Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AACH36_RS18650 Genome accession   NZ_AP027939
Coordinates   4094767..4096473 (+) Length   568 a.a.
NCBI ID   WP_042025094.1    Uniprot ID   -
Organism   Aeromonas enteropelogenes strain VA111     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4089767..4101473
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH36_RS18625 (VAWG006_36470) pdhR 4090988..4091752 (-) 765 WP_026456603.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  AACH36_RS18630 (VAWG006_36480) ampD 4092019..4092600 (-) 582 WP_338611356.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AACH36_RS18635 (VAWG006_36490) - 4092624..4093094 (+) 471 WP_338611357.1 retropepsin-like aspartic protease -
  AACH36_RS18640 (VAWG006_36500) nadC 4093098..4093955 (+) 858 WP_201941486.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AACH36_RS18645 (VAWG006_36510) - 4094330..4094767 (+) 438 WP_201941484.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  AACH36_RS18650 (VAWG006_36520) pilB 4094767..4096473 (+) 1707 WP_042025094.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  AACH36_RS18655 (VAWG006_36530) pilC 4096695..4097936 (+) 1242 WP_026456598.1 type II secretion system F family protein Machinery gene
  AACH36_RS18660 (VAWG006_36540) pilD 4098003..4098878 (+) 876 WP_338611358.1 A24 family peptidase Machinery gene
  AACH36_RS18665 (VAWG006_36550) coaE 4098882..4099496 (+) 615 WP_223379351.1 dephospho-CoA kinase -
  AACH36_RS18670 (VAWG006_36560) zapD 4099534..4100256 (+) 723 WP_061476588.1 cell division protein ZapD -
  AACH36_RS18675 (VAWG006_36570) yacG 4100266..4100460 (+) 195 WP_026457308.1 DNA gyrase inhibitor YacG -
  AACH36_RS18680 (VAWG006_36580) mutT 4100523..4100918 (-) 396 WP_042071005.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62535.94 Da        Isoelectric Point: 5.5619

>NTDB_id=103007 AACH36_RS18650 WP_042025094.1 4094767..4096473(+) (pilB) [Aeromonas enteropelogenes strain VA111]
MTSSPHSGLAISLAASSLISEEDSQRYLSQAKAQRKPFVAFLVENQILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTTAIGKLLENETDVLGMEDIDESEI
SDLEVSDEGSRLDESVNTTDDDAPIVKYINKILMDAIKRGASDLHFEPYEQKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
PQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=103007 AACH36_RS18650 WP_042025094.1 4094767..4096473(+) (pilB) [Aeromonas enteropelogenes strain VA111]
ATGACCTCTAGCCCCCATAGCGGCCTGGCGATAAGCCTGGCCGCCTCTTCGTTGATCAGTGAAGAGGATTCCCAGCGCTA
CCTGAGTCAGGCCAAGGCCCAGCGCAAACCCTTCGTGGCATTTCTGGTGGAGAACCAGATCCTCGACAGCAAGGCGTTGG
CTGATTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGATCTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGTTGATTGAAAAACACCATGTGCTGCCCATCTACACCCAGGGCCACACTCTCTATATCGCCATGTCGGA
TCCGACCAATGTGTCGGCGCTGGAAGACTTCGGCTTCAGCTTCGGGCTTCATACCGAGGCGCTGCTGGTGGAGGAGAACA
AGCTCACCACCGCCATCGGCAAGCTGCTGGAAAACGAGACCGACGTGCTCGGAATGGAGGATATCGACGAGTCCGAAATC
TCGGATCTTGAGGTCTCCGACGAGGGCAGTCGCCTCGACGAGAGTGTCAACACCACGGACGATGACGCCCCCATCGTCAA
GTACATCAACAAAATCCTGATGGATGCCATCAAGCGCGGCGCCTCCGACCTGCACTTCGAGCCCTATGAGCAGAAGTACC
GCATCCGCTTTCGCATCGATGGCATCCTGCACGAAATCGCCACGCCGCCGGTCAATCTGGCCAACCGCTTCTCGGCCCGT
CTCAAGGTGATGGCACGGCTCGATATCGCCGAGCGGCGCCTGCCCCAGGATGGCCGCATCAAGCTGAAGCTGTCGCGCAA
CAAGTCGATGGACATGCGAGTCAATACCCTGCCCACCATGTGGGGGGAGAAGATCGTTATCCGCCTGCTGGACTCCTCGG
CGGCGCGCCTCAATATCGAGCAGCTCGGCTTTGACGACAGGCAGAAAGCGCAATACCTGCGCGCCCTCTCCAAACCCCAG
GGAATGATCCTGGTGACCGGCCCCACCGGCTCGGGCAAAACGGTCTCGCTCTACACCGGCCTCAACATTCTCAACACCAC
AGAGGTAAATATCTCCACCGCCGAGGATCCGGTGGAGATCAACCTGCCCGGCGTCAATCAGGTGCAGGTCAATCCCAAGG
CGGGACTCACCTTCGCCAGCGCCCTGCGCTCCTTCCTGCGTCAGGATCCTGATGTTGTGATGGTCGGTGAAATACGGGAC
TTGGAAACCGCCGAGATCGCCATCAAAGCGGCGCAGACCGGCCACTTGGTGCTCTCCACTCTGCACACTAACTCCGCTGC
CGAAACCCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAACATCGCCTCCTCAGTGACCCTGATCATGGCCCAGC
GGCTGGCCCGCAAGCTATGTGATCACTGCAAGGCGCCCGAAGTGGTGCCGGAGGCCGAATTGCTGGAGCTGGGCTTTACC
CCGCAGCAACTGGCAGCGGGGCTGCGGCTGTTCAAACCGGTAGGTTGCAAGGAGTGCTCTGGCGGTTACAAGGGGCGGGT
CGGCATCTACGAAATTATGCTGATGTCGGAAAATATCGCAAAATTGATCATGCAGGGAGCCAACTCGCTGCAGATCGCCG
CCATCGCTCAGAAAGAGGGGATGCGGACCCTGCGCACCTCCGGGTTGGAAAAAGCCCGCCTGGGTGTCACCAGTCTGGCG
GAAGTGAACCGGGTCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.415

100

0.565
  pilB Acinetobacter baylyi ADP1

55.81

100

0.558
  pilB Legionella pneumophila strain ERS1305867

56.827

95.423

0.542
  pilB Vibrio cholerae strain A1552

53.996

99.12

0.535
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

99.296

0.512
  pilB Vibrio campbellii strain DS40M4

50.348

100

0.509
  pilF Neisseria gonorrhoeae MS11

46.986

99.296

0.467
  pilB/pilB1 Synechocystis sp. PCC 6803

38.062

100

0.387
  pilF Thermus thermophilus HB27

40.341

92.958

0.375

Multiple sequence alignment