Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AB4P97_RS10920 Genome accession   NZ_CP162582
Coordinates   2312019..2313128 (-) Length   369 a.a.
NCBI ID   WP_395602642.1    Uniprot ID   -
Organism   Pseudomonas sp. A1230     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2307019..2318128
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB4P97_RS10915 (AB4P97_10910) - 2308121..2311888 (-) 3768 WP_395602641.1 hypothetical protein -
  AB4P97_RS10920 (AB4P97_10915) pilU 2312019..2313128 (-) 1110 WP_395602642.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB4P97_RS10925 (AB4P97_10920) - 2313256..2315793 (+) 2538 WP_042559389.1 PAS domain-containing protein -
  AB4P97_RS10930 (AB4P97_10925) - 2315845..2316120 (+) 276 WP_025112520.1 peptidylprolyl isomerase -
  AB4P97_RS10935 (AB4P97_10930) - 2316263..2317195 (-) 933 WP_042559390.1 sugar kinase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41003.99 Da        Isoelectric Point: 7.0309

>NTDB_id=1027176 AB4P97_RS10920 WP_395602642.1 2312019..2313128(-) (pilU) [Pseudomonas sp. A1230]
MEIDALLKQLASRHGSDLFLSTGAPPSARFEGVLTPLSEQSFKPGEVATVATSLMDAEQRREFDRDLEMNLAISRPGIGR
FRVNIFKQRNDVSIVIRNVKLDIPRFEDLKLPPVLLETVMLKQGLVLFVGATDSGKSTSLAALIDHRNRHSSGHIVTIED
PIEYIHRHQRSIINQREVGVDTRSFHSALKNTLRQAPDVVLIGEIRDRETMEHALSFAETGHLVLSTLHANNANQALDRI
INMFAEERRPQLLQALGNNLKACISQRLVRTVDGNRRAAVEVLLGTPTVADLVRRCEFGELKAMMEKSAAMGMQTFDSAL
FALVSTGVITEEEALRNADSVNNLKLRLKLIGESKIDRNSTAGEWGLMD

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=1027176 AB4P97_RS10920 WP_395602642.1 2312019..2313128(-) (pilU) [Pseudomonas sp. A1230]
ATGGAAATCGATGCACTGCTCAAACAACTGGCGAGTCGTCATGGTTCGGACCTGTTCCTCTCCACGGGCGCACCGCCCAG
TGCACGTTTTGAAGGCGTGCTTACCCCGTTGAGCGAGCAATCATTCAAACCTGGCGAGGTGGCAACGGTAGCCACCTCCC
TCATGGACGCCGAGCAGCGCAGGGAGTTCGACCGGGATCTGGAAATGAACCTGGCAATCTCGCGCCCCGGCATCGGTCGC
TTCCGGGTCAACATCTTTAAACAGCGCAACGATGTATCCATTGTGATCCGCAACGTCAAACTCGATATTCCGCGTTTCGA
GGATCTCAAACTGCCGCCAGTATTGCTCGAAACCGTGATGCTCAAGCAGGGGCTGGTACTGTTTGTCGGCGCCACCGATT
CGGGAAAATCAACCTCGCTGGCAGCGCTCATCGATCACCGCAACCGCCACAGCAGCGGACATATCGTCACCATCGAAGAC
CCGATCGAGTACATCCATCGCCATCAGCGGTCGATCATCAACCAGCGTGAAGTCGGGGTCGACACTCGAAGTTTTCACTC
CGCGCTTAAAAATACCCTGCGCCAGGCGCCGGACGTGGTGCTGATCGGTGAAATTCGTGACCGTGAAACCATGGAGCACG
CGTTGTCATTTGCCGAGACAGGGCATCTGGTTTTGTCGACGTTGCACGCTAATAACGCCAATCAGGCGTTGGATCGAATT
ATCAATATGTTCGCTGAAGAGCGCCGGCCGCAGTTGCTACAGGCGCTTGGTAACAACTTGAAAGCATGTATTTCGCAGCG
TCTTGTGCGCACGGTTGATGGAAACCGCAGAGCTGCCGTAGAGGTGTTGCTGGGTACGCCGACCGTAGCCGATCTGGTCC
GACGTTGTGAGTTCGGGGAGCTCAAAGCCATGATGGAAAAATCCGCGGCTATGGGCATGCAGACATTTGACTCAGCCCTG
TTTGCGTTGGTGTCGACGGGTGTAATTACTGAGGAAGAGGCGTTAAGGAATGCCGATTCGGTCAACAATCTGAAGTTGCG
CTTGAAATTGATCGGAGAGAGCAAAATTGATCGAAACTCAACGGCGGGAGAGTGGGGTTTAATGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(78-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.056

96.206

0.539
  pilU Acinetobacter baylyi ADP1

52.528

96.477

0.507
  pilU Vibrio cholerae strain A1552

51.143

94.851

0.485
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.351

92.683

0.374
  pilT Neisseria meningitidis 8013

38.905

94.038

0.366
  pilT Neisseria gonorrhoeae MS11

38.905

94.038

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.24

90.244

0.363
  pilT Vibrio cholerae strain A1552

40.24

90.244

0.363

Multiple sequence alignment