Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AACH59_RS07985 Genome accession   NZ_AP027936
Coordinates   1733818..1734957 (-) Length   379 a.a.
NCBI ID   WP_017766703.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain VA54     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1728818..1739957
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH59_RS07965 (VAWG003_15330) feoB 1729039..1731312 (+) 2274 WP_201900388.1 Fe(2+) transporter permease subunit FeoB -
  AACH59_RS07970 (VAWG003_15340) - 1731309..1731551 (+) 243 WP_005302637.1 FeoC-like transcriptional regulator -
  AACH59_RS07975 (VAWG003_15350) - 1731673..1732152 (-) 480 WP_010633537.1 WbuC family cupin fold metalloprotein -
  AACH59_RS07980 (VAWG003_15360) glgC 1732288..1733559 (-) 1272 WP_026459078.1 glucose-1-phosphate adenylyltransferase -
  AACH59_RS07985 (VAWG003_15370) pilU 1733818..1734957 (-) 1140 WP_017766703.1 type IVa pilus ATPase TapW Machinery gene
  AACH59_RS07990 (VAWG003_15380) - 1735017..1735373 (-) 357 WP_005302630.1 DMT family protein -
  AACH59_RS07995 (VAWG003_15390) - 1735487..1736218 (+) 732 WP_024942579.1 transporter substrate-binding domain-containing protein -
  AACH59_RS08000 (VAWG003_15400) btuC 1736318..1737325 (+) 1008 WP_323997658.1 vitamin B12 ABC transporter permease BtuC -
  AACH59_RS08005 (VAWG003_15410) - 1737312..1738097 (+) 786 WP_162519030.1 ATP-binding cassette domain-containing protein -
  AACH59_RS08010 (VAWG003_15420) - 1738177..1739238 (-) 1062 WP_005302620.1 3-deoxy-7-phosphoheptulonate synthase -
  AACH59_RS08015 (VAWG003_15430) - 1739395..1739775 (+) 381 WP_005302618.1 diacylglycerol kinase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42171.19 Da        Isoelectric Point: 6.2561

>NTDB_id=102936 AACH59_RS07985 WP_017766703.1 1733818..1734957(-) (pilU) [Aeromonas dhakensis strain VA54]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLSETPLEPGEVASIADAIMDGEQKQQFEKELEMNLAISLPQIGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPTVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMVKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIDEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKDDLF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=102936 AACH59_RS07985 WP_017766703.1 1733818..1734957(-) (pilU) [Aeromonas dhakensis strain VA54]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGACGGCTCAGACCTCTATCTGTCGACCGGCGCGCCCCCCTG
TGCCAAGTTCAACGGCGGCCTGCGCCCCTTGAGCGAGACGCCACTCGAGCCCGGCGAGGTGGCCAGCATAGCCGACGCCA
TCATGGACGGCGAGCAGAAGCAGCAGTTTGAAAAGGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATAGGGCGC
TTTCGGATCAACATCTTCAAGCAGCGCAACGAAGTCTCCCTGGTGGCGCGCAACATCAAGACCGAGATCCCTCGCTTCGA
GGACTTGAAACTGCCCACCGTGCTGCTCGACACCATCATGGAGAAACGGGGGCTGGTGCTGTTCGTCGGCGGCACCGGTT
CGGGCAAATCCACCTCGCTGGCGGCCCTTATCGACCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTGCACCGCCACCGCAAGAGCATCATCAACCAGCGGGAGGTGGGGGTGGATACCCGCAGCTTCCACGC
CGCCCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCCTTATCGGCGAAATTCGCGATCGCGAGACCATGGAGCACG
CGCTGGCCTTCTCGGAAACCGGCCACCTGGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAAGAGCGCCGGCCCCAGCTGCTCAACGATCTTGGCAACAACCTCAAGGCGTTTGTCTCCCAGCG
GCTGGTGAAAACCACTGATGGCGGCCGCCGGGCCGCGGTGGAGATCATGCTGGGTACCCACACCATCCGCGACATGGTCA
AGCGCGGCGAGTTTGGCGGCCTCAAGGAGGTGATGGAGAAGTCCAAGGCCCTCGGCATGGTCACCTTCGACAGTGCCCTG
TTCGATCTGGTGGTGGAAGGGGTGATCGACGAGGAGGAAGCGGTGAAGAACGCCGATTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTGTGGAAGGAGAAGGGCCAGATCGCCAGCAGCAGCGATGCCACCGGCTGGAGCCTGGAGCCCACCAAGGACG
AAAAGGACGATCTGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

53.867

98.945

0.533
  pilU Acinetobacter baylyi ADP1

52.958

93.668

0.496
  pilU Vibrio cholerae strain A1552

50

92.348

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.793

93.14

0.38
  pilT Pseudomonas aeruginosa PAK

40.407

90.765

0.367
  pilT Legionella pneumophila strain ERS1305867

41.317

88.127

0.364
  pilT Legionella pneumophila strain Lp02

41.317

88.127

0.364
  pilT Pseudomonas stutzeri DSM 10701

39.826

90.765

0.361

Multiple sequence alignment