Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACCQ23_RS17525 Genome accession   NZ_CP167230
Coordinates   3985173..3986909 (-) Length   578 a.a.
NCBI ID   WP_372177099.1    Uniprot ID   -
Organism   Xanthomonas axonopodis pv. phyllanthi strain NCPPB 2066     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3980173..3991909
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACCQ23_RS17510 (ACCQ23_17510) sucC 3980214..3981383 (-) 1170 WP_007965356.1 ADP-forming succinate--CoA ligase subunit beta -
  ACCQ23_RS17515 (ACCQ23_17515) - 3981615..3983228 (+) 1614 WP_011348336.1 PAS domain-containing sensor histidine kinase -
  ACCQ23_RS17520 (ACCQ23_17520) pilR 3983497..3984951 (+) 1455 WP_372177098.1 sigma-54-dependent transcriptional regulator Regulator
  ACCQ23_RS17525 (ACCQ23_17525) pilB 3985173..3986909 (-) 1737 WP_372177099.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACCQ23_RS17530 (ACCQ23_17530) pilA 3986951..3987364 (-) 414 WP_372177100.1 pilin Machinery gene
  ACCQ23_RS17535 (ACCQ23_17535) comP 3987461..3987889 (-) 429 WP_005921364.1 pilin Machinery gene
  ACCQ23_RS17540 (ACCQ23_17540) pilC 3988237..3989496 (+) 1260 WP_372177101.1 type II secretion system F family protein Machinery gene
  ACCQ23_RS17545 (ACCQ23_17545) - 3989503..3990366 (+) 864 WP_184419734.1 A24 family peptidase -
  ACCQ23_RS17550 (ACCQ23_17550) coaE 3990380..3990991 (+) 612 WP_184419733.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62692.78 Da        Isoelectric Point: 5.5986

>NTDB_id=1039021 ACCQ23_RS17525 WP_372177099.1 3985173..3986909(-) (pilB) [Xanthomonas axonopodis pv. phyllanthi strain NCPPB 2066]
MNVAIGSNLVGITGIARRLVQDGALDEASARAAMDHATQAKVPLPQWFAEKKLVTAAQLAAANAVEFGMPLLDVSAFDAN
QNAVKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNASLGSSLG
NDDEEMGDLDVSTGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
DNALLAEGFTPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALM
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=1039021 ACCQ23_RS17525 WP_372177099.1 3985173..3986909(-) (pilB) [Xanthomonas axonopodis pv. phyllanthi strain NCPPB 2066]
ATGAACGTTGCAATCGGCTCAAATCTAGTTGGAATTACTGGTATTGCGCGGCGCCTTGTTCAGGATGGTGCGCTTGATGA
GGCGAGTGCGCGTGCGGCAATGGATCACGCAACGCAGGCCAAAGTTCCTTTGCCGCAATGGTTCGCCGAAAAAAAGCTTG
TAACAGCCGCGCAACTCGCGGCCGCCAACGCAGTCGAGTTTGGCATGCCGTTGCTGGACGTGTCGGCATTCGATGCTAAC
CAGAACGCGGTCAAGCTGGTCAGCGAGGAGTTGCTCCAGAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGCAATCGGTT
GTTTGTGGGAGTGAGCAACCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTCCACACCAATCTGGTGGTCGAGCCGA
TCTTGGTCGACGAGGACCAGATTCGTCGTACCTTGGAACAGTGGCAGGCCAGCAACGCGTCGTTGGGTTCGTCGCTTGGC
AACGACGACGAGGAGATGGGGGATCTGGACGTCTCCACCGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCAGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGCTTGCGCATCGACGGCTTGTTGAAGAACGTGGCGAAGGCGCCG
GTGAAGCTGAACCAGCGCATAGCAGCGCGCTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTTCGTGTCAGCACCTTGCCGACCCTGTTCGGTGAAA
AAGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTATGAGGCGGACCAGCAGAAG
CTGTTCCTGGAGGCGATCCACAAGCCGTACGGGATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGCT
GTACACCGCGCTGGGAATTCTCAACGACGAGACACGCAATATCTCCACCGCGGAGGACCCGGTCGAAATCCGCTTGCCTG
GCGTCAATCAGGTACAGCAGAACAACAAGCGTGGCATGACCTTTGCAGCAGCGCTGCGCTCGTTCCTGCGTCAGGACCCT
GACATCATCATGGTTGGCGAAATCCGTGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGTCACATGGT
GTTGTCGACGTTGCACACCAACGATGCGCCGCAGACCATCGCACGCTTGATGAACATGGGCATCGCGCCCTACAACATTA
CCTCGTCGGTGACCTTGGTGATCGCGCAGCGTCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCT
GACAACGCCTTGCTGGCCGAAGGATTCACGCCCGCACAGCTTGCTGCCGGGATCGAGCTGTATGAGGCGGTCGGTTGCGA
CGAGTGCACCGAAGGCTACAAGGGGCGTACCGGTATCTACCAGGTCATGCCGATGACCGACGAGATCGGCGCGATCGTGC
TGGAGGGCGGCAATGCGATGCAGATTGCCGAGGCCGCGCAGGCGATCGGTATCCGCGATTTGCGGCAGTCGGCCTTGATG
AAGGCTGCCCACGGGGTGACCAGCCTGGCCGAGATCAATCGGGTGACGAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.162

98.27

0.552
  pilB Acinetobacter baylyi ADP1

55.634

98.27

0.547
  pilB Legionella pneumophila strain ERS1305867

51.211

100

0.512
  pilB Vibrio cholerae strain A1552

48.962

100

0.49
  pilF Neisseria gonorrhoeae MS11

49.296

98.27

0.484
  pilB Vibrio parahaemolyticus RIMD 2210633

45.567

97.578

0.445
  pilB Vibrio campbellii strain DS40M4

44.815

98.443

0.441
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.885

90.657

0.362

Multiple sequence alignment