Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AB8810_RS20010 Genome accession   NZ_CP166096
Coordinates   4633063..4634211 (+) Length   382 a.a.
NCBI ID   WP_179568633.1    Uniprot ID   -
Organism   Xanthomonas sp. NCPPB 3005     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4628063..4639211
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB8810_RS19985 (AB8810_19435) - 4628274..4629461 (-) 1188 WP_191825416.1 cystathionine gamma-synthase -
  AB8810_RS19990 (AB8810_19440) - 4629544..4630914 (-) 1371 WP_369914654.1 pyridoxal-phosphate dependent enzyme -
  AB8810_RS19995 (AB8810_19445) - 4631116..4631340 (-) 225 WP_009575526.1 YdcH family protein -
  AB8810_RS20000 (AB8810_19450) - 4631678..4632574 (-) 897 WP_179568629.1 OmpA family protein -
  AB8810_RS20005 (AB8810_19455) - 4632571..4632963 (-) 393 WP_179568631.1 DUF4398 domain-containing protein -
  AB8810_RS20010 (AB8810_19460) pilU 4633063..4634211 (+) 1149 WP_179568633.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB8810_RS20015 (AB8810_19465) maiA 4634313..4634972 (-) 660 WP_179568635.1 maleylacetoacetate isomerase -
  AB8810_RS20020 (AB8810_19470) - 4635061..4636050 (-) 990 WP_206230133.1 fumarylacetoacetate hydrolase family protein -
  AB8810_RS20025 (AB8810_19475) - 4636426..4637784 (+) 1359 WP_179568644.1 DEAD/DEAH box helicase -
  AB8810_RS20030 (AB8810_19480) queD 4637916..4638275 (+) 360 WP_179568646.1 6-carboxytetrahydropterin synthase QueD -
  AB8810_RS20035 (AB8810_19485) - 4638558..4638929 (+) 372 WP_191825413.1 phasin family protein -

Sequence

Protein


Download         Length: 382 a.a.        Molecular weight: 42330.64 Da        Isoelectric Point: 6.4456

>NTDB_id=1033364 AB8810_RS20010 WP_179568633.1 4633063..4634211(+) (pilU) [Xanthomonas sp. NCPPB 3005]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGATGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALQDAGR
FRVNVFKQRGEVGMVIRAIRSKIPSIEELHLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSQRLVKDKNERRRPATEVLLNTPMIRDLLRRGQVHEIKAAMEESLEEGMETFDQCL
FRMVKQGQIEQEEALRAADSRDGLALKFRLSEGSSGEHDPYADYDAGNSGGVSSPRITHGFG

Nucleotide


Download         Length: 1149 bp        

>NTDB_id=1033364 AB8810_RS20010 WP_179568633.1 4633063..4634211(+) (pilU) [Xanthomonas sp. NCPPB 3005]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAACGCCTCGGACATGTTCCTGACCACGGGAGCACCGGTCTA
TATCAAGATCGAAGGCAAGCTGTACCCGCTCGGCGCCACCGGCCTGCCGCCGGGCATGGTCAAGAAGATCGCCTACTCGC
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAGCTGAACATGGCCATCGCGCTGCAGGACGCCGGCCGC
TTCCGGGTCAACGTGTTCAAGCAGCGCGGCGAGGTCGGCATGGTGATCCGCGCGATCCGCAGCAAGATCCCCAGCATCGA
GGAACTGCACCTGCCGCAGGTGCTGAAGGACGTGATCATGACCCCGCGCGGGCTGGTGCTGGTGGTCGGCTCCACCGGCT
CCGGCAAGTCCACCTCGCTGGCGTCGATGATCGACCACCGCAACAGCACCACCACCGGGCACATCCTCACCATCGAGGAT
CCGATCGAATACCTGCACAAGCACAAGATGTCGATCGTCAACCAGCGCGAGGTCGGCCTGGACACCCACGCCTTCCACAA
CGCACTGAAGAACGCGATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGACGCGACGACGATGGAGGCGG
CGATCGCCTTCGCCGAGACCGGGCATCTGTGCCTGGCCACGCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAGAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGCGCGGTGGTCTCGCAGCG
CCTGGTCAAGGACAAGAACGAGCGCCGCCGCCCGGCCACCGAGGTGCTGCTGAACACGCCGATGATCCGCGACCTGCTGC
GCCGCGGCCAGGTCCACGAGATCAAGGCGGCGATGGAGGAATCGCTGGAGGAAGGCATGGAAACCTTCGACCAGTGCCTG
TTCCGGATGGTCAAGCAGGGCCAGATCGAGCAGGAGGAGGCGCTGCGCGCTGCCGACTCGCGCGACGGCCTGGCGCTGAA
ATTCCGCCTGTCCGAAGGCTCCAGCGGGGAACACGATCCCTACGCCGACTACGACGCCGGCAACAGCGGCGGCGTCAGCT
CGCCGCGGATCACGCACGGCTTCGGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(47-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.253

93.979

0.482
  pilU Vibrio cholerae strain A1552

47.967

96.597

0.463
  pilU Acinetobacter baylyi ADP1

49.153

92.67

0.456
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.531

93.717

0.427
  pilT Pseudomonas aeruginosa PAK

42.012

88.482

0.372
  pilT Acinetobacter baumannii D1279779

40.828

88.482

0.361
  pilT Acinetobacter baumannii strain A118

40.828

88.482

0.361
  pilT Acinetobacter nosocomialis M2

40.828

88.482

0.361

Multiple sequence alignment