Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AB4P94_RS04065 Genome accession   NZ_CP162592
Coordinates   900986..902311 (-) Length   441 a.a.
NCBI ID   WP_395611292.1    Uniprot ID   -
Organism   Pseudomonas sp. B22129     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 895986..907311
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB4P94_RS04050 (AB4P94_04030) - 897664..899229 (-) 1566 WP_395609509.1 SPOR domain-containing protein -
  AB4P94_RS04055 (AB4P94_04035) aroB 899240..900340 (-) 1101 WP_395609510.1 3-dehydroquinate synthase -
  AB4P94_RS04060 (AB4P94_04040) aroK 900463..900981 (-) 519 WP_017256559.1 shikimate kinase AroK -
  AB4P94_RS04065 (AB4P94_04045) pilQ 900986..902311 (-) 1326 WP_395611292.1 type IV pilus secretin PilQ Machinery gene
  AB4P94_RS04070 (AB4P94_04050) - 902382..903344 (-) 963 WP_395609511.1 pilus assembly protein PilP -
  AB4P94_RS04075 (AB4P94_04055) - 903341..903892 (-) 552 WP_395609512.1 PilN domain-containing protein -
  AB4P94_RS04080 (AB4P94_04060) pilM 903889..904545 (-) 657 WP_395611293.1 type IV pilus biogenesis protein PilM -
  AB4P94_RS04085 (AB4P94_04065) - 904766..907228 (+) 2463 WP_395611294.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 47900.86 Da        Isoelectric Point: 4.7603

>NTDB_id=1027308 AB4P94_RS04065 WP_395611292.1 900986..902311(-) (pilQ) [Pseudomonas sp. B22129]
MAFAVPNRVDLIQLPPPGKAVSGAYTGDTLTLNFQNIELRAVLQQIADVAGLNLVASDEVQGSITLRLKEVPWDQALDLV
LQTKGLDKRVKAGVLLVAPADELAARELLTLEANKQMAELAPLRRELLQVNYAKAADLAKLFQSVTGLEGVTDERGSVAV
DERTNNIIAYQTGERLEELRRIVAQLDIPVRQVMIEARIVEANVDYDKNLGVRWGGRLNRGNWGAGGIGKPLAQGVEPPE
SPASSPFVDLGSLTGTSGLGLAFITDNLLLDLELTAMEKSGNGEIVSQPKVVTSDKETARILKGTEIPYQESTSQGATSV
SFKEASLSLEVTPQITPDDHVIMEVRVTKDEPDYLNKLNDVPPIKKNEVNAKVLVKDGETIVIGGVFSDTQSKVVDKVPF
LGDVPYLGRLFRRDVVAQRKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=1027308 AB4P94_RS04065 WP_395611292.1 900986..902311(-) (pilQ) [Pseudomonas sp. B22129]
ATGGCCTTTGCTGTGCCCAATCGCGTGGACCTGATTCAGCTGCCGCCTCCTGGCAAGGCAGTTTCGGGCGCGTATACCGG
CGACACACTCACCCTCAACTTCCAGAACATCGAGCTGCGCGCGGTGCTGCAGCAAATCGCTGATGTGGCGGGCCTCAACC
TGGTGGCCAGTGATGAGGTGCAGGGCTCGATCACTCTGCGGCTCAAGGAGGTGCCGTGGGACCAGGCGCTGGACCTGGTG
TTGCAAACCAAGGGGCTGGATAAACGTGTAAAAGCGGGTGTTCTGTTGGTGGCGCCGGCTGATGAGCTGGCCGCTCGTGA
GTTGCTGACACTGGAGGCAAACAAGCAGATGGCCGAGCTTGCGCCTCTGCGTCGCGAGCTGTTGCAGGTCAATTACGCCA
AGGCGGCAGACCTGGCCAAGTTGTTCCAGTCGGTGACGGGCCTTGAAGGTGTTACCGATGAGCGCGGCTCGGTGGCGGTG
GATGAGCGGACCAATAACATCATCGCCTACCAGACCGGCGAGCGGCTTGAAGAGCTGCGGCGGATTGTGGCGCAACTGGA
TATTCCGGTGCGCCAGGTGATGATCGAGGCGCGGATCGTGGAAGCCAACGTGGATTACGACAAAAACCTGGGCGTGCGCT
GGGGAGGGCGGCTCAACCGTGGCAACTGGGGCGCCGGGGGGATCGGCAAGCCCTTGGCTCAAGGTGTCGAGCCGCCGGAA
AGCCCGGCCAGCTCGCCATTTGTCGATTTGGGTTCGCTGACCGGTACTTCCGGGCTGGGCCTGGCCTTTATCACCGACAA
CCTGCTGCTGGATCTGGAGCTGACCGCCATGGAGAAGTCCGGTAACGGCGAAATCGTCTCGCAGCCCAAAGTGGTCACGT
CCGACAAGGAAACCGCGCGCATCCTCAAAGGCACCGAGATTCCGTATCAGGAATCCACCTCCCAGGGCGCTACGTCGGTG
TCGTTCAAAGAGGCCTCGCTGTCATTGGAGGTCACGCCGCAAATCACCCCCGATGACCACGTCATCATGGAGGTCCGGGT
AACCAAGGATGAGCCCGACTACCTGAATAAACTCAACGACGTGCCGCCGATCAAGAAAAACGAGGTCAACGCCAAGGTAT
TGGTCAAGGATGGCGAGACTATCGTGATCGGCGGAGTTTTCTCCGATACCCAAAGCAAAGTGGTAGATAAAGTGCCATTT
TTGGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTTGTGGCGCAGAGAAAATCCGAGCTGCTGGTGTTCCT
CACTCCGCGTATTATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(127-192)

Secretin and TonB N terminus short domain (STN)

(53-99)

Bacterial type II and III secretion system protein (Secretin)

(276-432)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.625

99.093

0.71
  pilQ Acinetobacter baumannii strain A118

39.959

100

0.447
  pilQ Acinetobacter baumannii D1279779

39.959

100

0.447
  comQ Acinetobacter baylyi ADP1

42.444

100

0.433
  pilQ Legionella pneumophila strain ERS1305867

44.762

95.238

0.426
  pilQ Vibrio campbellii strain DS40M4

44.444

95.918

0.426
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

44.391

95.011

0.422
  pilQ Vibrio cholerae strain A1552

44.391

95.011

0.422
  pilQ Legionella pneumophila strain Lp02

43.839

95.692

0.42

Multiple sequence alignment