Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QUE75_RS13430 Genome accession   NZ_AP028062
Coordinates   2936572..2938284 (+) Length   570 a.a.
NCBI ID   WP_286250896.1    Uniprot ID   -
Organism   Marinobacter shengliensis strain D49     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2931572..2943284
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUE75_RS13395 (MAALD49_26500) - 2932451..2932837 (+) 387 WP_286250883.1 glutaredoxin -
  QUE75_RS13400 (MAALD49_26510) - 2932854..2933135 (+) 282 WP_286250885.1 pyrimidine/purine nucleoside phosphorylase -
  QUE75_RS13405 (MAALD49_26520) - 2933205..2933648 (-) 444 WP_286250887.1 pilin -
  QUE75_RS13410 (MAALD49_26530) - 2933747..2934070 (-) 324 WP_106763123.1 DNA-binding transcriptional regulator -
  QUE75_RS13415 (MAALD49_26540) - 2934057..2934383 (-) 327 WP_286250893.1 type II toxin-antitoxin system RelE/ParE family toxin -
  QUE75_RS13420 (MAALD49_26550) - 2934556..2935131 (+) 576 WP_286250894.1 transposase -
  QUE75_RS13425 (MAALD49_26570) - 2935671..2936234 (-) 564 WP_396631659.1 pilin -
  QUE75_RS13430 (MAALD49_26580) pilB 2936572..2938284 (+) 1713 WP_286250896.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QUE75_RS13435 (MAALD49_26590) pilC 2938287..2939507 (+) 1221 WP_106695042.1 type II secretion system F family protein Machinery gene
  QUE75_RS13440 (MAALD49_26600) pilD 2939581..2940456 (+) 876 WP_286250901.1 A24 family peptidase Machinery gene
  QUE75_RS13445 (MAALD49_26610) coaE 2940460..2941065 (+) 606 WP_286250903.1 dephospho-CoA kinase -
  QUE75_RS13450 (MAALD49_26620) - 2941148..2942146 (+) 999 WP_286250906.1 hypothetical protein -
  QUE75_RS13455 (MAALD49_26630) tsaA 2942148..2942897 (+) 750 WP_286250908.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62655.74 Da        Isoelectric Point: 4.9523

>NTDB_id=103471 QUE75_RS13430 WP_286250896.1 2936572..2938284(+) (pilB) [Marinobacter shengliensis strain D49]
MASNNRNVTLTGLARRFVDDGLLDEDTAKDAFLQASQNRIPLITYLTQNHLADSSKLAFSAAMEFGVSVLDLDSFLPEMM
PEKVVDEKLVRKHNALPLYKRGNRLFIAVSDPTNIQALDEIKFNTGLSTDAILVDDAKLRAAIDKYLESQDTTMGDLDDA
DLEGVETEGGDQDDDGAVSATEVDDAPIVKYVNKMLLDAIRGGASDIHFEPYEKIYRVRYRTDGILKEISRPSIKLAPKI
SARVKIMAQLDISERRIPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSQAKLGIDVLGYEEDQKQLYMDALA
QPQGMILVTGPTGSGKTVSLYTGLNILNTPGINISTAEDPAEINLEGINQVNVNTRVGLGFAEALRAFLRQDPDVIMVGE
IRDLETANIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIATSVSLIIAQRLGRRLCSCKQPADVPKDVLLKEG
FTQEQIDTGFTLYRPKGCDKCSSGYKGRVGIYEVVKITEELASMIMEEASSIKIAKQAQAEGFRNLRQSALLKVMEGVTS
LEEANRVTKD

