Detailed information NTG1036240

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: AB9V63_RS25120
• Genome accession: NZ_CP166920
• Coordinates: 5497971..5499035 (-)
• Length: 354 a.a.
• NCBI ID: WP_005765505.1
• Uniprot ID: A0A3M3ZC03
• Organism: Pseudomonas syringae pv. tomato strain GM 113
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5492971..5504035

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB9V63_RS25095 (AB9V63_25100)	aroK	5493525..5494043 (-)	519	WP_007245596.1	shikimate kinase AroK	-
AB9V63_RS25100 (AB9V63_25105)	pilQ	5494048..5496153 (-)	2106	WP_005765499.1	type IV pilus secretin PilQ	Machinery gene
AB9V63_RS25105 (AB9V63_25110)	pilP	5496201..5496728 (-)	528	WP_003378849.1	type 4a pilus biogenesis lipoprotein PilP	-
AB9V63_RS25110 (AB9V63_25115)	pilO	5496725..5497348 (-)	624	WP_005765501.1	type 4a pilus biogenesis protein PilO	-
AB9V63_RS25115 (AB9V63_25120)	-	5497345..5497971 (-)	627	WP_005765503.1	PilN domain-containing protein	-
AB9V63_RS25120 (AB9V63_25125)	pilM	5497971..5499035 (-)	1065	WP_005765505.1	pilus assembly protein PilM	Machinery gene
AB9V63_RS25125 (AB9V63_25130)	-	5499245..5501686 (+)	2442	WP_371245724.1	penicillin-binding protein 1A	-
AB9V63_RS25130 (AB9V63_25135)	-	5501888..5503156 (-)	1269	WP_003378855.1	malic enzyme-like NAD(P)-binding protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38117.65 Da
• Isoelectric Point: 4.7008

>NTDB_id=1036240 AB9V63_RS25120 WP_005765505.1 5497971..5499035(-) (pilM) [Pseudomonas syringae pv. tomato strain GM 113]
MFELFGKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLIKAKTNVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKDALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=1036240 AB9V63_RS25120 WP_005765505.1 5497971..5499035(-) (pilM) [Pseudomonas syringae pv. tomato strain GM 113]
GTGTTCGAACTCTTTGGTAAGAAGGCCAATACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACTCGTTACAAGGTCGAGTCTTACGCAGTCGAGCCGTTACCGGCCAACGCTGTCGTCGAAAAGAATA
TTGCCGAACTCGAAGGGGTTGGTCAGGCGCTGTCTCGCGTACTGATCAAGGCCAAGACCAACGTCAAGATTGTGGCGGTT
GCCGTGGCGGGTTCCGCGGTGATCACCAAAACCATCGAGATGGACGCCGGTCTCTCCGACGACGACATGGAAAATCAGCT
CAAGCTTGAAGCTGATCAGTACATCCCTTACCCGCTCGAAGAAGTTGCCATCGATTTTGAAGTGCAGGGCTACTCGGTCC
GCAACCCGGAACGTGTCGAAGTCCTGCTGGCGGCCTGCCGCAAGGAAAACGTCGAAGTGCGCGAGGCCGCGCTGGCCCTG
GCCGGGCTGACGGCGCGTGTCGTGGACGTCGAGGCCTACGCGCTGGAGCGTTCATTCGGTCTGCTGGCTGCGCAACTGGG
CAACGGCCATGACGAACTGACCGTTGCCGTGGTTGACATCGGCGCGACCATGACCACCCTCAGCGTGCTGCACCATGGTC
GAATCATCTATACCCGCGAGCAATTGTTCGGCGGTCGCCAGCTGACTGACGAAATTCAGCGCCGCTACGGGCTTTCCATG
GAAGAGGCCGGGCTGGCCAAGAAGCAGGGCGGGCTGCCTGACGATTATGTCAGCGAGGTGCTGGACCCCTTCAAGGACGC
GCTGGTGCAACAGGTTTCCCGATCCCTGCAATTCTTCTTTGCGGCCGGTCAGTACAACTCCGTGGATCACATCATGCTGG
CAGGCGGTACGGCGTCGATTTCAGGGCTGGAGCATTTGATTCAGCGACGTATCGGCACACCGACCATGGTTGCCAACCCG
TTTGCCGACATGGCCTTGAGTTCCAAGGTCAATGCCGGTGCGCTGGCCAGCGATGCACCTGCCCTGATGATCGCGTGCGG
TCTGGCGTTGAGGAGCTTTGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A3M3ZC03

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	56.215	100	0.562
comM	Acinetobacter nosocomialis M2	56.215	100	0.562
comM	Acinetobacter baylyi ADP1	53.672	100	0.537
pilM	Legionella pneumophila strain ERS1305867	46.761	100	0.469