Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AB4073_RS17520 Genome accession   NZ_CP162643
Coordinates   3725719..3727431 (+) Length   570 a.a.
NCBI ID   WP_368678136.1    Uniprot ID   -
Organism   Acinetobacter lactucae strain 128-92     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3720719..3732431
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB4073_RS17485 (AB4073_17485) nusA 3721607..3723091 (-) 1485 WP_002115134.1 transcription termination factor NusA -
  AB4073_RS17490 (AB4073_17490) rimP 3723129..3723653 (-) 525 WP_016144169.1 ribosome maturation factor RimP -
  AB4073_RS17510 (AB4073_17510) secG 3724291..3724620 (-) 330 WP_017398731.1 preprotein translocase subunit SecG -
  AB4073_RS17515 (AB4073_17515) tpiA 3724633..3725427 (-) 795 WP_368678135.1 triose-phosphate isomerase -
  AB4073_RS17520 (AB4073_17520) pilB 3725719..3727431 (+) 1713 WP_368678136.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AB4073_RS17525 (AB4073_17525) pilC 3727459..3728685 (+) 1227 WP_368678137.1 type II secretion system F family protein Machinery gene
  AB4073_RS17530 (AB4073_17530) pilD 3728685..3729545 (+) 861 WP_368678138.1 A24 family peptidase Machinery gene
  AB4073_RS17535 (AB4073_17535) coaE 3729547..3730143 (+) 597 WP_062849621.1 dephospho-CoA kinase -
  AB4073_RS17540 (AB4073_17540) - 3730140..3731054 (-) 915 WP_062849622.1 DMT family transporter -
  AB4073_RS17545 (AB4073_17545) rlmB 3731090..3731839 (-) 750 WP_171430415.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  AB4073_RS17550 (AB4073_17550) - 3731942..3732268 (-) 327 WP_002115102.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63639.07 Da        Isoelectric Point: 5.0932

>NTDB_id=1027777 AB4073_RS17520 WP_368678136.1 3725719..3727431(+) (pilB) [Acinetobacter lactucae strain 128-92]
MSVIHTSPKFSGFFRRLVEEKHVSAATMQTALDAAKRAKRDTVAYLIEEVHLSPSLLAETISIEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRVLPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKILTQQFTEESSFEFSDEEF
DLDVDLEAPPSQEDEEETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFLEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPQCKIPTDIPKQSLLEMGF
TEQDLTHPDFQVFQPVGCTECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=1027777 AB4073_RS17520 WP_368678136.1 3725719..3727431(+) (pilB) [Acinetobacter lactucae strain 128-92]
ATGTCAGTCATACATACATCTCCAAAATTTTCGGGGTTTTTTCGGCGATTAGTCGAAGAAAAACATGTGTCGGCAGCGAC
CATGCAAACAGCATTAGATGCAGCAAAGAGAGCTAAACGGGATACGGTTGCGTACCTGATTGAAGAGGTTCATCTCTCCC
CTTCTTTATTAGCTGAAACTATTTCTATTGAATTTGCTGAACCATATTTTGATCTGGACGTTTACGATACCAGCCAAATT
CCTAAAGATTTGGTTGACCAAAAACTGATTTTAAAACATCGGGTCTTACCTCTCATACAAAGAGGACAGATTTTATATGT
CGCGACCAGCAATCCAAGTAATATTGAAGCGATTGATGCCATTCGCTTCAATAGTAAGCTCATGGTTGAACCAGTTATTG
TCGAGCACCATAAGCTTGAGAAAATTCTGACGCAACAATTTACCGAAGAAAGCAGTTTCGAGTTTAGTGATGAAGAGTTT
GATCTTGATGTAGACCTTGAAGCACCACCATCACAAGAGGATGAGGAAGAAACACCCCAAGGCGATGAAGCACCTATTGT
TAAATATATTAATAAATTACTCATAGATGCGATTCGTATGGGGGCTTCAGACTTACATTTTGAACCCTATGAAAAATCAT
ATCGGGTCCGTTACCGAGTCGACGGCGTGTTGCGTCAGATTGCAAATCCGCCTTTACAATTGGCTAACCGTTTGGCTTCA
CGTTTAAAAGTCATGTCTCAAATGGATATTTCCGAAAAACGGGTGCCTCAAGATGGTCGTATCAAACTTAAGCTCTCAAA
AACTAAAGCAATCGATTTTCGTGTGAACTCACTTCCAACCTTGTTTGGTGAAAAACTGGTTCTGCGTATTCTCGATCCAT
CTAGCGCGATGCTCGGAATTGATGCTTTAGGATATGAAGAAGATCAGAAAGCTCTTTTTTTAGAAGCACTGGATAAGCCC
CAAGGTATGCTTCTAATTACAGGCCCTACTGGCTCTGGTAAAACGGTATCTTTATATACAGGCTTAAATATTTTAAATAC
TGAAAGCTCTAATATTTCCACTGCCGAAGATCCAGTCGAAATCAACCTTGAAGGTATTAATCAGGTTAATGTCAACCCGA
AAGTAGGTCTGACTTTTTCGGCCGCACTGAAGTCATTTTTACGTCAGGACCCTGACATTATTATGGTTGGTGAGATTCGT
GACTTAGAAACGGCTGAAATTGCGATTAAAGCTGCGCAAACCGGTCACATGGTAATGTCCACACTGCATACCAATAGTGC
TCCAGAAACACTAACTCGCCTACGTAATATGGGCGTTCCATCCTTTAATATCGCAACTTCTGTCAATCTGGTGATTGCTC
AACGTTTGGCGCGTCGGCTATGCCCACAGTGTAAAATACCTACCGACATTCCTAAACAGAGCCTATTAGAAATGGGCTTT
ACCGAACAAGATTTAACCCATCCCGATTTTCAAGTTTTTCAACCTGTCGGTTGTACTGAGTGTCGGGAAGGCTATAAAGG
TCGAGTAGGTATTTACGAGGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCTGCTGCTTCTGAAAAACTGGGGTTTAATAATCTGCGTCGTTCAGGATTAAAGAAAGTCATGCAAGGCGTTACTTCC
TTACAAGAAGTCAACCGCGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

95.263

100

0.953
  pilB Acinetobacter baylyi ADP1

78.246

100

0.782
  pilB Legionella pneumophila strain ERS1305867

51.675

99.474

0.514
  pilF Neisseria gonorrhoeae MS11

49.822

98.596

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

51.509

92.982

0.479
  pilB Vibrio campbellii strain DS40M4

50.958

91.579

0.467
  pilB Vibrio cholerae strain A1552

50.482

91.053

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

35.668

100

0.384
  pilF Thermus thermophilus HB27

36.833

98.596

0.363

Multiple sequence alignment