Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AB0A83_RS19545 Genome accession   NZ_CP160405
Coordinates   4325190..4326902 (-) Length   570 a.a.
NCBI ID   WP_150051381.1    Uniprot ID   -
Organism   Methylomonas sp. HYX-M1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4320190..4331902
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB0A83_RS19515 (AB0A83_19515) - 4320498..4321469 (+) 972 WP_367154999.1 Nudix family hydrolase -
  AB0A83_RS19520 (AB0A83_19520) yacG 4321441..4321644 (-) 204 WP_150051371.1 DNA gyrase inhibitor YacG -
  AB0A83_RS19525 (AB0A83_19525) zapD 4321647..4322417 (-) 771 WP_150051373.1 cell division protein ZapD -
  AB0A83_RS19530 (AB0A83_19530) coaE 4322414..4323025 (-) 612 WP_150051375.1 dephospho-CoA kinase -
  AB0A83_RS19535 (AB0A83_19535) pilD 4323027..4323893 (-) 867 WP_367155000.1 A24 family peptidase Machinery gene
  AB0A83_RS19540 (AB0A83_19540) pilC 4323966..4325186 (-) 1221 WP_150051379.1 type II secretion system F family protein Machinery gene
  AB0A83_RS19545 (AB0A83_19545) pilB 4325190..4326902 (-) 1713 WP_150051381.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AB0A83_RS19550 (AB0A83_19550) - 4327001..4327795 (-) 795 WP_150051383.1 response regulator -
  AB0A83_RS19555 (AB0A83_19555) - 4327802..4329244 (-) 1443 WP_367155001.1 HDOD domain-containing protein -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62725.32 Da        Isoelectric Point: 5.8902

>NTDB_id=1021012 AB0A83_RS19545 WP_150051381.1 4325190..4326902(-) (pilB) [Methylomonas sp. HYX-M1]
MVTAPPNIHFSGLAKCLVQQGLLTEDAAAVAAQEAQKQKQPLVTYLVANKQVDSRTIAKLASSEFGVPLLDLDAVDLTSI
PVKLVSEKLIRQHHALPLFKRGNRLFIATADPTNFQALDEIKFHTRLNTENILVEEDKLVRCIDAALEAVDTTMGNLLDE
DLDNIDISGGDEKPAEDVSSSDIDEAPIVRFVNKILLDSIKKGVSDIHLEPFEKRFRIRFRADGILAEIASPPIGIANRI
ISRIKVMSKMDIAERRVPQDGRIKMQLSKTRAIDFRVNTCPTLFGEKVVLRILDPTSAQIGIEKLGFEPAQQKYFLDAIH
KPYGMVLVTGPTGSGKTVSLYTGLNLLNSVERNISTAEDPVEITVEGINQVNVNPKAGLTFAEALKAFLRQDPDVIMVGE
IRDIETASIAVKAAQTGHLVLSTLHTNDAPQTINRLVQMGIEPFNIVSAVNLIMAQRLARRLCEHCKKEADFPANVWLAA
GFNEEEIGTFKLYEPVGCEHCNNGYKGRVGIYQVLPISEKMRALILRGGNSMEMAELAKSEGINDLRASGLLKVRQGITS
LEEVDRVTKD

