Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AB6N02_RS01015 Genome accession   NZ_CP163510
Coordinates   200919..202625 (+) Length   568 a.a.
NCBI ID   WP_052814466.1    Uniprot ID   A0AAW9FA05
Organism   Aeromonas caviae strain 103     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 195919..207625
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB6N02_RS00990 (AB6N02_00990) pdhR 197140..197904 (-) 765 WP_052814468.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  AB6N02_RS00995 (AB6N02_00995) ampD 198181..198753 (-) 573 WP_039040507.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AB6N02_RS01000 (AB6N02_01000) - 198882..199352 (+) 471 WP_042015101.1 TIGR02281 family clan AA aspartic protease -
  AB6N02_RS01005 (AB6N02_01005) nadC 199356..200219 (+) 864 WP_052814467.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AB6N02_RS01010 (AB6N02_01010) - 200380..200919 (+) 540 WP_270825191.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  AB6N02_RS01015 (AB6N02_01015) pilB 200919..202625 (+) 1707 WP_052814466.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  AB6N02_RS01020 (AB6N02_01020) pilC 202753..203994 (+) 1242 WP_010675822.1 type II secretion system F family protein Machinery gene
  AB6N02_RS01025 (AB6N02_01025) pilD 204072..204947 (+) 876 WP_052814465.1 A24 family peptidase Machinery gene
  AB6N02_RS01030 (AB6N02_01030) coaE 204966..205580 (+) 615 WP_369325401.1 dephospho-CoA kinase -
  AB6N02_RS01035 (AB6N02_01035) zapD 205618..206340 (+) 723 WP_010675825.1 cell division protein ZapD -
  AB6N02_RS01040 (AB6N02_01040) yacG 206351..206545 (+) 195 WP_039040499.1 DNA gyrase inhibitor YacG -
  AB6N02_RS01045 (AB6N02_01045) mutT 206603..207007 (-) 405 WP_174213873.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62564.93 Da        Isoelectric Point: 5.8214

>NTDB_id=1030210 AB6N02_RS01015 WP_052814466.1 200919..202625(+) (pilB) [Aeromonas caviae strain 103]
MTSSLNSGLATSLAASSLLSEPDSQRYLSQARAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEESKLTAAIGKLMESEQDTLGMDHIDEAEI
SELEVSDENSRLDESVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRVDGILHEVATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRSRSMDMRVNTLPTMWGEKIVIRLLDSSAARLNLDQLGFDDRQKAQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSDNIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=1030210 AB6N02_RS01015 WP_052814466.1 200919..202625(+) (pilB) [Aeromonas caviae strain 103]
ATGACCTCCTCACTCAATAGCGGCCTGGCCACCAGCCTGGCCGCTTCCTCCCTCCTCAGTGAGCCGGACTCACAGCGTTA
TCTGAGCCAGGCCAGGGCGCAGCGCAAGCCCTTTGTCACCTTCCTGATCGAGAACGATATCCTCGACAGCAAGGCGCTGG
CAGATTTCTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTGGCGGCCTTCGATCTCGCCGAGATCCCGCAGAAATAC
CTCAATCAAAAGCTGATTGAAAAGCACCATGTCCTGCCCATCTACACCCAGGGGCATACCCTCTATATCGCCATGTCCGA
TCCGACCAATGTGTCGGCTCTGGAGGATTTTGGTTTCAGTTTTGGCCTGCACACGGAAGCCCTGCTGGTCGAAGAGAGCA
AGCTGACCGCCGCCATCGGCAAGCTGATGGAGAGCGAGCAGGATACCCTCGGCATGGATCACATCGACGAGGCCGAGATC
TCGGAGCTCGAGGTCTCCGATGAGAATTCCCGCCTCGACGAGAGCGTCAACACCGCCGACGACGATGCCCCCATCGTCAA
ATACATCCATAAAATCATGATGGACGCCATCAAGCGCGGGGCATCCGACCTGCATTTCGAACCCTATGAGACCAAGTACC
GGATCCGCTTTCGGGTAGACGGCATACTGCACGAAGTCGCCACCCCGCCGGTCAATCTGGCGAACCGTTTCGCGGCTCGC
TTAAAGGTGATGGCGAGGCTGGATATCGCCGAGCGGCGTCTGCCCCAGGATGGGCGGATCAAGCTGAAGATCTCCCGCAG
CAGATCCATGGACATGCGGGTCAATACCCTGCCCACCATGTGGGGCGAGAAGATCGTCATCCGCTTGCTGGACTCGTCGG
CCGCCCGCCTCAACCTCGATCAACTCGGCTTCGACGACCGCCAAAAGGCGCAATACCTGCATGCGCTCTCCAAACCACAG
GGCATGATACTGGTCACCGGGCCCACAGGGTCGGGCAAGACGGTGTCGCTCTATACCGGTCTCAACATCCTCAATACCAG
CGAGGTCAACATCTCCACCGCCGAGGATCCGGTTGAGATCAACCTGCCCGGCGTCAACCAGGTGCAGATCAACCCCAAGG
CGGGGCTCACCTTCGCCAGTGCCCTGCGCTCCTTCCTGCGCCAGGATCCCGACATCGTCATGGTGGGGGAGATCCGGGAT
CTGGAAACCGCCGAGATTGCCATCAAGGCCGCCCAGACCGGTCACCTGGTGCTGTCGACCCTGCACACCAACTCGGCGGC
AGAAACGCTCACCCGGATGATGAACATGGGGGTGCCGGCCTTCAACATCGCCTCTTCGGTCACCCTCATCATGGCCCAGC
GCCTTGCCCGCAAACTCTGCGATCACTGCAAGGCGCCGGAAGTGGTGCCGGAAGCCGAGTTGCTGGAGCTGGGCTTCACC
CAGCAGCAACTGGCCGCCGGTTTGCGGCTGTTCAAACCCGTGGGATGCAAGGAGTGCTCGGGAGGCTACAGAGGCCGGGT
CGGCATTTATGAGATCATGCTGATGTCCGACAACATCGCCAAACTCATCATGCAGGGAGCCAACTCGCTGCAGATTGCCG
CGATAGCCCAAAAAGAAGGGATGCGCACCCTGCGTACATCCGGCCTCGAGAAAGCGCGTCTTGGCGTCACCAGCCTGGCC
GAAATCAACAGGATCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.026

99.824

0.549
  pilB Acinetobacter baylyi ADP1

55.062

99.12

0.546
  pilB Legionella pneumophila strain ERS1305867

54.69

99.472

0.544
  pilB Vibrio cholerae strain A1552

53.24

100

0.535
  pilB Vibrio campbellii strain DS40M4

50.177

99.648

0.5
  pilB Vibrio parahaemolyticus RIMD 2210633

49.734

99.12

0.493
  pilF Neisseria gonorrhoeae MS11

46.384

99.824

0.463
  pilB/pilB1 Synechocystis sp. PCC 6803

38.156

97.359

0.371
  pilF Thermus thermophilus HB27

39.962

92.958

0.371

Multiple sequence alignment