Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   AB3471_RS04010 Genome accession   NZ_CP162520
Coordinates   930826..932043 (-) Length   405 a.a.
NCBI ID   WP_003423061.1    Uniprot ID   -
Organism   Pseudomonas syringae pv. atrofaciens strain UPB 463     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 925826..937043
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3471_RS03980 (AB3471_03975) - 927256..927789 (-) 534 WP_004415089.1 MOSC domain-containing protein -
  AB3471_RS03985 (AB3471_03980) - 927786..928415 (-) 630 WP_003345130.1 DUF1780 domain-containing protein -
  AB3471_RS03990 (AB3471_03985) - 928538..928999 (+) 462 WP_003317780.1 hypothetical protein -
  AB3471_RS03995 (AB3471_03990) yacG 929126..929335 (-) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  AB3471_RS04000 (AB3471_03995) coaE 929332..929955 (-) 624 WP_004415092.1 dephospho-CoA kinase -
  AB3471_RS04005 (AB3471_04000) pilD 929952..930824 (-) 873 WP_004415094.1 A24 family peptidase Machinery gene
  AB3471_RS04010 (AB3471_04005) pilC 930826..932043 (-) 1218 WP_003423061.1 type II secretion system F family protein Machinery gene
  AB3471_RS04015 (AB3471_04010) pilB 932046..933740 (-) 1695 WP_003317776.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AB3471_RS04020 (AB3471_04015) - 933968..934402 (+) 435 WP_003317775.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  AB3471_RS04025 (AB3471_04020) - 934631..935176 (-) 546 WP_004415097.1 DUF1523 family protein -
  AB3471_RS04030 (AB3471_04025) - 935296..936294 (+) 999 WP_004415099.1 ATP-grasp domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44167.08 Da        Isoelectric Point: 10.1809

>NTDB_id=1026877 AB3471_RS04010 WP_003423061.1 930826..932043(-) (pilC) [Pseudomonas syringae pv. atrofaciens strain UPB 463]
MASKAVKVSIYTWEGLDKKGGKLSGELSGHNPALIKAQLRKQGVNPTKVRKKSISIFGKGKKIKPLDIAFFSRQMATMMK
AGVPLLQSFDIISEGAENPNMRTLVVSLKQEVSAGNSFATALRQKPEHFDELFCNLVDAGEQAGALESLLDRVATYKEKT
EKLKAKIKKAMTYPTAVLVVAVIVSGILLIKVVPQFQTVFSSFGADLPLFTLMVIALSEFVQQWWLAILGLLFGSFFLFK
RAYKQSQKFRDSLDRFLLKVPIIGPLIFKSSVARYARTLATTFAAGVPLVEALDSVAGATGNVVFRNAVHKVKQDVSTGM
QLNFSMRSTGVFPSLAIQMTAIGEESGALDSMLDKVATYYEDEVDNMVDSLTSLMEPMIMAVLGVIVGGLVIAMYLPIFK
LGSVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1026877 AB3471_RS04010 WP_003423061.1 930826..932043(-) (pilC) [Pseudomonas syringae pv. atrofaciens strain UPB 463]
ATGGCCAGCAAGGCAGTAAAAGTCAGCATTTATACTTGGGAAGGCCTGGACAAAAAGGGTGGGAAGCTAAGTGGAGAATT
GAGCGGCCACAACCCAGCGCTTATAAAAGCGCAACTGCGCAAGCAGGGCGTCAACCCGACCAAAGTACGTAAGAAGTCAA
TTTCTATATTTGGAAAAGGTAAGAAAATCAAGCCGCTGGACATAGCGTTCTTTTCCAGGCAGATGGCCACCATGATGAAA
GCCGGTGTACCTCTACTCCAATCGTTCGACATAATCAGTGAGGGTGCCGAGAACCCCAATATGCGAACGCTGGTGGTATC
GCTAAAGCAAGAAGTCTCAGCGGGGAACAGCTTCGCGACTGCCTTGCGACAAAAACCTGAACATTTTGATGAGCTGTTCT
GCAACCTCGTTGATGCAGGTGAACAGGCAGGCGCATTGGAAAGTCTTCTAGACAGGGTAGCAACGTACAAGGAAAAAACC
GAAAAGCTCAAGGCCAAAATCAAAAAAGCCATGACCTACCCTACTGCTGTTCTCGTGGTGGCGGTAATAGTTTCAGGTAT
TCTGCTAATCAAAGTGGTACCGCAATTTCAGACAGTATTTTCAAGTTTCGGTGCAGATTTACCTCTGTTTACACTGATGG
TGATTGCCCTTTCAGAGTTTGTGCAGCAGTGGTGGCTAGCTATTCTGGGACTGCTATTTGGAAGCTTTTTTCTTTTCAAA
CGGGCCTACAAACAATCTCAGAAATTTCGCGACAGCCTAGACAGGTTCTTATTAAAAGTCCCAATCATAGGCCCGCTTAT
CTTCAAATCATCAGTAGCCCGCTATGCGCGCACTCTTGCCACGACTTTCGCTGCTGGCGTACCGCTGGTCGAAGCGCTGG
ACTCGGTGGCCGGGGCAACAGGGAATGTGGTGTTTCGAAATGCAGTCCATAAAGTAAAGCAGGATGTCTCCACCGGGATG
CAATTGAATTTCTCGATGCGCTCCACAGGTGTATTTCCAAGTTTAGCGATTCAGATGACAGCCATCGGTGAGGAATCTGG
CGCGCTCGACTCCATGCTCGATAAAGTTGCAACCTACTACGAAGATGAAGTCGATAACATGGTTGATAGCCTAACCAGCC
TCATGGAACCGATGATCATGGCGGTGCTGGGAGTTATTGTCGGCGGCCTTGTGATTGCCATGTACCTGCCCATCTTCAAA
CTTGGAAGCGTTGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.321

100

0.743
  pilC Acinetobacter baumannii D1279779

60.149

99.753

0.6
  pilC Acinetobacter baylyi ADP1

59.314

100

0.598
  pilC Legionella pneumophila strain ERS1305867

53.03

97.778

0.519
  pilC Vibrio campbellii strain DS40M4

42.26

100

0.425
  pilG Neisseria gonorrhoeae MS11

41.935

99.506

0.417
  pilG Neisseria meningitidis 44/76-A

41.687

99.506

0.415
  pilC Vibrio cholerae strain A1552

40.741

100

0.407
  pilC Thermus thermophilus HB27

39.152

99.012

0.388

Multiple sequence alignment