Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACCP93_RS14590 Genome accession   NZ_CP167216
Coordinates   3379284..3381020 (-) Length   578 a.a.
NCBI ID   WP_218558981.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. fici strain NCPPB 2372     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3374284..3386020
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACCP93_RS14575 (ACCP93_14575) - 3375337..3376950 (+) 1614 WP_218558976.1 PAS domain-containing sensor histidine kinase -
  ACCP93_RS14580 (ACCP93_14580) pilR 3377219..3378673 (+) 1455 WP_218558977.1 sigma-54 dependent transcriptional regulator Regulator
  ACCP93_RS14585 (ACCP93_14585) pilB 3378887..3379006 (-) 120 WP_136732873.1 pilus assembly protein Machinery gene
  ACCP93_RS14590 (ACCP93_14590) pilB 3379284..3381020 (-) 1737 WP_218558981.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACCP93_RS14595 (ACCP93_14595) pilA2 3381062..3381475 (-) 414 WP_005921365.1 pilin Machinery gene
  ACCP93_RS14600 (ACCP93_14600) comP 3381572..3382000 (-) 429 WP_005921364.1 pilin Machinery gene
  ACCP93_RS14605 (ACCP93_14605) pilC 3382345..3383604 (+) 1260 WP_033836749.1 type II secretion system F family protein Machinery gene
  ACCP93_RS14610 (ACCP93_14610) - 3383611..3384474 (+) 864 WP_003491180.1 A24 family peptidase -
  ACCP93_RS14615 (ACCP93_14615) coaE 3384488..3385096 (+) 609 WP_078517255.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62665.78 Da        Isoelectric Point: 5.9134

>NTDB_id=1038911 ACCP93_RS14590 WP_218558981.1 3379284..3381020(-) (pilB) [Xanthomonas campestris pv. fici strain NCPPB 2372]
MNVAIGSNLVGITGIARRLVQDGALDEASARAAMDHATQAKVPLPQWFAEKKLVTAAQLAAANAVEFGMPLMDVSAFDAN
QNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEAILVDEDQIRRTLEQWQASNASLGSSLG
NDDEEMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNHCKRKSTLP
ENALLAEGFTPAQIAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALM
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=1038911 ACCP93_RS14590 WP_218558981.1 3379284..3381020(-) (pilB) [Xanthomonas campestris pv. fici strain NCPPB 2372]
ATGAACGTTGCAATCGGCTCAAATCTAGTTGGAATTACTGGTATTGCGCGGCGCCTTGTTCAGGATGGTGCGCTTGATGA
GGCGAGCGCGCGTGCGGCAATGGATCACGCAACCCAGGCCAAAGTTCCTTTACCGCAATGGTTCGCCGAAAAGAAGCTTG
TAACAGCCGCACAACTCGCGGCCGCCAACGCAGTCGAGTTTGGCATGCCTTTGATGGATGTATCGGCGTTCGATGCCAAC
CAGAACGCGGTCAAGCTGGTCAGCGAGGAGTTGCTCCAGAAACACCAAGTGTTGCCGCTGTTCAAGCGCGGTAACCGGTT
GTTCGTGGGGGTGAGCAACCCGACACAGACTCGCGCGCTGGACGACATCAAGTTCCATACGAACTTGGTGGTCGAGGCAA
TCCTCGTCGACGAAGATCAGATCCGCCGGACGCTCGAGCAGTGGCAGGCCAGCAATGCGTCGTTGGGTTCTTCGCTTGGC
AACGACGACGAGGAAATGGGGGATCTGGACGTCTCGGCCGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCAGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGCTTGCGCATCGACGGCTTGTTGAAGAACGTGGCGAAGGCGCCG
GTGAAGCTGAACCAGCGCATCGCAGCGCGCTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTTCGTGTCAGCACCTTGCCGACCCTGTTCGGTGAAA
AAGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTATGAGGCGGACCAGCAGAAG
CTGTTCCTGGAGGCGATCCACAAGCCGTACGGGATGGTGCTGGTGACCGGGCCGACTGGTTCGGGCAAGACGGTGTCGCT
GTATACGGCGCTGGGCATCCTCAACGACGAGACGCGCAACATCTCCACCGCCGAGGATCCGGTCGAAATCCGCTTGCCAG
GCGTCAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCCTTGCGCTCGTTCCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATCGCGATCAAGGCGGCGCAGACCGGCCACATGGT
GCTTTCGACGCTGCATACCAACGATGCGCCGCAGACCATCGCACGTCTGATGAACATGGGCATTGCGCCCTACAACATCA
CTTCGTCGGTGACGTTGGTGATCGCGCAGCGTCTGGCGCGGCGGTTGTGCAACCACTGCAAGCGCAAGTCGACGCTGCCG
GAGAATGCGTTGCTGGCCGAAGGCTTCACGCCGGCGCAGATCGCCGCCGGGATCGAGCTGTACGAGGCGGTCGGTTGCGA
CGAGTGCACCGAAGGCTACAAGGGGCGTACCGGTATCTACCAGGTCATGCCGATGACCGACGAGATCGGCGCGATCGTGC
TGGAGGGCGGCAATGCGATGCAGATTGCCGAGGCCGCGCAGGCGATCGGTATCCGCGATTTGCGGCAGTCGGCCTTGATG
AAGGCTGCCCACGGGGTTACCAGCCTGGCCGAGATCAATCGTGTAACGAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-153)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

98.27

0.55
  pilB Acinetobacter baylyi ADP1

55.106

98.27

0.542
  pilB Legionella pneumophila strain ERS1305867

52.021

98.443

0.512
  pilB Vibrio cholerae strain A1552

49.135

100

0.491
  pilF Neisseria gonorrhoeae MS11

48.944

98.27

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

46.429

96.886

0.45
  pilB Vibrio campbellii strain DS40M4

45.518

98.443

0.448

Multiple sequence alignment