Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ABZP20_RS02540 Genome accession   NZ_CP160433
Coordinates   544247..544771 (+) Length   174 a.a.
NCBI ID   WP_012434939.1    Uniprot ID   A0AAD2BQD9
Organism   Ralstonia sp. CP     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 539247..549771
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABZP20_RS02520 (ABZP20_02520) - 540088..540783 (+) 696 WP_012434935.1 TerC family protein -
  ABZP20_RS02525 (ABZP20_02525) pilA 540969..541490 (+) 522 WP_012434936.1 pilin Machinery gene
  ABZP20_RS02530 (ABZP20_02530) pilA 541743..542252 (+) 510 WP_012434937.1 pilin Machinery gene
  ABZP20_RS02535 (ABZP20_02535) - 542324..544060 (+) 1737 WP_012434938.1 O-antigen ligase family protein -
  ABZP20_RS02540 (ABZP20_02540) pilA 544247..544771 (+) 525 WP_012434939.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  ABZP20_RS02545 (ABZP20_02545) moaC 544818..545294 (-) 477 WP_012434940.1 cyclic pyranopterin monophosphate synthase MoaC -
  ABZP20_RS02550 (ABZP20_02550) - 545400..547109 (+) 1710 WP_012434941.1 M48 family metalloprotease -
  ABZP20_RS02555 (ABZP20_02555) - 547130..547711 (-) 582 WP_012434942.1 DUF2946 family protein -
  ABZP20_RS02560 (ABZP20_02560) - 547704..548780 (-) 1077 WP_012434943.1 alpha/beta fold hydrolase -
  ABZP20_RS02565 (ABZP20_02565) - 548815..549255 (-) 441 WP_009239042.1 nuclear transport factor 2 family protein -

Sequence

Protein


Download         Length: 174 a.a.        Molecular weight: 18483.03 Da        Isoelectric Point: 7.8339

>NTDB_id=1021089 ABZP20_RS02540 WP_012434939.1 544247..544771(+) (pilA) [Ralstonia sp. CP]
MQLHYQYNGRHQRGFTLIELMIVVAIVGILAAIAVPAYQDYVTRARVSEGLALAASAKTTVAENASAGVPFDEGFAADTK
ATRYVQAKGIVISQKTGEIQIKYTDSVAKDGENVLLLKPTTYDEAMSGSSASSVRPTGPIRWDCYAKGAAVRAGTAELET
KPSLATNVAPAECR

Nucleotide


Download         Length: 525 bp        

>NTDB_id=1021089 ABZP20_RS02540 WP_012434939.1 544247..544771(+) (pilA) [Ralstonia sp. CP]
ATGCAGTTGCATTACCAGTACAACGGGCGACACCAGCGCGGTTTTACTCTGATTGAGCTGATGATCGTTGTTGCGATTGT
CGGTATTCTGGCGGCGATCGCCGTCCCCGCGTATCAAGATTACGTCACGCGCGCCCGCGTAAGCGAGGGGTTGGCCCTCG
CAGCATCAGCCAAGACAACTGTGGCCGAGAACGCCAGCGCTGGGGTTCCGTTCGACGAGGGTTTCGCCGCAGACACGAAA
GCCACGCGCTACGTCCAAGCGAAAGGCATCGTGATCAGTCAAAAGACTGGCGAAATTCAAATTAAATACACTGATTCGGT
AGCGAAAGACGGTGAAAATGTCTTGTTGCTCAAGCCAACAACCTATGATGAAGCCATGAGCGGGTCAAGCGCGAGCAGTG
TTCGCCCCACAGGTCCAATTCGTTGGGATTGCTATGCCAAGGGCGCCGCCGTCCGAGCAGGAACAGCCGAACTTGAAACG
AAGCCGTCATTGGCGACAAATGTTGCACCTGCGGAATGCCGGTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(43-174)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

46.927

100

0.483
  pilA2 Legionella pneumophila strain ERS1305867

39.412

97.701

0.385

Multiple sequence alignment