Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACA169_RS16300 Genome accession   NZ_CP166916
Coordinates   3508641..3509771 (-) Length   376 a.a.
NCBI ID   WP_044757021.1    Uniprot ID   -
Organism   Xanthomonas oryzae pv. oryzae strain M10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3503641..3514771
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACA169_RS16260 (ACA169_16260) ruvX 3504209..3504676 (-) 468 WP_011407969.1 Holliday junction resolvase RuvX -
  ACA169_RS16265 (ACA169_16265) - 3504669..3505235 (-) 567 WP_011258225.1 YqgE/AlgH family protein -
  ACA169_RS16270 (ACA169_16270) - 3505311..3506039 (+) 729 WP_240329644.1 hypothetical protein -
  ACA169_RS16275 (ACA169_16275) - 3506068..3506502 (+) 435 WP_027703694.1 hypothetical protein -
  ACA169_RS16280 (ACA169_16280) - 3506499..3507071 (+) 573 WP_011407966.1 DNA-3-methyladenine glycosylase I -
  ACA169_RS16285 (ACA169_16285) - 3507079..3507360 (+) 282 Protein_3120 DUF72 domain-containing protein -
  ACA169_RS16295 (ACA169_16295) - 3507695..3508369 (-) 675 WP_027703695.1 YitT family protein -
  ACA169_RS16300 (ACA169_16300) pilU 3508641..3509771 (-) 1131 WP_044757021.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACA169_RS16305 (ACA169_16305) pilT 3509885..3510922 (-) 1038 WP_011258220.1 type IV pilus twitching motility protein PilT Machinery gene
  ACA169_RS16310 (ACA169_16310) - 3511286..3511978 (+) 693 WP_011407962.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACA169_RS16315 (ACA169_16315) proC 3512022..3512882 (+) 861 WP_011407961.1 pyrroline-5-carboxylate reductase -
  ACA169_RS16320 (ACA169_16320) - 3512953..3514272 (+) 1320 Protein_3126 IS701-like element ISXo15 family transposase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41978.26 Da        Isoelectric Point: 7.1973

>NTDB_id=1036149 ACA169_RS16300 WP_044757021.1 3508641..3509771(-) (pilU) [Xanthomonas oryzae pv. oryzae strain M10]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVNGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELSLPPVIKTLAMTKRGIVIFVGATGAGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPKDRRNQLLMDLSLNLKGVVAQQLIPTPDGRSRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGMDGVEISEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1036149 ACA169_RS16300 WP_044757021.1 3508641..3509771(-) (pilU) [Xanthomonas oryzae pv. oryzae strain M10]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCATCAGAAGGCGTCGGACCTGTTCATCACCTCGGGGAT
GCCGCCGGCGATCAAGGTGAATGGCAAGATCAGCCCGATTACCCAGACGCCGCTGACTGCGCAGCAGAGCCGCGATCTGG
TGTTGAACGTGATGACTCCCTCGCAGCGCGAGGAATTCGAAAAGACCCACGAGTGCAACTTCGCCATCGGCGTTTCAGGG
GTGGGGCGTTTTCGTGTGAGCTGTTTCTACCAGCGTAATCAGGTCGGTATGGTGCTGCGCCGGATCGAAACGCGCATCCC
CACCGTGGACGAGCTGAGCCTGCCTCCGGTGATCAAGACGCTGGCGATGACCAAGCGCGGCATCGTCATCTTCGTCGGCG
CCACCGGTGCCGGTAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAATTCCACCGGCCACATCATCACC
ATCGAAGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGATACCGACAG
CTGGGAAAACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGTACCCGCGAGGGCA
TGGACCACGCGATCGCCTTCGCCGAAACCGGCCACCTGGTGCTGTGCACGCTGCACGCCAACAACGCCAATCAGGCGATG
GACCGCATCATCAACTTCTTCCCCAAAGACCGCCGCAATCAGCTGCTGATGGACCTGTCGCTCAATCTCAAGGGCGTGGT
GGCGCAGCAGCTGATTCCGACGCCGGATGGCCGCAGCCGTCGCGTGGCGATGGAAATCATGTTGGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAAATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACTTGGGCATGCGCACCTTC
GACCAGAGCCTGTTCGAGCTCTACCAGGCTGGCGAAATCAGCTACGAAGACGCATTGCGCTACGCCGACTCGCAGAACGA
AGTGCGCTTGCGCATCAAGCTCTCGCAGGGGGGCGATGCCAAGACCTTGTCGCAGGGCATGGATGGTGTGGAGATTTCCG
AAGTTCGATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(108-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.03

97.606

0.654
  pilU Acinetobacter baylyi ADP1

64.146

94.947

0.609
  pilU Vibrio cholerae strain A1552

53.652

94.681

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.737

94.947

0.396
  pilT Acinetobacter nosocomialis M2

40.116

91.489

0.367
  pilT Acinetobacter baumannii D1279779

40.116

91.489

0.367
  pilT Acinetobacter baumannii strain A118

40.116

91.489

0.367
  pilT Pseudomonas aeruginosa PAK

40.896

89.096

0.364

Multiple sequence alignment