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=1021012 AB0A83_RS19545 WP_150051381.1 4325190..4326902(-) (pilB) [Methylomonas sp. HYX-M1]
ATGGTGACGGCTCCTCCTAACATCCATTTTAGTGGCTTAGCAAAATGTTTAGTCCAACAAGGCTTGCTAACCGAAGACGC
TGCAGCTGTAGCCGCACAAGAGGCCCAAAAACAAAAACAACCACTGGTAACCTATCTGGTTGCCAACAAGCAAGTCGATA
GTCGAACTATCGCCAAATTAGCGTCTAGCGAATTTGGGGTGCCACTGCTGGATCTGGACGCAGTTGACCTCACGTCGATT
CCGGTCAAATTAGTCAGCGAAAAACTAATTCGTCAACATCATGCGTTACCTCTATTCAAACGCGGTAACCGTTTGTTTAT
TGCAACTGCAGATCCTACAAATTTCCAGGCTCTGGATGAAATCAAGTTTCATACGCGGTTAAATACCGAAAATATTCTCG
TCGAAGAAGATAAGTTAGTCCGTTGTATCGATGCTGCTTTGGAAGCGGTTGATACGACAATGGGGAATTTACTTGACGAA
GATTTAGATAATATCGATATTTCCGGCGGCGATGAAAAGCCGGCGGAAGACGTATCGTCTTCCGACATCGACGAGGCGCC
GATCGTTCGTTTCGTCAATAAAATTCTATTGGATTCGATAAAAAAAGGGGTTTCCGACATCCACTTAGAGCCGTTCGAAA
AGCGATTCAGGATTCGATTTCGCGCGGACGGTATCCTTGCTGAAATCGCAAGCCCACCAATCGGGATCGCTAACCGAATT
ATTTCCCGCATCAAAGTCATGTCGAAAATGGATATTGCAGAACGCCGTGTGCCGCAGGACGGGCGGATCAAAATGCAATT
ATCGAAGACGCGAGCCATTGATTTTCGGGTAAATACCTGTCCGACTTTGTTTGGCGAAAAGGTTGTATTGAGGATCCTCG
ATCCTACCAGTGCGCAAATCGGGATAGAAAAATTGGGCTTTGAACCCGCGCAACAAAAATATTTTTTAGACGCCATACAT
AAGCCATATGGAATGGTTTTGGTTACAGGGCCCACCGGTAGTGGTAAGACGGTTTCCTTGTATACGGGGCTAAACTTGCT
GAATAGCGTCGAACGAAATATTTCCACTGCAGAAGATCCGGTCGAAATTACAGTGGAGGGTATTAATCAGGTAAACGTCA
ATCCCAAGGCAGGACTAACTTTTGCCGAAGCGCTAAAAGCATTTTTACGTCAAGACCCGGACGTTATAATGGTGGGCGAA
ATTAGGGATATAGAGACTGCAAGTATTGCGGTAAAAGCCGCGCAAACGGGACACTTAGTGTTGTCCACTCTGCACACCAA
CGATGCTCCGCAGACAATCAACCGCTTGGTGCAAATGGGCATAGAGCCGTTCAATATCGTATCCGCCGTCAATTTAATTA
TGGCACAACGTTTAGCGAGAAGGTTGTGCGAACATTGCAAAAAGGAAGCCGATTTTCCTGCAAATGTTTGGTTAGCGGCT
GGTTTTAACGAGGAGGAGATCGGTACATTCAAATTATACGAGCCGGTCGGTTGCGAACATTGTAACAACGGGTATAAAGG
TAGGGTGGGGATTTACCAAGTGTTGCCCATCAGTGAAAAAATGCGGGCGTTAATTCTTCGCGGCGGAAATTCGATGGAGA
TGGCTGAATTGGCAAAATCCGAAGGTATCAACGATTTACGGGCTTCCGGTTTGTTGAAAGTGCGGCAAGGTATTACCTCG
CTGGAAGAAGTCGACCGCGTAACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

55.575

100

0.56
  pilB Acinetobacter baylyi ADP1

53.415

100

0.535
  pilB Acinetobacter baumannii D1279779

52.66

98.947

0.521
  pilF Neisseria gonorrhoeae MS11

47.695

98.947

0.472
  pilB Vibrio parahaemolyticus RIMD 2210633

47.153

98.596

0.465
  pilB Vibrio campbellii strain DS40M4

46.359

98.772

0.458
  pilB Vibrio cholerae strain A1552

50.196

89.474

0.449
  pilF Thermus thermophilus HB27

39.892

97.193

0.388
  pilB/pilB1 Synechocystis sp. PCC 6803

40.927

90.877

0.372
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.993

97.895

0.372

Multiple sequence alignment