Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AB4P96_RS29700 Genome accession   NZ_CP162589
Coordinates   6501563..6502891 (-) Length   442 a.a.
NCBI ID   WP_395601006.1    Uniprot ID   -
Organism   Pseudomonas sp. B19125     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6496563..6507891
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB4P96_RS29685 (AB4P96_29640) - 6498237..6499805 (-) 1569 WP_017138565.1 AAA family ATPase -
  AB4P96_RS29690 (AB4P96_29645) aroB 6499816..6500916 (-) 1101 WP_017138566.1 3-dehydroquinate synthase -
  AB4P96_RS29695 (AB4P96_29650) aroK 6501040..6501558 (-) 519 WP_017138567.1 shikimate kinase AroK -
  AB4P96_RS29700 (AB4P96_29655) pilQ 6501563..6502891 (-) 1329 WP_395601006.1 type IV pilus secretin PilQ Machinery gene
  AB4P96_RS29705 (AB4P96_29660) - 6502961..6503962 (-) 1002 WP_236445704.1 pilus assembly protein PilP -
  AB4P96_RS29710 (AB4P96_29665) - 6503959..6504510 (-) 552 WP_236445703.1 PilN domain-containing protein -
  AB4P96_RS29715 (AB4P96_29670) pilM 6504507..6505163 (-) 657 WP_099548046.1 type IV pilus biogenesis protein PilM -
  AB4P96_RS29720 (AB4P96_29675) - 6505383..6507845 (+) 2463 WP_395601007.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 442 a.a.        Molecular weight: 48186.23 Da        Isoelectric Point: 4.8247

>NTDB_id=1027274 AB4P96_RS29700 WP_395601006.1 6501563..6502891(-) (pilQ) [Pseudomonas sp. B19125]
MAFAVPNRVDLIQLPPPGSVVPSGAYNGDTLTLNFQNIDLRAVLQQIADVAGLNLVASDEVQGSITLRLKDVPWDQALDL
VLQTKGLDKRVKAGVLLVAPAEELAARELLALESRKQMEELAPLRRELLQVNYAKAADLAKLFQSVTGVDGFTDERGSVA
VDDRTNNIIAYQTREQIDELRRIVAQLDVPVRQVMIEARIVEANVDYDKSLGVRWGGRLNRGNWGAGGISKPLAEGAEPP
ENLPSSPFVDMGSLAGTSGLGIAFITDNLLLDLELTAMEKTGNGEIVSQPKVVTSDKETARILKGTEIPYQESSSRGATS
VSFKEASLSLEVTPQITPDGWVIMEVKVTKDEPDYLNKLNDVPPIKKNEVNAKVRVKDGETIVIGGVFSKTQSKVVDKVP
FLGDVPYLGRLFRRDVLAEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 1329 bp        

>NTDB_id=1027274 AB4P96_RS29700 WP_395601006.1 6501563..6502891(-) (pilQ) [Pseudomonas sp. B19125]
ATGGCATTTGCTGTGCCCAATCGCGTCGATCTGATCCAGCTGCCGCCTCCCGGCAGCGTGGTTCCCAGTGGCGCCTACAA
CGGCGACACGCTGACCCTCAACTTCCAGAACATCGATTTGCGCGCCGTGTTGCAGCAAATTGCGGATGTAGCCGGGCTTA
ATTTGGTGGCCAGTGATGAAGTGCAAGGCTCGATCACTTTGCGGCTCAAGGATGTGCCCTGGGACCAGGCGCTGGATCTG
GTGCTGCAAACCAAAGGCTTGGATAAGCGCGTGAAGGCCGGCGTTTTGCTGGTGGCACCCGCCGAAGAGTTGGCGGCCCG
CGAGCTGCTGGCGCTGGAGTCGCGCAAGCAGATGGAAGAGCTGGCGCCCTTGCGGCGCGAGCTGTTGCAGGTCAATTACG
CCAAGGCGGCGGACCTGGCCAAGTTGTTCCAGTCGGTGACAGGCGTTGACGGTTTTACCGATGAGCGCGGCTCGGTGGCG
GTGGATGATCGCACCAATAACATCATCGCCTACCAGACCCGCGAGCAAATTGACGAGCTGCGGCGCATCGTGGCGCAGCT
GGATGTACCGGTACGCCAAGTGATGATCGAGGCGCGGATTGTCGAGGCTAATGTCGATTACGACAAAAGCCTGGGCGTGC
GCTGGGGCGGGCGGCTCAATCGCGGCAACTGGGGCGCGGGCGGTATCAGCAAGCCGTTGGCCGAGGGTGCGGAGCCACCG
GAGAATCTGCCCAGCTCGCCGTTTGTCGATATGGGCTCGCTGGCCGGTACGTCTGGCTTGGGCATCGCTTTTATTACCGA
CAACCTGTTGCTCGACCTGGAACTGACGGCCATGGAAAAAACCGGCAACGGCGAAATCGTCTCACAGCCCAAGGTGGTCA
CTTCCGACAAGGAAACTGCGCGCATCCTCAAGGGCACCGAGATCCCTTACCAGGAATCCAGCTCGCGTGGCGCCACCTCG
GTGTCGTTCAAGGAGGCCTCGCTGTCGTTGGAGGTGACCCCGCAAATCACCCCGGATGGCTGGGTGATTATGGAGGTCAA
GGTCACCAAGGACGAGCCGGACTACCTGAACAAGCTCAACGACGTACCGCCGATCAAGAAAAACGAAGTCAACGCCAAGG
TGCGGGTCAAGGATGGCGAGACCATCGTCATTGGCGGGGTTTTCTCCAAAACGCAAAGCAAAGTGGTAGATAAAGTGCCA
TTTTTGGGCGATGTACCGTATCTTGGCCGCCTTTTCCGGCGCGATGTGCTGGCGGAGAAAAAATCCGAGCTGCTGGTATT
CCTGACTCCGCGTATTATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(54-100)

Bacterial type II/III secretion system short domain (Secretin_N)

(128-193)

Bacterial type II and III secretion system protein (Secretin)

(277-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.252

98.869

0.695
  pilQ Acinetobacter baumannii D1279779

40.891

100

0.457
  pilQ Acinetobacter baumannii strain A118

40.891

100

0.457
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

44.289

97.059

0.43
  pilQ Vibrio cholerae strain A1552

44.289

97.059

0.43
  pilQ Vibrio campbellii strain DS40M4

44.575

95.928

0.428
  comQ Acinetobacter baylyi ADP1

41.871

100

0.425
  pilQ Legionella pneumophila strain Lp02

43.571

95.023

0.414
  pilQ Legionella pneumophila strain ERS1305867

43.571

95.023

0.414

Multiple sequence alignment