Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QUE89_RS11930 Genome accession   NZ_AP028070
Coordinates   2597132..2598844 (+) Length   570 a.a.
NCBI ID   WP_286220300.1    Uniprot ID   -
Organism   Marinobacter sp. LA51     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2592132..2603844
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUE89_RS11910 - 2592737..2594098 (+) 1362 WP_286220296.1 MBL fold metallo-hydrolase -
  QUE89_RS11915 - 2594095..2594670 (-) 576 WP_286220297.1 pilin -
  QUE89_RS11920 - 2594594..2596123 (-) 1530 WP_286220298.1 O-antigen ligase family protein -
  QUE89_RS11925 - 2596236..2596739 (-) 504 WP_286220299.1 pilin -
  QUE89_RS11930 pilB 2597132..2598844 (+) 1713 WP_286220300.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QUE89_RS11935 pilC 2598847..2600070 (+) 1224 WP_286220301.1 type II secretion system F family protein Machinery gene
  QUE89_RS11940 pilD 2600138..2601013 (+) 876 WP_286220302.1 A24 family peptidase Machinery gene
  QUE89_RS11945 coaE 2601017..2601616 (+) 600 WP_286220303.1 dephospho-CoA kinase -
  QUE89_RS11950 - 2601609..2602706 (+) 1098 WP_286220304.1 hypothetical protein -
  QUE89_RS11955 tsaA 2602709..2603452 (+) 744 WP_286220305.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62577.41 Da        Isoelectric Point: 4.8506

>NTDB_id=103568 QUE89_RS11930 WP_286220300.1 2597132..2598844(+) (pilB) [Marinobacter sp. LA51]
MATNNSNVTLTGLARRFVDDGLLDEATAKDAFLQASQNRIPLITYLVQNNLANSSGLAFSAAMEFGVSVLDLSSFLPEMM
PEKAVDEKLIRKHNALPLYKRGNRLFIAVSDPTNIQALDEIKFNTGLSTDAVLVDDAKLRAAIDKYLESQDTTMGDLDDA
DLEGVETEGGEQEDDGAVSATEVDDAPIVKYVNKMLLDAIRGGASDVHFEPYEKTYRVRYRTDGILKEVSRPSIKLAPKI
SARVKIMAQLDISERRVPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSQAKLGIDVLGYEEDQKEMYMTALE
QPQGMILVTGPTGSGKTVSLYTGLNILNTHERNISTAEDPAEINLEGINQVNVNTRVGLGFAEALRAFLRQDPDVIMVGE
IRDLETANIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPSFNIATSVSLIIAQRLGRRLCSCKQAADVPNDLLLKEG
FTQEQIDTGFTLYRPKGCDKCNGGYKGRVGIYEVVKITDELANMIMEEASSIKIAQQAQAEGFRNLRQSALLKVIEGVTS
LEEANRVTKD

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=103568 QUE89_RS11930 WP_286220300.1 2597132..2598844(+) (pilB) [Marinobacter sp. LA51]
ATGGCTACTAACAACAGCAATGTAACGCTCACCGGTCTGGCAAGACGTTTCGTGGACGACGGACTGCTGGATGAAGCGAC
CGCAAAAGACGCGTTTCTTCAGGCCTCCCAGAATCGCATTCCGTTGATCACCTATCTGGTGCAAAACAACCTTGCGAACA
GCAGCGGATTGGCGTTTTCTGCGGCGATGGAGTTTGGCGTTTCGGTTTTGGACCTGAGCTCCTTTTTGCCAGAAATGATG
CCCGAGAAGGCTGTCGACGAGAAACTGATCCGGAAGCACAACGCTCTTCCCCTATATAAGAGGGGCAATCGTCTGTTTAT
TGCCGTCTCTGATCCCACTAATATTCAGGCCCTTGACGAAATAAAGTTTAATACCGGCCTGAGTACAGATGCGGTCCTGG
TTGACGACGCCAAACTTCGTGCGGCTATCGACAAGTACCTTGAGTCTCAAGACACCACAATGGGCGATCTCGACGATGCC
GATCTGGAAGGCGTCGAGACTGAGGGAGGCGAACAGGAAGACGATGGCGCAGTCTCTGCAACTGAGGTCGATGACGCCCC
CATCGTTAAATATGTAAACAAGATGCTGCTTGACGCCATTCGTGGCGGCGCCTCCGATGTTCACTTTGAGCCTTACGAGA
AAACCTACCGGGTACGTTATCGCACTGACGGTATCCTTAAGGAAGTATCCCGGCCGTCCATCAAACTGGCCCCCAAGATT
TCGGCGCGCGTGAAAATCATGGCACAGCTGGACATTTCGGAGCGCCGGGTGCCCCAGGACGGCCGAATTAAAATGAAGCT
GTCCAAGACCAAAGCCATCGATTTCCGGGTAAACACACTGCCGACCCTTTGGGGCGAGAAGATCGTTCTGCGGATTCTTG
ATCCTAGCCAGGCAAAACTCGGCATTGATGTTCTCGGTTACGAAGAAGACCAGAAAGAGATGTACATGACTGCCCTTGAG
CAACCACAGGGCATGATTCTCGTGACCGGCCCGACCGGTTCGGGCAAGACCGTCTCTCTCTATACAGGCCTTAACATCCT
AAATACCCATGAGCGGAACATTTCTACCGCTGAAGACCCTGCGGAAATAAACCTTGAAGGTATTAACCAGGTTAACGTGA
ATACGCGTGTCGGGCTTGGTTTTGCCGAAGCCCTGCGAGCATTTCTGCGCCAAGACCCCGATGTCATCATGGTTGGTGAG
ATTCGAGACCTCGAAACCGCGAACATTGCGATCAAGGCCGCCCAAACCGGCCACTTAGTTCTCTCAACCCTTCACACCAA
CAGTGCCGCCGAAACACTGACACGAATGATGAACATGGGTGTACCGTCATTCAATATTGCGACGTCAGTGAGCCTGATCA
TTGCCCAGCGCCTTGGCCGGCGCCTTTGTAGCTGCAAGCAGGCGGCAGACGTTCCCAACGACTTGCTTTTGAAGGAAGGG
TTCACACAAGAACAAATTGATACAGGCTTCACGTTGTACCGTCCAAAAGGCTGTGATAAATGCAATGGTGGATACAAAGG
CCGCGTCGGTATCTACGAAGTGGTCAAGATCACCGACGAGCTGGCGAACATGATTATGGAAGAGGCCAGCTCCATTAAAA
TCGCACAGCAGGCTCAGGCCGAAGGCTTCAGAAACTTGCGTCAGTCGGCACTTTTGAAAGTGATTGAGGGTGTCACGAGC
CTCGAAGAAGCCAACCGGGTCACGAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.818

100

0.57
  pilB Acinetobacter baylyi ADP1

56.119

100

0.563
  pilB Legionella pneumophila strain ERS1305867

53.739

100

0.542
  pilB Vibrio parahaemolyticus RIMD 2210633

50.987

97.719

0.498
  pilB Vibrio campbellii strain DS40M4

50

98.947

0.495
  pilB Vibrio cholerae strain A1552

53.95

91.053

0.491
  pilF Neisseria gonorrhoeae MS11

47.527

99.298

0.472
  pilB/pilB1 Synechocystis sp. PCC 6803

40.27

91.053

0.367
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.734

92.281

0.367

Multiple sequence alignment