Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABZP31_RS01215 Genome accession   NZ_CP160437
Coordinates   261768..263477 (-) Length   569 a.a.
NCBI ID   WP_034672272.1    Uniprot ID   A0A3D3FZM5
Organism   Acinetobacter sp. FL     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 256768..268477
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABZP31_RS01185 (ABZP31_01185) - 256900..257223 (+) 324 WP_005016998.1 pyrimidine/purine nucleoside phosphorylase -
  ABZP31_RS01190 (ABZP31_01190) rlmB 257327..258082 (+) 756 WP_005405959.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ABZP31_RS01195 (ABZP31_01195) - 258107..259042 (+) 936 WP_101235097.1 DMT family transporter -
  ABZP31_RS01200 (ABZP31_01200) coaE 259039..259650 (-) 612 WP_005016991.1 dephospho-CoA kinase -
  ABZP31_RS01205 (ABZP31_01205) pilD 259652..260512 (-) 861 WP_248134768.1 A24 family peptidase Machinery gene
  ABZP31_RS01210 (ABZP31_01210) pilC 260512..261738 (-) 1227 WP_248134770.1 type II secretion system F family protein Machinery gene
  ABZP31_RS01215 (ABZP31_01215) pilB 261768..263477 (-) 1710 WP_034672272.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABZP31_RS01220 (ABZP31_01220) tpiA 263766..264560 (+) 795 WP_034672273.1 triose-phosphate isomerase -
  ABZP31_RS01225 (ABZP31_01225) secG 264571..264900 (+) 330 WP_017400668.1 preprotein translocase subunit SecG -
  ABZP31_RS01245 (ABZP31_01245) rimP 265527..266051 (+) 525 WP_005016981.1 ribosome maturation factor RimP -
  ABZP31_RS01250 (ABZP31_01250) nusA 266092..267576 (+) 1485 WP_248132750.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63611.86 Da        Isoelectric Point: 5.0245

>NTDB_id=1021116 ABZP31_RS01215 WP_034672272.1 261768..263477(-) (pilB) [Acinetobacter sp. FL]
MTVNFTPKFSGFIRRLVEDNHVSAAEMQNAVDTAKRNRQDIVPYLIDHFKLSPLLIAEFISIEFGEALFDLAVYDTVQIP
RDVVDEKLILKHRVLPLIKRGHTLFVATSNPTNIEALEAVRFNSKLNIEVVIVEHDKLQRTIEQNFSEESNFDFGDEELN
IDVSTSNLDEDDSQDNAPQEEEAPIVKYINKLLVDAIRIGASDLHFEPYEKYYRVRYRVDGVLRQVATPPLQLATRLASR
LKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPEQKALFIEALEKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNNKVGLSFSAALKSFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVAAFNIATSVNLVIAQRLARRLCNQCKVPAEIPRNSLLEMGFT
EDDLANPDFQVYQPVGCPECRDGYRGRVGIYEVMKVTPEISKIIMEDGNALEIAATAARLGFDNLRRSGLKKVMQGVTSL
QEINRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=1021116 ABZP31_RS01215 WP_034672272.1 261768..263477(-) (pilB) [Acinetobacter sp. FL]
ATGACAGTTAATTTTACTCCAAAGTTTTCCGGTTTTATTCGCCGTCTGGTTGAGGATAACCACGTGTCTGCTGCTGAAAT
GCAGAATGCGGTCGATACTGCTAAAAGAAACCGGCAGGATATCGTGCCTTATCTGATCGATCATTTTAAGCTTTCACCCT
TACTGATTGCAGAATTTATCTCGATTGAATTTGGTGAAGCTTTATTCGATTTGGCTGTTTACGATACTGTCCAGATTCCA
CGAGATGTAGTTGATGAGAAACTGATACTCAAGCATCGGGTCTTGCCCCTCATTAAGCGTGGTCATACTCTGTTTGTTGC
TACCAGTAATCCCACCAATATTGAAGCTTTAGAAGCTGTCCGGTTTAACAGTAAACTGAATATTGAAGTTGTTATTGTCG
AGCATGACAAACTGCAGCGTACTATTGAGCAGAATTTTTCTGAAGAAAGCAATTTTGATTTTGGTGATGAAGAACTCAAT
ATTGATGTTTCAACCAGCAATTTAGATGAGGACGACAGTCAAGATAATGCGCCTCAGGAAGAAGAAGCTCCAATTGTTAA
ATATATTAACAAGTTATTGGTAGATGCCATTCGTATTGGTGCATCAGACTTACATTTCGAGCCTTATGAAAAATATTACC
GGGTACGTTACCGGGTCGATGGCGTGTTAAGACAGGTGGCAACACCACCTTTACAGCTGGCCACCCGACTGGCTTCACGT
TTAAAGGTTATGTCACAGATGGATATTTCCGAAAAGCGCGTTCCCCAGGACGGCCGGATCAAATTAAAACTCTCTAAGAC
CAAAGCTATTGATTTCCGTGTGAACTCACTGCCTACACTATTTGGAGAAAAACTGGTTCTGCGTATTTTGGATCCGTCCA
GCGCCATGCTGGGTATTGATGCATTAGGCTATGAACCCGAGCAAAAAGCCCTGTTTATAGAAGCTTTAGAAAAGCCGCAA
GGTATGTTACTCATTACCGGGCCTACCGGTTCCGGTAAAACCGTATCGCTTTATACAGGCCTCAATATTTTAAACCGTGA
GGATACCAATATTTCAACTGCCGAAGACCCGGTAGAAATTAATCTTGAAGGTATTAACCAGGTCAATGTAAATAATAAAG
TCGGGTTAAGCTTCTCTGCCGCGCTAAAGTCATTCCTGCGTCAGGATCCAGATATTATTATGGTAGGTGAGATTCGTGAC
TTGGAAACAGCAGAGATTGCCATTAAAGCAGCTCAGACCGGTCACATGGTTATGTCTACCCTGCATACCAACAGCGCTCC
TGAAACATTAACACGCCTAAGAAATATGGGCGTAGCAGCATTTAATATTGCCACTTCAGTTAATCTGGTCATTGCACAGC
GTCTAGCTCGACGTTTATGCAACCAGTGCAAGGTACCAGCAGAGATTCCTAGAAATAGTCTGCTTGAGATGGGCTTTACC
GAAGATGACTTGGCCAACCCTGATTTTCAGGTTTATCAACCTGTCGGTTGCCCGGAATGTCGTGATGGTTATCGGGGTCG
TGTGGGTATCTATGAAGTAATGAAAGTTACACCGGAAATTTCAAAAATTATTATGGAAGACGGTAACGCCCTTGAAATAG
CAGCAACAGCAGCACGCCTGGGTTTCGATAACTTGCGTCGTTCTGGCCTGAAGAAAGTCATGCAAGGTGTAACCTCTTTA
CAAGAAATTAACCGTGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(56-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3D3FZM5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.206

99.121

0.805
  pilB Acinetobacter baylyi ADP1

78.153

98.946

0.773
  pilB Legionella pneumophila strain ERS1305867

53.493

95.606

0.511
  pilF Neisseria gonorrhoeae MS11

49.644

98.77

0.49
  pilB Vibrio parahaemolyticus RIMD 2210633

51.718

92.091

0.476
  pilB Vibrio cholerae strain A1552

50.478

91.916

0.464
  pilB Vibrio campbellii strain DS40M4

50.287

91.916

0.462
  pilB/pilB1 Synechocystis sp. PCC 6803

34.355

100

0.374
  pilF Thermus thermophilus HB27

36.702

99.121

0.364

Multiple sequence alignment