Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AACH72_RS11970 Genome accession   NZ_AP027931
Coordinates   2642203..2643918 (+) Length   571 a.a.
NCBI ID   WP_185450531.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain VA6     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2637203..2648918
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH72_RS11945 (VAWG001_23280) pdhR 2638382..2639146 (-) 765 WP_005305630.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  AACH72_RS11950 (VAWG001_23290) ampD 2639422..2639994 (-) 573 WP_208200766.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AACH72_RS11955 (VAWG001_23300) - 2640118..2640588 (+) 471 WP_043171614.1 retropepsin-like aspartic protease -
  AACH72_RS11960 (VAWG001_23310) nadC 2640593..2641450 (+) 858 WP_043171615.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AACH72_RS11965 (VAWG001_23320) - 2641772..2642212 (+) 441 WP_010635961.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  AACH72_RS11970 (VAWG001_23330) pilB 2642203..2643918 (+) 1716 WP_185450531.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  AACH72_RS11975 (VAWG001_23340) pilC 2644034..2645275 (+) 1242 WP_010635960.1 type II secretion system F family protein Machinery gene
  AACH72_RS11980 (VAWG001_23350) pilD 2645298..2646170 (+) 873 WP_213344272.1 A24 family peptidase Machinery gene
  AACH72_RS11985 (VAWG001_23360) coaE 2646190..2646804 (+) 615 WP_017766418.1 dephospho-CoA kinase -
  AACH72_RS11990 (VAWG001_23370) zapD 2646842..2647564 (+) 723 WP_026141277.1 cell division protein ZapD -
  AACH72_RS11995 (VAWG001_23380) yacG 2647574..2647768 (+) 195 WP_213344273.1 DNA gyrase inhibitor YacG -
  AACH72_RS12000 (VAWG001_23390) mutT 2647829..2648242 (-) 414 WP_005305665.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62871.40 Da        Isoelectric Point: 5.4363

>NTDB_id=102887 AACH72_RS11970 WP_185450531.1 2642203..2643918(+) (pilB) [Aeromonas dhakensis strain VA6]
MPIMTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIP
QKYLNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAISKLMESDQDALGMEEIDE
SEISELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEVATPPVNLANRF
SARLKVMARLDIAERRLPQDGRIKLKMSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALS
KPQGMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGE
IRDLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLEL
GFTQQQLAAGLRLFKPVGCKECSGGYKGRVGIYEILLMSENIAKLIMQGANSMQIAAIAQKEGMRTLRTSGLEKARLGVT
SLAEINRVTTN

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=102887 AACH72_RS11970 WP_185450531.1 2642203..2643918(+) (pilB) [Aeromonas dhakensis strain VA6]
CTGCCCATAATGACATCTAGTCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCCCTGCTCAGTGAGTCCGACTC
ACAGCGCTATCTGAGCCAGGCCAAGGCGCAGCGCAAGCCCTTTGTGACCTTTCTGATCGAGAACGAAATCCTCGACAGCA
AGGCGCTGGCCGACTTCTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCG
CAGAAGTACCTCAATCAGAAGCTGATTGAGAAACACCATGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGC
CATGTCCGACCCGACCAATGTGTCGGCACTGGAGGATTTTGGCTTCAGCTTCGGGCTGCACACCGAGGCCTTGCTGGTCG
AGGAGAACAAGCTGACCGCCGCCATCAGCAAGCTGATGGAGAGCGATCAGGACGCCCTTGGCATGGAGGAGATCGACGAG
TCCGAGATTTCCGAGCTGGAAGTGTCTGACGAGGGGTCACGACTCGACGAGAGCGTCAACACGGCGGACGATGACGCCCC
CATCGTCAAATACATCAACAAGATCATGATGGATGCCATCAAGCGCGGTGCATCCGACCTCCACTTCGAGCCCTATGAGA
CCAAGTACCGTATTCGCTTTCGGATAGACGGGATCCTGCATGAGGTAGCAACGCCGCCGGTCAATCTGGCCAACCGCTTC
TCGGCCCGACTCAAGGTGATGGCGCGCCTGGACATCGCCGAACGGCGGCTGCCCCAGGATGGCCGCATCAAATTGAAAAT
GTCGCGCAACAAGTCGATGGACATGCGGGTCAACACCCTGCCTACCATGTGGGGCGAGAAGATCGTGATCCGTCTGCTCG
ACTCTTCCGCTGCGCGCCTCAATATCGAGCAGCTCGGCTTCGACGATCGGCAGAAGGCGCAATATCTGCGTGCGCTCTCG
AAGCCTCAGGGCATGATCCTGGTGACCGGTCCGACCGGCTCGGGCAAGACGGTGTCGCTCTACACCGGCCTGAACATCCT
CAACACCACAGAGGTGAATATTTCGACCGCCGAAGACCCGGTGGAGATCAACCTGCCAGGAGTCAATCAGGTGCAGGTCA
ACCCCAAGGCAGGGCTCACCTTCGCCAGTGCCCTGCGCTCCTTCCTGCGCCAGGATCCCGACGTGGTGATGGTCGGGGAA
ATACGGGATCTAGAAACCGCCGAGATCGCCATCAAGGCGGCACAAACCGGTCACCTGGTGCTTTCCACATTGCACACCAA
CTCGGCAGCCGAGACGCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAACATCGCCTCCTCGGTTACCCTGATCA
TGGCTCAGCGACTGGCACGCAAGCTCTGTGACCACTGCAAGGCTCCCGAAGTGGTGCCGGAGGCTGAACTGCTGGAGTTG
GGTTTTACCCAACAGCAGCTGGCAGCAGGCTTGCGGCTGTTCAAGCCGGTTGGCTGCAAGGAGTGTTCTGGCGGCTACAA
GGGACGGGTCGGCATCTACGAGATCCTGTTGATGTCAGAAAATATTGCAAAATTGATCATGCAGGGAGCCAACTCTATGC
AGATTGCGGCCATTGCCCAGAAGGAGGGCATGCGGACCTTGCGTACCTCCGGTCTTGAGAAGGCCCGACTGGGGGTGACC
AGCCTGGCCGAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.323

100

0.555
  pilB Acinetobacter baumannii D1279779

54.783

100

0.552
  pilB Legionella pneumophila strain ERS1305867

54.594

99.124

0.541
  pilB Vibrio cholerae strain A1552

54.159

98.949

0.536
  pilB Vibrio campbellii strain DS40M4

51.767

99.124

0.513
  pilB Vibrio parahaemolyticus RIMD 2210633

51.068

98.424

0.503
  pilF Neisseria gonorrhoeae MS11

46.454

98.774

0.459
  pilB/pilB1 Synechocystis sp. PCC 6803

38.698

96.848

0.375
  pilF Thermus thermophilus HB27

40.53

92.469

0.375

Multiple sequence alignment