Detailed information NTG1027743

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: AB4073_RS02465
• Genome accession: NZ_CP162643
• Coordinates: 524297..525355 (+)
• Length: 352 a.a.
• NCBI ID: WP_016143662.1
• Uniprot ID: R8YYR3
• Organism: Acinetobacter lactucae strain 128-92
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 519297..530355

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB4073_RS02450 (AB4073_02450)	-	520399..520731 (+)	333	WP_368678353.1	hypothetical protein	-
AB4073_RS02455 (AB4073_02455)	-	520734..521558 (+)	825	WP_368678354.1	putative RNA methyltransferase	-
AB4073_RS02460 (AB4073_02460)	ponA	521574..524135 (-)	2562	WP_031949514.1	penicillin-binding protein PBP1a	-
AB4073_RS02465 (AB4073_02465)	pilM	524297..525355 (+)	1059	WP_016143662.1	pilus assembly protein PilM	Machinery gene
AB4073_RS02470 (AB4073_02470)	pilN	525355..525996 (+)	642	WP_016143661.1	PilN domain-containing protein	Machinery gene
AB4073_RS02475 (AB4073_02475)	pilO	525993..526772 (+)	780	WP_368678355.1	type 4a pilus biogenesis protein PilO	Machinery gene
AB4073_RS02480 (AB4073_02480)	pilP	526784..527311 (+)	528	WP_016143659.1	pilus assembly protein PilP	Machinery gene
AB4073_RS02485 (AB4073_02485)	pilQ	527374..529539 (+)	2166	WP_368678356.1	type IV pilus secretin PilQ	Machinery gene
AB4073_RS02490 (AB4073_02490)	aroK	529551..530120 (+)	570	WP_002116987.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.61 Da
• Isoelectric Point: 4.4217

>NTDB_id=1027743 AB4073_RS02465 WP_016143662.1 524297..525355(+) (pilM) [Acinetobacter lactucae strain 128-92]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALTPLPENSVVEKNILNPEAVAEALERAMNLANPQTSNAAI
AVPTSTVIHKTIEMDADMNDDEREVQIRLDAEQYIPFPLDEVSLDFEVLPDRLPNPNRVNVLLVATRTENVETRVEVLEL
VDLTPKLADVESYAVERAFSVFADSLPMGASTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
AGRAKKERSLPDDYDIEILEPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDIQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=1027743 AB4073_RS02465 WP_016143662.1 524297..525355(+) (pilM) [Acinetobacter lactucae strain 128-92]
GTGCTCAGGTTATATCGTAAACCGAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTTAAGAACGGTAAATATTGGGTGGAAAGCTATGCTTTAACACCTTTACCTGAGAACAGTGTAGTTGAAAAAAATA
TCTTGAATCCTGAAGCAGTTGCAGAAGCTTTGGAAAGGGCGATGAATTTAGCAAATCCCCAAACCAGTAATGCTGCAATT
GCTGTTCCAACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAACGATGATGAGCGTGAAGTTCAGAT
TCGATTAGATGCGGAGCAATATATACCGTTCCCTTTGGATGAGGTGAGCCTTGATTTTGAGGTTTTACCAGATCGACTGC
CAAATCCAAATCGTGTGAATGTACTTTTAGTCGCAACGCGCACAGAAAATGTTGAAACACGCGTAGAAGTACTGGAACTG
GTAGATTTAACGCCTAAACTTGCTGATGTTGAGAGTTATGCTGTCGAGCGTGCTTTTAGTGTGTTTGCTGATAGCTTACC
CATGGGTGCAAGTACCATTGGTATTTTAGATATTGGCCATACCATGACCACATTATCGGTTATGCAAAATGGCAAAATCA
TTTACACCCGTGAACAAGTCTTTGGCGGTAAACAGCTTACTCTCGAAATTCAAAGCCGTTATGGTTTGTCATTAGAAGAG
GCGGGACGGGCTAAAAAAGAACGTTCTTTGCCAGATGATTATGACATCGAAATCTTAGAACCATTTTTAGATGCTGTAGT
TCAGCAAGCTGCTAGATCTCTACAATTTTTCTTCTCTTCATCCCAATTCAATGAAATAGATCATATTTTGCTTGCAGGTG
GTAATGCCAACATTCCAGGCCTTGCGAAATTGTTGCAGCAAAAACTTGGTTACCGAGTCACTATTGCTAATCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGATATTCAAAAAATTGAAAATGATGCCTCATCATTAATGGTGGCGTGTGGCTTGGC
CTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: R8YYR3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	95.739	100	0.957
comM	Acinetobacter nosocomialis M2	95.739	100	0.957
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	41.808	100	0.42