Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   AB4073_RS02475 Genome accession   NZ_CP162643
Coordinates   525993..526772 (+) Length   259 a.a.
NCBI ID   WP_368678355.1    Uniprot ID   -
Organism   Acinetobacter lactucae strain 128-92     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 520993..531772
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB4073_RS02460 (AB4073_02460) ponA 521574..524135 (-) 2562 WP_031949514.1 penicillin-binding protein PBP1a -
  AB4073_RS02465 (AB4073_02465) pilM 524297..525355 (+) 1059 WP_016143662.1 pilus assembly protein PilM Machinery gene
  AB4073_RS02470 (AB4073_02470) pilN 525355..525996 (+) 642 WP_016143661.1 PilN domain-containing protein Machinery gene
  AB4073_RS02475 (AB4073_02475) pilO 525993..526772 (+) 780 WP_368678355.1 type 4a pilus biogenesis protein PilO Machinery gene
  AB4073_RS02480 (AB4073_02480) pilP 526784..527311 (+) 528 WP_016143659.1 pilus assembly protein PilP Machinery gene
  AB4073_RS02485 (AB4073_02485) pilQ 527374..529539 (+) 2166 WP_368678356.1 type IV pilus secretin PilQ Machinery gene
  AB4073_RS02490 (AB4073_02490) aroK 529551..530120 (+) 570 WP_002116987.1 shikimate kinase AroK -
  AB4073_RS02495 (AB4073_02495) aroB 530149..531231 (+) 1083 WP_368678357.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 259 a.a.        Molecular weight: 29280.22 Da        Isoelectric Point: 6.0348

>NTDB_id=1027745 AB4073_RS02475 WP_368678355.1 525993..526772(+) (pilO) [Acinetobacter lactucae strain 128-92]
MSPHELHELSLEQAPSKKKKITLEKFLQQFNTLDMNSYGSWPLSVKITCWIFIFFAVLALGYFVTIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQLQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
ITIEATGDYHAFGAFVSSIAALPRIVTMHDFVVEAASVKDSKSDIPVLNYSIKAKTYRYMGAGENQNNNDLQGGNGEAQA
NASDGHNVNIPSIKKSKRK

Nucleotide


Download         Length: 780 bp        

>NTDB_id=1027745 AB4073_RS02475 WP_368678355.1 525993..526772(+) (pilO) [Acinetobacter lactucae strain 128-92]
ATGAGTCCTCATGAACTTCATGAGCTGTCTTTGGAACAAGCTCCATCTAAGAAAAAAAAGATCACTTTAGAAAAATTTCT
TCAGCAATTTAATACACTCGATATGAACAGCTATGGCAGTTGGCCATTATCTGTGAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTATTGGCCTTGGGCTATTTTGTTACTATCCAGCCAAAGCTCCAAGCAATTGATAATGCTCAGGCACAAGAA
AGTAATTTATTAAATGAGTTTCGAGAAAAAGACTCAAAATTACGTAACTTGCAGCAATATCAGCTTCAACTTCAAGAAAT
GCAAGCCAACTTCAATCAGCAGTTAGAACAATTGCCAAAAGAAACCGAAATTCCAAGTTTAGTTGAAGATATTAACTTAA
CAGGAGTGAATTCTGGTCTGAAGTTTAAAAATATCCGTTTAGAAGATGAAGTGAAGCAGGAAATCTTTATTGAACAGCCG
ATTACGATTGAGGCAACAGGCGATTATCACGCTTTCGGTGCTTTCGTGAGCAGTATTGCGGCATTACCGCGTATTGTGAC
GATGCATGATTTTGTTGTTGAAGCTGCTTCTGTTAAAGATAGTAAATCAGATATTCCTGTGCTTAATTATTCTATCAAAG
CAAAAACTTATCGCTATATGGGCGCTGGGGAAAACCAAAATAATAATGACCTACAAGGGGGAAATGGTGAGGCGCAGGCC
AACGCCTCCGATGGTCATAATGTAAATATTCCTTCTATAAAGAAATCAAAGCGGAAGTAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

83.065

95.753

0.795
  comO Acinetobacter baylyi ADP1

69.959

93.822

0.656

Multiple sequence alignment