Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AACH48_RS19380 Genome accession   NZ_AP027942
Coordinates   4232299..4234014 (+) Length   571 a.a.
NCBI ID   WP_201900045.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain VA84     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4227299..4239014
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH48_RS19355 (VAWG005_37390) pdhR 4228478..4229242 (-) 765 WP_005305630.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  AACH48_RS19360 (VAWG005_37400) ampD 4229518..4230090 (-) 573 WP_019840656.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AACH48_RS19365 (VAWG005_37410) - 4230214..4230684 (+) 471 WP_017779055.1 retropepsin-like aspartic protease -
  AACH48_RS19370 (VAWG005_37420) nadC 4230689..4231546 (+) 858 WP_201900013.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AACH48_RS19375 (VAWG005_37430) - 4231868..4232308 (+) 441 WP_010635961.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  AACH48_RS19380 (VAWG005_37440) pilB 4232299..4234014 (+) 1716 WP_201900045.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  AACH48_RS19385 (VAWG005_37450) pilC 4234130..4235371 (+) 1242 WP_010635960.1 type II secretion system F family protein Machinery gene
  AACH48_RS19390 (VAWG005_37460) pilD 4235394..4236266 (+) 873 WP_019839151.1 A24 family peptidase Machinery gene
  AACH48_RS19395 (VAWG005_37470) coaE 4236286..4236900 (+) 615 WP_019839152.1 dephospho-CoA kinase -
  AACH48_RS19400 (VAWG005_37480) zapD 4236938..4237660 (+) 723 WP_010635957.1 cell division protein ZapD -
  AACH48_RS19405 (VAWG005_37490) yacG 4237670..4237864 (+) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  AACH48_RS19410 (VAWG005_37500) mutT 4237925..4238338 (-) 414 WP_005305665.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62841.38 Da        Isoelectric Point: 5.4363

>NTDB_id=103063 AACH48_RS19380 WP_201900045.1 4232299..4234014(+) (pilB) [Aeromonas dhakensis strain VA84]
MPIMTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIP
QKYLNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLMESDQDALGMEEIDE
SEISELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEVATPPVNLANRF
SARLKVMARLDIAERRLPQDGRIKLKMSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALS
KPQGMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGE
IRDLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLEL
GFTQQQLAAGLRLFKPVGCKECSGGYKGRVGIYEILLMSENIAKLIMQGANSMQIAAIAQKEGMRTLRTSGLEKARLGVT
SLAEINRVTTN

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=103063 AACH48_RS19380 WP_201900045.1 4232299..4234014(+) (pilB) [Aeromonas dhakensis strain VA84]
CTGCCCATAATGACATCTAGTCCCAATAGCGGCCTGGCATTAAGCTTGGCCGCCTCTTCCCTGCTCAGTGAGTCCGACTC
ACAGCGCTATCTGAGCCAGGCCAAGGCGCAGCGCAAGCCCTTTGTGACCTTTCTGATCGAGAACGAAATCCTCGACAGCA
AGGCGCTGGCCGACTTCTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCG
CAGAAGTACCTCAATCAGAAGCTGATTGAGAAACACCATGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGC
CATGTCCGACCCGACCAATGTGTCGGCACTGGAGGATTTTGGCTTCAGCTTCGGGCTGCACACCGAGGCCTTGCTGGTCG
AGGAGAACAAGCTGACCGCCGCCATCGGCAAGCTGATGGAGAGCGATCAGGACGCCCTCGGCATGGAGGAGATCGACGAA
TCCGAGATCTCCGAGCTGGAGGTGTCTGACGAGGGGTCACGACTCGACGAGAGCGTCAACACGGCGGACGATGACGCTCC
CATCGTCAAATACATCAACAAGATCATGATGGATGCCATCAAGCGCGGTGCATCCGACCTCCACTTCGAGCCCTATGAGA
CCAAGTACCGTATTCGCTTTCGGATAGACGGGATCCTGCATGAGGTAGCAACGCCGCCGGTCAATCTGGCCAACCGCTTC
TCGGCCCGACTCAAGGTGATGGCGCGCCTGGACATCGCCGAACGGCGGCTGCCCCAGGATGGCCGCATCAAATTGAAAAT
GTCGCGCAACAAGTCGATGGACATGCGGGTCAACACCCTGCCTACCATGTGGGGCGAGAAGATCGTGATCCGTCTGCTCG
ACTCTTCCGCTGCGCGCCTCAATATCGAACAGCTCGGCTTTGACGATCGGCAGAAGGCACAATATCTGCGCGCGCTCTCG
AAGCCTCAGGGCATGATCCTGGTGACAGGCCCGACCGGTTCGGGCAAGACGGTGTCGCTCTACACCGGCCTGAACATCCT
CAACACCACAGAGGTGAATATTTCGACCGCCGAAGACCCAGTGGAGATCAACCTGCCTGGGGTCAATCAGGTACAGGTCA
ACCCCAAGGCCGGGCTGACCTTCGCCAGTGCCCTGCGCTCCTTCCTGCGCCAGGATCCCGATGTGGTGATGGTCGGGGAA
ATACGGGATCTTGAAACCGCCGAGATCGCCATCAAGGCGGCGCAAACCGGCCACCTGGTGCTCTCTACATTGCACACCAA
CTCGGCGGCCGAGACGCTGACCCGGATGATGAACATGGGAGTCCCCGCCTTCAACATCGCCTCCTCGGTTACCCTGATCA
TGGCTCAGCGACTGGCACGCAAGCTCTGTGACCACTGCAAGGCTCCCGAAGTGGTGCCGGAGGCTGAACTGCTGGAGTTG
GGGTTCACCCAACAGCAGCTGGCCGCCGGCTTGCGGCTGTTCAAGCCGGTCGGCTGCAAGGAGTGTTCTGGCGGCTACAA
GGGGCGGGTCGGCATCTACGAGATCCTGTTGATGTCAGAAAATATTGCAAAATTGATCATGCAGGGAGCCAACTCCATGC
AGATTGCGGCCATCGCCCAGAAGGAGGGCATGCGCACCTTGCGGACCTCCGGTCTTGAGAAGGCCCGGCTGGGGGTGACC
AGCCTGGCCGAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-149)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.957

100

0.553
  pilB Acinetobacter baylyi ADP1

55.148

100

0.553
  pilB Legionella pneumophila strain ERS1305867

54.594

99.124

0.541
  pilB Vibrio cholerae strain A1552

54.159

98.949

0.536
  pilB Vibrio campbellii strain DS40M4

51.767

99.124

0.513
  pilB Vibrio parahaemolyticus RIMD 2210633

51.068

98.424

0.503
  pilF Neisseria gonorrhoeae MS11

46.454

98.774

0.459
  pilB/pilB1 Synechocystis sp. PCC 6803

38.698

96.848

0.375
  pilF Thermus thermophilus HB27

40.53

92.469

0.375

Multiple sequence alignment