Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AACH72_RS00255 Genome accession   NZ_AP027931
Coordinates   47687..48826 (-) Length   379 a.a.
NCBI ID   WP_017766703.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain VA6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 42687..53826
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH72_RS00235 (VAWG001_00470) feoB 42882..45155 (+) 2274 WP_139751945.1 Fe(2+) transporter permease subunit FeoB -
  AACH72_RS00240 (VAWG001_00480) - 45152..45394 (+) 243 WP_005302637.1 FeoC-like transcriptional regulator -
  AACH72_RS00245 (VAWG001_00490) - 45518..45997 (-) 480 WP_162519029.1 WbuC family cupin fold metalloprotein -
  AACH72_RS00250 (VAWG001_00500) glgC 46133..47404 (-) 1272 WP_005302634.1 glucose-1-phosphate adenylyltransferase -
  AACH72_RS00255 (VAWG001_00510) pilU 47687..48826 (-) 1140 WP_017766703.1 type IVa pilus ATPase TapW Machinery gene
  AACH72_RS00260 (VAWG001_00520) - 48886..49242 (-) 357 WP_005302630.1 DMT family protein -
  AACH72_RS00265 (VAWG001_00530) - 49356..50087 (+) 732 WP_208201292.1 transporter substrate-binding domain-containing protein -
  AACH72_RS00270 (VAWG001_00540) btuC 50187..51194 (+) 1008 WP_042052043.1 vitamin B12 ABC transporter permease BtuC -
  AACH72_RS00275 (VAWG001_00550) - 51181..51966 (+) 786 WP_192897132.1 ATP-binding cassette domain-containing protein -
  AACH72_RS00280 (VAWG001_00560) - 52046..53107 (-) 1062 WP_005302620.1 3-deoxy-7-phosphoheptulonate synthase -
  AACH72_RS00285 (VAWG001_00570) - 53264..53644 (+) 381 WP_005302618.1 diacylglycerol kinase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42171.19 Da        Isoelectric Point: 6.2561

>NTDB_id=102874 AACH72_RS00255 WP_017766703.1 47687..48826(-) (pilU) [Aeromonas dhakensis strain VA6]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLSETPLEPGEVASIADAIMDGEQKQQFEKELEMNLAISLPQIGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPTVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMVKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIDEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKDDLF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=102874 AACH72_RS00255 WP_017766703.1 47687..48826(-) (pilU) [Aeromonas dhakensis strain VA6]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGACGGCTCAGACCTCTATCTGTCGACCGGCGCGCCCCCCTG
TGCCAAGTTCAACGGCGGCCTGCGCCCCTTGAGCGAGACGCCGCTCGAGCCCGGCGAGGTGGCCAGCATAGCCGACGCCA
TCATGGACGGCGAGCAGAAGCAGCAGTTTGAGAAGGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATAGGGCGC
TTTCGGATCAACATCTTCAAGCAGCGCAACGAAGTCTCCCTGGTGGCGCGCAACATCAAGACCGAGATCCCTCGCTTCGA
GGACTTGAAACTGCCCACCGTGCTGCTCGACACCATCATGGAGAAACGGGGGCTGGTGCTGTTCGTCGGCGGCACCGGTT
CGGGCAAATCCACCTCGCTCGCAGCCCTTATCGACCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTGCACCGCCACCGCAAGAGCATCATCAACCAGCGAGAGGTGGGGGTGGATACCCGCAGCTTCCACGC
CGCCCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCCTTATCGGCGAAATTCGCGATCGCGAAACCATGGAGCATG
CGCTGGCCTTCTCGGAGACCGGCCACCTGGCCATCTCCACCCTGCACGCCAACAACGCCAACCAAGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAAGAGCGCCGGCCCCAGCTGCTCAACGATCTTGGCAACAACCTCAAGGCGTTTGTCTCCCAGCG
GCTGGTGAAAACCACTGATGGCGGCCGCCGGGCTGCGGTGGAGATCATGCTGGGCACCCACACCATCCGCGACATGGTCA
AGCGCGGCGAGTTTGGCGGCCTCAAGGAGGTGATGGAGAAGTCCAAGGCCCTCGGCATGGTCACCTTCGACAGTGCCCTG
TTCGATCTGGTGGTGGAAGGGGTGATCGACGAGGAGGAGGCAGTGAAGAACGCCGATTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTATGGAAGGAGAAGGGCCAGATCGCCAGCAGCAGCGATGCCACCGGCTGGAGCCTGGAGCCCACCAAGGACG
AAAAGGACGATCTGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

53.867

98.945

0.533
  pilU Acinetobacter baylyi ADP1

52.958

93.668

0.496
  pilU Vibrio cholerae strain A1552

50

92.348

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.793

93.14

0.38
  pilT Pseudomonas aeruginosa PAK

40.407

90.765

0.367
  pilT Legionella pneumophila strain ERS1305867

41.317

88.127

0.364
  pilT Legionella pneumophila strain Lp02

41.317

88.127

0.364
  pilT Pseudomonas stutzeri DSM 10701

39.826

90.765

0.361

Multiple sequence alignment