Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AB9L18_RS15605 Genome accession   NZ_CP167120
Coordinates   3426130..3427272 (-) Length   380 a.a.
NCBI ID   WP_375067824.1    Uniprot ID   -
Organism   Stenotrophomonas lactitubi strain RW9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3421130..3432272
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB9L18_RS15580 (AB9L18_15580) - 3421657..3422364 (-) 708 WP_025874035.1 YitT family protein -
  AB9L18_RS15585 (AB9L18_15585) - 3422694..3422906 (-) 213 WP_033830520.1 DUF4287 domain-containing protein -
  AB9L18_RS15590 (AB9L18_15590) - 3423348..3424247 (+) 900 WP_375067820.1 LysR substrate-binding domain-containing protein -
  AB9L18_RS15595 (AB9L18_15595) - 3424297..3425280 (-) 984 WP_100463493.1 aldo/keto reductase -
  AB9L18_RS15600 (AB9L18_15600) - 3425420..3426088 (-) 669 WP_375067822.1 PIN domain-containing protein -
  AB9L18_RS15605 (AB9L18_15605) pilU 3426130..3427272 (-) 1143 WP_375067824.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB9L18_RS15610 (AB9L18_15610) pilT 3427373..3428410 (-) 1038 WP_004147099.1 type IV pilus twitching motility protein PilT Machinery gene
  AB9L18_RS15615 (AB9L18_15615) - 3428498..3429175 (+) 678 WP_205405054.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB9L18_RS15620 (AB9L18_15620) proC 3429192..3430013 (+) 822 WP_346558738.1 pyrroline-5-carboxylate reductase -
  AB9L18_RS15625 (AB9L18_15625) - 3430069..3430944 (-) 876 WP_375067829.1 LysR family transcriptional regulator -
  AB9L18_RS15630 (AB9L18_15630) - 3431043..3431645 (+) 603 WP_100551721.1 short chain dehydrogenase -
  AB9L18_RS15635 (AB9L18_15635) soxR 3431683..3432117 (-) 435 WP_032951587.1 redox-sensitive transcriptional activator SoxR -

Sequence

Protein


Download         Length: 380 a.a.        Molecular weight: 42351.68 Da        Isoelectric Point: 6.7684

>NTDB_id=1038270 AB9L18_RS15605 WP_375067824.1 3426130..3427272(-) (pilU) [Stenotrophomonas lactitubi strain RW9]
MNTTATTIDFTSFLKLMAHQRASDLFITAGMPPAIKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAI
GLSGVGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQNSTG
HIITIEDPIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIGFAETGHLVLCTLHANNA
NQAMDRIVNFFPEDRRNQLLMDLSLNLKGVVAQQLIPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKELMKESVQLG
MKTFDQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEVR

Nucleotide


Download         Length: 1143 bp        

>NTDB_id=1038270 AB9L18_RS15605 WP_375067824.1 3426130..3427272(-) (pilU) [Stenotrophomonas lactitubi strain RW9]
GTGAACACCACCGCGACCACCATCGATTTCACCTCGTTCCTCAAGCTGATGGCGCACCAGCGCGCCTCGGACCTGTTCAT
CACCGCTGGCATGCCGCCGGCGATCAAGGTCAACGGCAAGATCTCGCCGATCACCCAGACTCCGCTGACGCCGCAGCAGA
GCCGCGACCTGGTGCTGAACGTGATGACGCCGGCGCAGCGCGAGGAATTCGAAAAGACCCACGAATGCAACTTCGCCATC
GGCCTGTCCGGTGTCGGCCGCTTCCGTGTGAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGTCGCATCGA
GACGCGCATCCCGACCGTGGAAGAGCTGAGCCTGCCGCCGATCATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCC
TGTTCGTCGGCGCTACCGGTACCGGTAAATCCACGTCGCTGGCCGCGATGATCGGCTACCGCAACCAGAATTCGACCGGG
CACATCATCACCATCGAAGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTCGGCAT
CGATACCGACAGCTGGGAAGCCGCGCTGAAGAACACCCTGCGCCAGGCCCCGGACGTGATCATGATCGGCGAGGTGCGTA
CCCGCGAGGGCATGGACCACGCCATCGGCTTCGCTGAAACCGGTCACCTGGTGCTGTGCACCCTGCACGCCAACAATGCC
AACCAGGCGATGGACCGCATCGTCAACTTCTTCCCGGAAGATCGCCGCAACCAGCTGCTGATGGATCTGTCGCTGAACCT
CAAGGGCGTGGTCGCGCAGCAGTTGATTCCGTCGCCGGATGGCCGCTCGCGCAAGGTCGCGATGGAGATCCTGCTGGGCA
CGCCGCTGGTGCAGGACTACATCCGCGACGGCGAGATCCACAAGCTGAAGGAGCTGATGAAGGAATCGGTCCAGTTGGGC
ATGAAGACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCCGGTGAGATCAGCTACGAGGACGCACTGCGCTACGCCGA
TTCGCAGAACGAAGTGCGCCTGCGCATCAAGCTCAGCCAGGGCGGCGATGCCCGCACCCTGTCGCAGGGGCTGGATGGGG
TGGAGATTTCCGAGGTCCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.302

96.579

0.65
  pilU Acinetobacter baylyi ADP1

63.866

93.947

0.6
  pilU Vibrio cholerae strain A1552

53.371

93.684

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.817

93.421

0.4
  pilT Pseudomonas aeruginosa PAK

40.896

88.158

0.361
  pilT Acinetobacter nosocomialis M2

39.826

90.526

0.361
  pilT Acinetobacter baumannii D1279779

39.826

90.526

0.361
  pilT Acinetobacter baumannii strain A118

39.826

90.526

0.361

Multiple sequence alignment