Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACCP96_RS14355 Genome accession   NZ_CP167226
Coordinates   3421348..3422478 (-) Length   376 a.a.
NCBI ID   WP_003489921.1    Uniprot ID   A0AAI7ZGP8
Organism   Xanthomonas campestris pv. fici strain NCPPB 3886     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3416348..3427478
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACCP96_RS14325 (ACCP96_14325) ruvX 3416866..3417333 (-) 468 WP_372156869.1 Holliday junction resolvase RuvX -
  ACCP96_RS14330 (ACCP96_14330) - 3417326..3417892 (-) 567 WP_003489930.1 YqgE/AlgH family protein -
  ACCP96_RS14335 (ACCP96_14335) - 3418169..3418741 (+) 573 WP_014090665.1 DNA-3-methyladenine glycosylase I -
  ACCP96_RS14340 (ACCP96_14340) - 3418749..3419675 (+) 927 WP_218559508.1 DUF72 domain-containing protein -
  ACCP96_RS14345 (ACCP96_14345) - 3419967..3420218 (+) 252 WP_162010796.1 hypothetical protein -
  ACCP96_RS14350 (ACCP96_14350) - 3420254..3421258 (+) 1005 WP_218559507.1 pectate lyase -
  ACCP96_RS14355 (ACCP96_14355) pilU 3421348..3422478 (-) 1131 WP_003489921.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACCP96_RS14360 (ACCP96_14360) pilT 3422591..3423628 (-) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  ACCP96_RS14365 (ACCP96_14365) - 3424040..3424732 (+) 693 WP_372156871.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACCP96_RS14370 (ACCP96_14370) proC 3424776..3425630 (+) 855 WP_011348110.1 pyrroline-5-carboxylate reductase -
  ACCP96_RS14375 (ACCP96_14375) - 3426190..3426615 (+) 426 WP_008571830.1 HU family DNA-binding protein -
  ACCP96_RS14380 (ACCP96_14380) - 3426722..3427363 (+) 642 WP_372156873.1 M23 family metallopeptidase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42044.26 Da        Isoelectric Point: 6.8286

>NTDB_id=1038986 ACCP96_RS14355 WP_003489921.1 3421348..3422478(-) (pilU) [Xanthomonas campestris pv. fici strain NCPPB 3886]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELSLPPVIKTLSMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRSRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGLDGVEISEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1038986 ACCP96_RS14355 WP_003489921.1 3421348..3422478(-) (pilU) [Xanthomonas campestris pv. fici strain NCPPB 3886]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCATCAGAAGGCGTCGGACCTGTTCATCACCTCGGGGAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCAGCCCGATTACCCAGACCCCGCTGACCGCGCAGCAGAGTCGCGATCTGG
TGTTGAACGTGATGACGCCGTCGCAGCGCGAGGAATTCGAAAAGACCCACGAGTGCAATTTCGCCATCGGCGTGTCCGGG
GTCGGGCGTTTTCGTGTCAGCTGCTTCTACCAGCGTAATCAGGTGGGCATGGTGCTGCGCCGGATCGAAACGCGCATCCC
CACCGTGGACGAGCTGAGCCTGCCGCCGGTGATCAAGACGCTGTCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGGACCGGTAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAACTCGACCGGGCACATCATCACC
ATCGAAGATCCGATCGAATTCGTGCACAAGCACGAGGGTTGCATCATCACCCAGCGCGAGGTCGGCATCGACACCGACAG
CTGGGAGAACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGACCATGCGATCGCCTTCGCCGAAACCGGCCATCTGGTGCTGTGCACGCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCCGAAGATCGCCGCAACCAGCTGTTGATGGACCTGTCGCTCAACCTCAAGGGCGTGGT
CGCGCAGCAGCTGATTCCGACCCCGGACGGCCGCAGCCGTCGCGTGGCGATGGAAATCATGTTGGGCACGCCGCTGGTGC
AGGACTACATACGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTTGGCATGCGCACCTTC
GACCAGAGCCTGTTCGAGCTCTACCAGGCCGGCGAAATCAGTTACGAAGATGCGCTGCGCTACGCCGACTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTCTCGCAGGGCGGCGATGCCAAGACCTTGTCGCAGGGCCTGGACGGCGTGGAAATTTCCG
AAGTGCGCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.757

97.606

0.652
  pilU Acinetobacter baylyi ADP1

64.146

94.947

0.609
  pilU Vibrio cholerae strain A1552

52.809

94.681

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.457

94.947

0.394
  pilT Pseudomonas aeruginosa PAK

40.896

89.096

0.364
  pilT Acinetobacter nosocomialis M2

39.826

91.489

0.364
  pilT Acinetobacter baumannii D1279779

39.826

91.489

0.364
  pilT Acinetobacter baumannii strain A118

39.826

91.489

0.364

Multiple sequence alignment