Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACAY30_RS02305 Genome accession   NZ_CP166871
Coordinates   504074..505774 (-) Length   566 a.a.
NCBI ID   WP_290252428.1    Uniprot ID   -
Organism   Thalassotalea ponticola strain KCTC 42155     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 499074..510774
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAY30_RS02280 yacG 499697..499933 (-) 237 WP_290252423.1 DNA gyrase inhibitor YacG -
  ACAY30_RS02285 zapD 500033..500779 (-) 747 WP_290252424.1 cell division protein ZapD -
  ACAY30_RS02290 coaE 500982..501584 (-) 603 WP_290252425.1 dephospho-CoA kinase -
  ACAY30_RS02295 pilD 501701..502606 (-) 906 WP_290252426.1 A24 family peptidase Machinery gene
  ACAY30_RS02300 pilC 502701..503945 (-) 1245 WP_290252427.1 type II secretion system F family protein Machinery gene
  ACAY30_RS02305 pilB 504074..505774 (-) 1701 WP_290252428.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACAY30_RS02310 - 505869..506300 (-) 432 WP_290252429.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACAY30_RS02315 nadC 506956..507849 (-) 894 WP_290252430.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACAY30_RS02320 - 508035..508550 (-) 516 WP_290252431.1 TIGR02281 family clan AA aspartic protease -
  ACAY30_RS02325 ampD 508593..509174 (+) 582 WP_371190241.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACAY30_RS02330 ampE 509400..510245 (+) 846 WP_290252432.1 regulatory signaling modulator protein AmpE -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62000.91 Da        Isoelectric Point: 4.8175

>NTDB_id=1035802 ACAY30_RS02305 WP_290252428.1 504074..505774(-) (pilB) [Thalassotalea ponticola strain KCTC 42155]
MKGIQRQSSILSAVVKHELISRAKATDIYGECQKTGRSVVDYLINKEKLSALELIGVLSKSFGIPVVNLKKLDLTLLPDG
LRNDKLIQKHRALPLYLRGGILYVAVSDPTNVDALEELQFNTGYSTEMVLADDTTLNATIESILEDEQSALDISDLDADE
LGSIAVEDTEKQDDDPQGGENDAPIVVYINKILLDAIKKGASDLHFEPFEKKFRIRFRVDGILTEVATPPVNLASRMTAR
LKVMSKLDIAERRVPQDGRIKLALTKNKSIDFRVSTLPTMWGEKVVMRILDSSSAELGIDILGYEEDQKALYLDALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVQINNKAGLTFAEALKSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLLNMGVPSYNVASSVSLIIAQRLARRLCNHCKEVDDTSPQALIELGFN
PDEVNDITIYRPKGCDECTKGYKGRVGIYEVMKISEENAKLIMDGASSLDIAEQAQKEGYNNLRQSALLKAKAGVTSLAE
VSRVTA

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=1035802 ACAY30_RS02305 WP_290252428.1 504074..505774(-) (pilB) [Thalassotalea ponticola strain KCTC 42155]
ATGAAAGGGATTCAGCGTCAATCAAGTATTCTTTCAGCCGTAGTAAAACACGAGTTGATATCGAGAGCCAAAGCGACCGA
TATATATGGTGAGTGCCAAAAAACAGGGCGCTCTGTGGTTGATTATTTAATTAATAAAGAAAAGCTATCTGCGCTTGAAT
TAATTGGCGTACTGTCAAAAAGCTTTGGTATACCGGTAGTCAACCTTAAAAAGCTCGATTTAACCCTATTACCCGATGGC
CTTCGCAACGATAAGCTGATTCAAAAACACCGCGCTTTACCGCTGTATTTACGCGGTGGTATTTTATATGTGGCGGTATC
CGACCCAACCAATGTTGATGCTCTTGAAGAGTTGCAATTTAATACCGGCTATAGCACCGAAATGGTGCTCGCTGATGACA
CAACCTTAAACGCTACCATTGAATCGATACTAGAAGACGAACAATCAGCACTTGATATTAGCGACTTAGATGCCGATGAG
CTTGGCAGCATAGCGGTTGAAGATACAGAAAAGCAAGACGACGATCCGCAAGGTGGCGAAAACGACGCCCCTATCGTGGT
GTATATTAATAAAATTTTGCTCGATGCGATAAAAAAAGGTGCCTCTGACTTACACTTTGAACCGTTTGAGAAAAAGTTTC
GCATTCGCTTTCGCGTTGACGGTATTTTAACAGAAGTCGCCACTCCACCGGTAAATCTTGCTAGTCGAATGACCGCCCGT
TTAAAGGTTATGTCAAAGCTAGATATTGCCGAGCGCCGTGTACCGCAAGATGGTCGCATTAAACTGGCACTAACCAAAAA
TAAATCGATTGATTTTCGTGTCTCCACCCTGCCTACCATGTGGGGCGAGAAAGTGGTAATGCGTATTTTAGACTCTTCAA
GCGCCGAGCTTGGCATTGATATTTTAGGTTACGAAGAAGACCAAAAAGCTTTGTATTTAGATGCGCTATCAAAACCGCAA
GGGATGATTTTGGTCACTGGGCCAACAGGTTCTGGTAAAACGGTATCGCTTTATACCGGTTTAAATATTTTAAATACAGA
AGAACGCAATATTTCTACCGCCGAAGACCCGGTAGAAATTAATCTTGAAGGGGTTAATCAGGTACAAATTAACAACAAAG
CCGGCTTAACCTTTGCCGAGGCGTTAAAGTCGTTTTTACGACAAGATCCCGATATTGTTATGGTCGGTGAAATACGTGAT
TTAGAAACTGCAGAAATCGCCATTAAAGCGGCGCAAACCGGTCACTTGGTATTGTCTACCCTACATACCAATTCAGCCGC
CGAGACCTTGACTCGTTTGTTAAATATGGGCGTGCCATCTTATAACGTGGCAAGCTCAGTCAGCTTGATTATTGCCCAAC
GCCTTGCGCGCCGATTGTGTAACCACTGTAAGGAGGTAGACGATACCTCGCCGCAAGCGTTAATTGAACTCGGTTTTAAT
CCTGATGAAGTAAACGATATCACCATCTATAGACCCAAAGGCTGTGACGAATGCACCAAGGGTTATAAAGGCCGTGTGGG
GATTTACGAAGTAATGAAAATTAGTGAAGAAAACGCCAAGCTGATTATGGATGGTGCCAGCTCGTTAGATATTGCCGAGC
AAGCGCAAAAAGAAGGTTATAATAACCTGCGCCAGTCGGCTTTATTAAAAGCCAAAGCCGGGGTGACCAGTTTGGCTGAA
GTCAGTCGAGTGACGGCTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-148)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.699

99.823

0.576
  pilB Acinetobacter baylyi ADP1

57.407

95.406

0.548
  pilB Legionella pneumophila strain ERS1305867

54.225

100

0.544
  pilB Vibrio cholerae strain A1552

53.546

99.647

0.534
  pilB Vibrio parahaemolyticus RIMD 2210633

52.289

100

0.525
  pilB Vibrio campbellii strain DS40M4

52.297

100

0.523
  pilF Neisseria gonorrhoeae MS11

48.658

98.763

0.481
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.909

97.173

0.378
  pilB/pilB1 Synechocystis sp. PCC 6803

40.734

91.519

0.373
  pilF Thermus thermophilus HB27

35.517

100

0.364

Multiple sequence alignment