Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AB9L18_RS04405 Genome accession   NZ_CP167120
Coordinates   966079..967806 (+) Length   575 a.a.
NCBI ID   WP_375069533.1    Uniprot ID   -
Organism   Stenotrophomonas lactitubi strain RW9     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 961079..972806
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB9L18_RS04380 (AB9L18_04380) - 961690..962076 (+) 387 WP_375069525.1 hypothetical protein -
  AB9L18_RS04385 (AB9L18_04385) coaE 962129..962740 (-) 612 WP_375069527.1 dephospho-CoA kinase -
  AB9L18_RS04390 (AB9L18_04390) - 962752..963615 (-) 864 WP_025874291.1 A24 family peptidase -
  AB9L18_RS04395 (AB9L18_04395) pilC 963624..964883 (-) 1260 WP_375069529.1 type II secretion system F family protein Machinery gene
  AB9L18_RS04400 (AB9L18_04400) comP 965233..965649 (+) 417 WP_375069531.1 pilin Machinery gene
  AB9L18_RS04405 (AB9L18_04405) pilB 966079..967806 (+) 1728 WP_375069533.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AB9L18_RS04410 (AB9L18_04410) pilR 967873..969246 (-) 1374 WP_100553378.1 sigma-54 dependent transcriptional regulator Regulator
  AB9L18_RS04415 (AB9L18_04415) - 969301..970914 (-) 1614 WP_375069534.1 PAS domain-containing sensor histidine kinase -
  AB9L18_RS04420 (AB9L18_04420) sucC 971292..972461 (+) 1170 WP_025874298.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62202.16 Da        Isoelectric Point: 6.1548

>NTDB_id=1038262 AB9L18_RS04405 WP_375069533.1 966079..967806(+) (pilB) [Stenotrophomonas lactitubi strain RW9]
MNAVTSANLVGITGLARRLVQDGALDEAAARDAMAKATAARQPLPTWFAQNKVVGAAQLAAANSLEFGMPLFDVSTFDSS
QNAMSLVSEELLRKHNVLPLFKRGGKLFVGTSNPTHSLDEIKFHTNLVVEPILVDEDQIRRTLEQWHASHDTMGDALGGD
DDGMANLDVGGGDDEMASTDSGIDAKGDDTPVVKFINKVLIDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPTKL
NQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTRQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKLFL
EAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDII
MVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCSNCKRPTDLPAHA
LLAEGFTQAQLDAGIQLHEAVGCDECTEGYKGRTGLYQVMPMTDEIATIVLAGGNALQIAEAAQAIGVNDLRQSALKKAA
AGVTSLAEINRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=1038262 AB9L18_RS04405 WP_375069533.1 966079..967806(+) (pilB) [Stenotrophomonas lactitubi strain RW9]
ATGAACGCAGTTACCTCCGCCAACCTCGTCGGCATTACCGGTCTGGCCCGCCGCCTGGTTCAGGATGGCGCACTGGATGA
GGCGGCTGCCCGCGACGCCATGGCCAAGGCCACCGCCGCCCGCCAACCGTTGCCAACGTGGTTTGCGCAGAACAAGGTCG
TCGGCGCGGCCCAGTTGGCCGCCGCCAACTCGCTCGAGTTCGGCATGCCCCTGTTCGATGTCTCCACCTTCGACAGCAGC
CAGAACGCGATGAGCCTGGTCAGCGAGGAGCTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAAACGCGGTGGCAAGCT
GTTCGTCGGCACCAGCAACCCCACCCACTCGCTGGATGAGATCAAGTTCCACACCAATCTGGTGGTTGAACCGATCCTGG
TGGACGAGGACCAGATACGCCGCACGTTGGAGCAGTGGCATGCCAGCCACGACACCATGGGCGATGCGCTGGGTGGTGAC
GATGACGGCATGGCCAACCTCGACGTTGGCGGTGGCGACGATGAGATGGCCAGCACCGACAGCGGCATCGACGCCAAGGG
CGACGACACCCCGGTGGTCAAATTCATCAACAAGGTGCTGATCGACGCCATCCGCAAGGGCGCGTCGGACATCCACTTCG
AGCCCTACGAAGACGACTATCGCGTGCGCCTGCGCATTGACGGCCTGCTGAAGATGGTCGCGCGCGCACCGACCAAGCTC
AACCAGCGCATCGCTGCGCGTCTGAAAGTGATGGCGCAGCTGGATATCGCGGAAAAGCGCGTGCCGCAGGATGGTCGCAT
CAAGCTCAACCTGTCCAAGACCCGGCAGATCGACTTCCGTGTCAGCACGTTGCCGACGCTGTTCGGCGAGAAGGTGGTGC
TGCGTATCCTCGATGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGTTACGAGCCGGATCAGCAGAAGTTGTTCCTG
GAAGCGATCCACAAACCGTATGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCTGTATACCGC
GCTGGGCATCCTCAACGACGAGACCCGCAACATTTCCACCGCCGAAGACCCGGTGGAAATCCGCCTGCCCGGTGTCAACC
AGGTGCAGCAGAACAACAAGCGAGGCATGACCTTCGCCGCAGCCCTGCGCTCGTTCCTGCGACAGGATCCGGACATCATC
ATGGTCGGCGAAATCCGCGACCTGGAAACCGCGGAAATTGCGATCAAGGCCGCACAGACCGGTCACATGGTGCTGTCCAC
GCTGCATACCAACGACGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCGCCGTACAACATCACCAGCTCGG
TGACGCTGGTGATCGCCCAGCGCTTGGCGCGCCGCCTGTGTTCGAACTGCAAGCGGCCGACAGACCTGCCCGCGCATGCG
CTGCTGGCAGAAGGCTTCACCCAGGCGCAGCTGGATGCGGGCATCCAGCTGCACGAAGCGGTCGGCTGCGATGAATGCAC
CGAGGGCTACAAGGGCCGTACCGGTCTCTACCAGGTGATGCCGATGACCGACGAGATCGCGACCATCGTGCTGGCGGGCG
GCAACGCGCTGCAGATTGCCGAGGCTGCGCAGGCGATCGGTGTGAACGACCTGCGGCAGTCGGCGTTGAAGAAGGCGGCT
GCAGGTGTGACCAGCCTGGCCGAGATCAACCGCGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-150)

Type II/IV secretion system protein (T2SSE)

(198-465)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

56.437

98.609

0.557
  pilB Acinetobacter baumannii D1279779

56.437

98.609

0.557
  pilB Legionella pneumophila strain ERS1305867

51.585

98.783

0.51
  pilB Vibrio cholerae strain A1552

49.209

98.957

0.487
  pilF Neisseria gonorrhoeae MS11

49.12

98.783

0.485
  pilB Vibrio parahaemolyticus RIMD 2210633

46.41

99.304

0.461
  pilB Vibrio campbellii strain DS40M4

45.105

99.478

0.449

Multiple sequence alignment