ28_14690) - 3350212..3351216 (+) 1005 WP_005926112.1 pectate lyase -
  ACCQ28_RS14695 (ACCQ28_14695) pilU 3351306..3352436 (-) 1131 WP_003489921.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACCQ28_RS14700 (ACCQ28_14700) pilT 3352549..3353586 (-) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  ACCQ28_RS14705 (ACCQ28_14705) - 3354004..3354696 (+) 693 WP_372183540.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACCQ28_RS14710 (ACCQ28_14710) proC 3354740..3355594 (+) 855 WP_033478563.1 pyrroline-5-carboxylate reductase -
  ACCQ28_RS14715 (ACCQ28_14715) - 3356154..3356579 (+) 426 WP_008571830.1 HU family DNA-binding protein -
  ACCQ28_RS14720 (ACCQ28_14720) - 3356686..3357327 (+) 642 WP_057680719.1 M23 family metallopeptidase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42044.26 Da        Isoelectric Point: 6.8286

>NTDB_id=1039053 ACCQ28_RS14695 WP_003489921.1 3351306..3352436(-) (pilU) [Xanthomonas axonopodis pv. tamarindi strain NCPPB 584]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELSLPPVIKTLSMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRSRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGLDGVEISEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1039053 ACCQ28_RS14695 WP_003489921.1 3351306..3352436(-) (pilU) [Xanthomonas axonopodis pv. tamarindi strain NCPPB 584]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCATCAGAAGGCCTCGGACCTGTTCATCACCTCGGGCAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCAGCCCGATTACCCAGACCCCGCTGACTGCGCAGCAGAGCCGCGACCTGG
TGTTGAACGTGATGACGCCGTCGCAGCGCGAGGAATTCGAAAAGACCCACGAGTGCAATTTCGCCATCGGCGTATCCGGG
GTCGGGCGTTTCCGTGTGAGCTGCTTCTATCAGCGTAATCAGGTGGGCATGGTGCTGCGCCGGATCGAAACGCGCATCCC
CACCGTGGACGAGCTGAGCTTGCCGCCGGTGATCAAGACGCTGTCGATGACCAAGCGCGGCATCATCATCTTTGTCGGCG
CCACCGGAACCGGTAAATCGACTTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAATTCCACCGGGCACATCATCACC
ATCGAGGACCCGATCGAATTCGTGCACAAGCACGAGGGTTGCATCATCACCCAGCGCGAGGTCGGCATCGACACCGACAG
CTGGGAGAACGCGTTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGACCATGCGATCGCATTCGCCGAAACCGGCCATCTGGTGCTGTGCACGCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCCGAAGATCGCCGCAACCAGCTGTTGATGGACCTGTCGCTCAACCTCAAGGGCGTGGT
CGCGCAGCAGCTGATTCCGACCCCGGACGGCCGCAGCCGTCGCGTGGCGATGGAAATCATGTTGGGCACGCCGCTGGTGC
AGGACTACATACGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GACCAGAGCCTGTTCGAGCTCTACCAGGCCGGCGAAATCAGTTACGAAGACGCGTTGCGCTACGCCGACTCGCAGAACGA
GGTCCGCCTGCGCATCAAGCTCTCGCAGGGCGGCGATGCCAAGACCTTGTCGCAGGGCCTGGACGGCGTGGAAATTTCCG
AAGTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.757

97.606

0.652
  pilU Acinetobacter baylyi ADP1

64.146

94.947

0.609
  pilU Vibrio cholerae strain A1552

52.809

94.681

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.457

94.947

0.394
  pilT Pseudomonas aeruginosa PAK

40.896

89.096

0.364
  pilT Acinetobacter nosocomialis M2

39.826

91.489

0.364
  pilT Acinetobacter baumannii D1279779

39.826

91.489

0.364
  pilT Acinetobacter baumannii strain A118

39.826

91.489

0.364

Multiple sequence alignment