Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ABZP20_RS02530 Genome accession   NZ_CP160433
Coordinates   541743..542252 (+) Length   169 a.a.
NCBI ID   WP_012434937.1    Uniprot ID   A0AAD2BNJ9
Organism   Ralstonia sp. CP     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 536743..547252
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABZP20_RS02505 (ABZP20_02505) - 537105..537731 (+) 627 WP_012434932.1 DUF2889 domain-containing protein -
  ABZP20_RS02510 (ABZP20_02510) sucC 537798..538964 (+) 1167 WP_012434933.1 ADP-forming succinate--CoA ligase subunit beta -
  ABZP20_RS02515 (ABZP20_02515) sucD 539053..539934 (+) 882 WP_012434934.1 succinate--CoA ligase subunit alpha -
  ABZP20_RS02520 (ABZP20_02520) - 540088..540783 (+) 696 WP_012434935.1 TerC family protein -
  ABZP20_RS02525 (ABZP20_02525) pilA 540969..541490 (+) 522 WP_012434936.1 pilin Machinery gene
  ABZP20_RS02530 (ABZP20_02530) pilA 541743..542252 (+) 510 WP_012434937.1 pilin Machinery gene
  ABZP20_RS02535 (ABZP20_02535) - 542324..544060 (+) 1737 WP_012434938.1 O-antigen ligase family protein -
  ABZP20_RS02540 (ABZP20_02540) pilA 544247..544771 (+) 525 WP_012434939.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  ABZP20_RS02545 (ABZP20_02545) moaC 544818..545294 (-) 477 WP_012434940.1 cyclic pyranopterin monophosphate synthase MoaC -
  ABZP20_RS02550 (ABZP20_02550) - 545400..547109 (+) 1710 WP_012434941.1 M48 family metalloprotease -

Sequence

Protein


Download         Length: 169 a.a.        Molecular weight: 17396.00 Da        Isoelectric Point: 10.0707

>NTDB_id=1021088 ABZP20_RS02530 WP_012434937.1 541743..542252(+) (pilA) [Ralstonia sp. CP]
MKSMRRLNKRVQKGFTLIELMIVVAIVGILAAIAIPAYQDYTIRARVTEGLSLAAAAKVLVAENAANAQSDLSAGSTVFA
ATKNVNNLVVSGTDGHITITYGSAVSTSNPTLTLTPYFTVGSTNSNLAQSSAPAAPVQWKCASASATAPTGVTVTLGSLP
AKWAPAECR

Nucleotide


Download         Length: 510 bp        

>NTDB_id=1021088 ABZP20_RS02530 WP_012434937.1 541743..542252(+) (pilA) [Ralstonia sp. CP]
ATGAAATCGATGCGTCGTCTGAACAAGCGGGTCCAGAAGGGCTTCACGCTGATCGAACTGATGATCGTGGTTGCAATCGT
CGGTATTCTGGCTGCTATCGCCATTCCGGCGTATCAGGACTACACGATTCGTGCCCGCGTGACGGAAGGTCTGTCGCTGG
CTGCTGCTGCCAAGGTGTTGGTGGCCGAAAACGCAGCCAATGCACAAAGCGATCTGTCGGCTGGTTCCACGGTGTTTGCG
GCTACGAAAAACGTGAATAACTTGGTCGTCTCGGGTACGGATGGGCATATCACGATTACCTATGGCTCGGCCGTGTCCAC
CTCTAACCCGACGCTGACGCTCACGCCGTATTTCACGGTTGGCTCGACGAACTCCAACCTGGCTCAAAGCTCCGCACCGG
CCGCTCCGGTTCAGTGGAAGTGCGCATCGGCAAGCGCAACGGCGCCTACCGGCGTGACTGTGACGCTTGGCTCGCTGCCG
GCCAAGTGGGCACCGGCTGAGTGCCGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-34)

Pilin (bacterial filament) (Pilin)

(44-169)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

63.483

100

0.669
  pilA2 Legionella pneumophila strain ERS1305867

47.531

95.858

0.456
  pilA2 Legionella pneumophila str. Paris

47.531

95.858

0.456
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

35.025

100

0.408
  comP Acinetobacter baylyi ADP1

38.953

100

0.396

Multiple sequence alignment