Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AB9V63_RS25100 Genome accession   NZ_CP166920
Coordinates   5494048..5496153 (-) Length   701 a.a.
NCBI ID   WP_005765499.1    Uniprot ID   A0AAW4DWS6
Organism   Pseudomonas syringae pv. tomato strain GM 113     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5489048..5501153
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB9V63_RS25085 (AB9V63_25090) - 5490667..5492292 (-) 1626 WP_007245598.1 SPOR domain-containing protein -
  AB9V63_RS25090 (AB9V63_25095) aroB 5492303..5493406 (-) 1104 WP_007245597.1 3-dehydroquinate synthase -
  AB9V63_RS25095 (AB9V63_25100) aroK 5493525..5494043 (-) 519 WP_007245596.1 shikimate kinase AroK -
  AB9V63_RS25100 (AB9V63_25105) pilQ 5494048..5496153 (-) 2106 WP_005765499.1 type IV pilus secretin PilQ Machinery gene
  AB9V63_RS25105 (AB9V63_25110) pilP 5496201..5496728 (-) 528 WP_003378849.1 type 4a pilus biogenesis lipoprotein PilP -
  AB9V63_RS25110 (AB9V63_25115) pilO 5496725..5497348 (-) 624 WP_005765501.1 type 4a pilus biogenesis protein PilO -
  AB9V63_RS25115 (AB9V63_25120) - 5497345..5497971 (-) 627 WP_005765503.1 PilN domain-containing protein -
  AB9V63_RS25120 (AB9V63_25125) pilM 5497971..5499035 (-) 1065 WP_005765505.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 701 a.a.        Molecular weight: 75626.08 Da        Isoelectric Point: 4.9797

>NTDB_id=1036239 AB9V63_RS25100 WP_005765499.1 5494048..5496153(-) (pilQ) [Pseudomonas syringae pv. tomato strain GM 113]
MTRILSIIGVSLCIAMLSPVLQAANLKTLDVAALPGDRIELKLAFDAPVPAPRGYTTAQPARIALDLPGVTSQLETKSRE
LGTGNARSVVVVQAQDRTRVIINLTTLAPYSSRVDGNNLFVVIGQGAAAAQASQPTTAMGAQRVAVPPPAPASARPFIPA
GVKAIRNVDFQRGELGEGNVVIDLSNPSIAPDIQEQGGKIRVDFAKTQLPEPLRVRLDVKDFATPVQFVSASATGDKASI
LIEPSGAFDYSAFQTENKLTISVRPLTNEDLDRRAADKPVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGI
TLRLQNVPWDQALDLVLKTKGLDKRKVGSVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQS
VTSAESKAEERGSITVDDRTNNIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEANVDYNKQLGVRWGGSTNTSRNG
KWTTYGLDDNGDEGGNTNANLTSNIPFVDLGAADATTGIGLGFVTNNTLLDLELSAMEKTGNGEIVSQPKVVTSDKETAK
ILKGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNAVLGVPPIKKNEVNAKVLISDGET
IVIGGVFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSESKSELLVFLTPRIMNNQAISVSR

