Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ACAY30_RS02295 Genome accession   NZ_CP166871
Coordinates   501701..502606 (-) Length   301 a.a.
NCBI ID   WP_290252426.1    Uniprot ID   -
Organism   Thalassotalea ponticola strain KCTC 42155     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 496701..507606
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAY30_RS02270 mutT 498353..498748 (+) 396 WP_290252421.1 8-oxo-dGTP diphosphatase MutT -
  ACAY30_RS02275 - 499010..499531 (-) 522 WP_290252422.1 DUF3465 domain-containing protein -
  ACAY30_RS02280 yacG 499697..499933 (-) 237 WP_290252423.1 DNA gyrase inhibitor YacG -
  ACAY30_RS02285 zapD 500033..500779 (-) 747 WP_290252424.1 cell division protein ZapD -
  ACAY30_RS02290 coaE 500982..501584 (-) 603 WP_290252425.1 dephospho-CoA kinase -
  ACAY30_RS02295 pilD 501701..502606 (-) 906 WP_290252426.1 A24 family peptidase Machinery gene
  ACAY30_RS02300 pilC 502701..503945 (-) 1245 WP_290252427.1 type II secretion system F family protein Machinery gene
  ACAY30_RS02305 pilB 504074..505774 (-) 1701 WP_290252428.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACAY30_RS02310 - 505869..506300 (-) 432 WP_290252429.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 301 a.a.        Molecular weight: 33295.92 Da        Isoelectric Point: 8.2131

>NTDB_id=1035800 ACAY30_RS02295 WP_290252426.1 501701..502606(-) (pilD) [Thalassotalea ponticola strain KCTC 42155]
MLSDLISLFNQYPVAFYSSCAVLGLLVGSFLNVVIYRLPKMMQAEWACECRELLADELTEEAKKSAKQSAEQSAAMTLSK
PASSCPKCGAKIKPWQNIPVISWLLLRGKCAKCSNPISMRYPIIELLSGLASLVVAMHFGVTWQALFMIVLTWCLIALTM
IDFDHMILPDQITLPLVWLGLILGLNNVFVSLDQAVIGAIAGYMSLWSIYWLFKLATGKEGMGYGDFKLFAVFGAWFGWQ
LLPLLILMASLVGAVIGVSLILFKRHEGSKPIPFGPYLAIAGWICALWGQQIWARYMGLLG

Nucleotide


Download         Length: 906 bp        

>NTDB_id=1035800 ACAY30_RS02295 WP_290252426.1 501701..502606(-) (pilD) [Thalassotalea ponticola strain KCTC 42155]
GTGCTTTCTGATTTGATCTCGCTATTTAACCAATACCCTGTTGCTTTTTATAGTTCCTGCGCAGTGTTAGGCCTCCTCGT
TGGTAGTTTTTTAAATGTGGTCATTTATCGCTTGCCGAAAATGATGCAAGCTGAATGGGCCTGTGAATGTCGTGAGTTGT
TAGCCGATGAATTAACTGAAGAAGCAAAAAAATCTGCGAAACAATCGGCGGAGCAAAGTGCGGCGATGACGTTGTCAAAG
CCAGCATCAAGTTGCCCCAAGTGCGGTGCTAAAATTAAGCCATGGCAAAACATTCCCGTAATCTCGTGGTTGTTACTACG
CGGTAAATGCGCCAAGTGCAGTAACCCAATATCCATGCGTTATCCGATAATTGAACTGCTCAGCGGCCTTGCCTCACTAG
TTGTTGCGATGCACTTTGGTGTGACTTGGCAAGCGCTGTTTATGATTGTTCTTACCTGGTGTTTAATCGCGCTGACCATG
ATTGATTTTGATCATATGATTTTGCCGGATCAAATTACCTTACCACTGGTCTGGCTTGGCTTAATTCTTGGCTTAAATAA
CGTCTTTGTTAGCTTAGACCAAGCAGTCATTGGTGCCATTGCCGGTTATATGAGTTTGTGGTCAATTTATTGGCTATTTA
AGCTAGCCACAGGTAAAGAGGGTATGGGTTATGGCGACTTTAAGCTGTTTGCCGTATTTGGCGCTTGGTTTGGCTGGCAA
CTGTTGCCTTTACTTATTTTAATGGCCTCATTAGTCGGTGCTGTTATTGGCGTGAGTTTAATTTTATTTAAACGCCATGA
AGGGTCAAAGCCCATTCCGTTTGGTCCGTATTTAGCTATAGCTGGTTGGATTTGCGCCCTATGGGGACAACAAATTTGGG
CGCGGTATATGGGGCTTTTGGGTTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(149-258)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(22-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter baumannii D1279779

53.535

98.671

0.528
  pilD Acinetobacter nosocomialis M2

53.199

98.671

0.525
  pilD Vibrio campbellii strain DS40M4

52.083

95.681

0.498
  pilD Vibrio cholerae strain A1552

51.389

95.681

0.492
  pilD Neisseria gonorrhoeae MS11

49.451

90.698

0.449

Multiple sequence alignment