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=103471 QUE75_RS13430 WP_286250896.1 2936572..2938284(+) (pilB) [Marinobacter shengliensis strain D49]
ATGGCGAGCAACAACCGAAACGTGACACTGACAGGCTTGGCCCGTCGATTTGTAGATGACGGGCTTCTGGACGAAGATAC
CGCCAAGGATGCATTCCTTCAGGCTTCCCAGAACCGCATTCCGCTGATCACCTACCTCACCCAGAACCACCTTGCGGACA
GTTCCAAATTGGCGTTTTCTGCTGCCATGGAGTTCGGCGTGTCAGTGCTGGACCTGGATTCTTTCCTGCCGGAAATGATG
CCGGAAAAGGTGGTTGACGAGAAGCTGGTGCGGAAGCATAACGCCCTGCCCCTGTATAAACGAGGCAACAGGCTGTTCAT
CGCCGTATCAGACCCCACAAATATTCAGGCACTGGATGAAATCAAGTTCAACACCGGCTTAAGCACCGATGCGATTCTGG
TGGACGACGCCAAACTTCGCGCTGCCATCGACAAGTACCTCGAATCCCAGGACACCACCATGGGTGACCTGGACGATGCC
GACCTGGAGGGCGTCGAAACTGAGGGCGGCGACCAGGACGACGACGGTGCAGTAAGCGCTACAGAAGTAGATGACGCCCC
GATTGTTAAGTACGTGAACAAGATGCTGCTTGATGCCATTCGTGGTGGTGCTTCCGATATTCACTTCGAGCCTTATGAGA
AAATCTATCGAGTTCGGTACCGGACAGACGGCATCCTCAAAGAGATTTCCCGCCCCTCCATCAAGCTCGCCCCGAAAATC
TCCGCTCGTGTGAAAATCATGGCGCAGCTGGATATCTCCGAACGCCGTATCCCCCAGGACGGCCGGATCAAGATGAAGCT
GTCCAAGACCAAGGCCATCGACTTCCGGGTGAACACGCTGCCCACCCTGTGGGGCGAAAAAATTGTTCTGCGGATTCTGG
ACCCCAGCCAGGCCAAGCTGGGCATCGACGTGCTAGGTTACGAGGAAGACCAGAAGCAACTGTATATGGATGCCCTGGCT
CAGCCCCAGGGTATGATTCTGGTAACCGGGCCAACCGGTTCCGGTAAAACCGTTTCCCTGTACACCGGTCTGAACATTCT
GAACACTCCGGGCATCAACATTTCCACGGCGGAAGACCCAGCGGAAATTAACCTGGAAGGCATTAACCAGGTTAACGTAA
ACACCAGAGTGGGCCTCGGCTTCGCTGAAGCCCTCCGGGCCTTCCTGCGGCAAGACCCGGATGTGATCATGGTGGGTGAG
ATCCGGGATCTGGAGACCGCCAACATTGCGATCAAGGCGGCACAAACCGGACATCTGGTGTTATCAACCCTGCACACCAA
CAGCGCTGCCGAAACTCTGACTCGAATGATGAACATGGGCGTGCCTGCCTTCAACATTGCCACCTCCGTTAGCCTGATCA
TCGCACAGCGCCTGGGCCGGCGCCTGTGCAGCTGCAAACAGCCAGCAGATGTGCCCAAAGACGTGCTTTTGAAGGAAGGG
TTCACACAAGAACAAATTGATACAGGCTTCACGTTGTACCGCCCCAAGGGCTGTGATAAATGCTCTAGTGGATACAAAGG
CCGCGTCGGTATTTACGAAGTGGTGAAGATTACCGAAGAACTGGCGAGCATGATTATGGAAGAGGCCAGTTCCATAAAAA
TTGCAAAACAAGCGCAGGCAGAAGGCTTCCGCAACCTGAGGCAATCCGCCCTTCTGAAAGTAATGGAAGGGGTGACCAGC
CTTGAGGAAGCCAACCGCGTGACGAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-152)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.993

100

0.572
  pilB Acinetobacter baylyi ADP1

57.194

98.772

0.565
  pilB Legionella pneumophila strain ERS1305867

54.087

100

0.546
  pilB Vibrio parahaemolyticus RIMD 2210633

51.706

97.719

0.505
  pilB Vibrio campbellii strain DS40M4

50.709

98.947

0.502
  pilB Vibrio cholerae strain A1552

53.95

91.053

0.491
  pilF Neisseria gonorrhoeae MS11

48.233

99.298

0.479
  pilB/pilB1 Synechocystis sp. PCC 6803

41.233

91.053

0.375
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.354

92.281

0.363
  pilF Thermus thermophilus HB27

37.184

97.193

0.361

Multiple sequence alignment