Nucleotide


Download         Length: 2106 bp        

>NTDB_id=1036239 AB9V63_RS25100 WP_005765499.1 5494048..5496153(-) (pilQ) [Pseudomonas syringae pv. tomato strain GM 113]
ATGACCCGGATTCTCTCGATTATCGGCGTGTCGTTATGCATAGCGATGCTGTCGCCGGTTCTCCAGGCTGCCAACCTCAA
GACCCTGGATGTCGCCGCCCTGCCAGGCGATCGCATTGAGCTCAAGCTTGCCTTTGACGCACCGGTGCCCGCGCCTCGCG
GCTACACCACCGCGCAACCGGCGCGCATCGCTCTGGACCTGCCGGGCGTGACCAGCCAGCTAGAGACCAAAAGCCGTGAG
CTGGGCACCGGCAATGCACGCAGCGTAGTGGTGGTGCAGGCTCAGGATCGAACGCGAGTGATCATCAACCTGACGACGCT
TGCGCCTTACAGCTCTCGCGTTGACGGCAACAATCTGTTTGTGGTCATCGGCCAGGGTGCCGCCGCAGCGCAGGCCTCCC
AGCCGACCACTGCGATGGGCGCGCAACGGGTAGCGGTGCCGCCGCCAGCCCCGGCGTCTGCCAGACCCTTTATACCGGCG
GGCGTCAAAGCCATCCGCAATGTCGATTTTCAGCGTGGTGAGCTGGGCGAAGGCAATGTGGTCATAGACCTGAGTAACCC
GAGCATCGCGCCTGATATTCAGGAACAGGGCGGCAAGATTCGTGTCGACTTCGCCAAGACCCAGTTGCCCGAGCCTCTGC
GTGTGCGGCTGGACGTCAAGGATTTCGCGACCCCTGTGCAGTTCGTCAGTGCGTCGGCCACGGGCGACAAGGCCAGTATT
CTGATCGAGCCGTCGGGTGCCTTCGACTACTCGGCGTTCCAGACCGAAAACAAGCTGACCATCAGTGTGCGTCCGTTGAC
CAACGAAGATCTGGACCGCCGCGCCGCCGACAAGCCGGTGTACACCGGTGAAAAGCTCTCGCTTAACTTCCAGGACATCG
ATGTGCGTTCGGTGTTGCAACTGATCGCCGACTTCACCAACCTCAATCTGGTGGCCAGTGACACGGTTCAGGGCGGCATC
ACCCTGCGCCTGCAGAACGTGCCCTGGGATCAGGCGCTGGACCTGGTGCTGAAAACCAAAGGCCTGGACAAGCGCAAGGT
CGGCAGTGTTCTGCTGGTTGCGCCAGCGGATGAAATTGCCGCCCGCGAACGCCAGGAGCTGGAATCGCTCAAGCAGATTG
CCGAACTGGCACCGCTGCGCCGCGAGCTGTTGCAGGTCAATTACGCCAAGGCCGCCGACATCGCCAAGCTGTTTCAGTCG
GTGACCAGTGCCGAGTCCAAGGCTGAAGAGCGTGGCTCGATCACCGTGGACGACCGCACCAACAACATCATTGCCTACCA
GACTCAGGACCGTCTCGACGAACTGCGTCGCATTGTTTCGCAACTGGATATTCCAGTGCGCCAGGTGATGATCGAGGCGC
GTATCGTCGAGGCCAACGTCGATTACAACAAGCAGTTGGGTGTGCGCTGGGGGGGCTCGACCAATACCAGTCGCAACGGT
AAATGGACCACCTACGGTCTGGACGACAATGGTGATGAGGGCGGTAACACGAATGCAAACCTGACCTCCAACATCCCTTT
TGTTGACCTGGGTGCAGCCGATGCGACCACAGGCATTGGCTTGGGCTTCGTAACCAACAACACGCTGCTGGACCTTGAGC
TGAGCGCGATGGAGAAGACCGGCAACGGTGAAATCGTCTCCCAGCCCAAAGTGGTCACATCCGACAAGGAAACCGCCAAG
ATCCTCAAGGGCACAGAGATTCCGTATCAGGAATCCAGCTCCAGCGGCGCCACCACCGTGAGCTTCAAGGAGGCTTCGCT
GTCGCTTGAAGTCACGCCGCAGATCACGCCGGACAACCGCATCATTATGGAGGTCAAGGTCACCAAGGATGAGCCGGACT
ACCTGAATGCGGTGCTTGGCGTACCGCCCATCAAGAAAAACGAGGTCAACGCCAAAGTGCTGATTTCCGATGGCGAAACC
ATCGTGATCGGTGGTGTGTTCTCCAATACGCAAAGTAAAGTTGTCGATAAAGTGCCATTTCTTGGCGATGTACCGTATCT
TGGCCGGCTTTTCAGGCGCGATGTGGTATCCGAGTCCAAATCCGAGCTGTTGGTATTTTTGACTCCGCGTATCATGAACA
ACCAGGCGATTTCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(383-448)

Secretin and TonB N terminus short domain (STN)

(309-355)

Bacterial type II and III secretion system protein (Secretin)

(536-692)

AMIN domain (AMIN)

(38-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.476

100

0.748
  comQ Acinetobacter baylyi ADP1

41.81

99.287

0.415
  pilQ Acinetobacter baumannii strain A118

38.639

100

0.405
  pilQ Acinetobacter baumannii D1279779

38.639

100

0.405
  pilQ Legionella pneumophila strain Lp02

37.951

98.859

0.375
  pilQ Legionella pneumophila strain ERS1305867

37.951

98.859

0.375

Multiple sequence alignment