Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABV589_RS27140 Genome accession   NZ_CP160398
Coordinates   6043239..6044351 (+) Length   370 a.a.
NCBI ID   WP_367084375.1    Uniprot ID   -
Organism   Pseudomonas sp. HOU2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6038239..6049351
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABV589_RS27125 (ABV589_27125) - 6039182..6040117 (+) 936 WP_367084373.1 sugar kinase -
  ABV589_RS27130 (ABV589_27130) - 6040241..6040516 (-) 276 WP_003225447.1 peptidylprolyl isomerase -
  ABV589_RS27135 (ABV589_27135) - 6040556..6043105 (-) 2550 WP_367084374.1 PAS domain-containing protein -
  ABV589_RS27140 (ABV589_27140) pilU 6043239..6044351 (+) 1113 WP_367084375.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABV589_RS27145 (ABV589_27145) - 6044464..6046419 (-) 1956 WP_367084376.1 acetoacetate--CoA ligase -
  ABV589_RS27150 (ABV589_27150) hbdH 6046587..6047360 (-) 774 WP_047294787.1 3-hydroxybutyrate dehydrogenase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41068.88 Da        Isoelectric Point: 6.6254

>NTDB_id=1020878 ABV589_RS27140 WP_367084375.1 6043239..6044351(+) (pilU) [Pseudomonas sp. HOU2]
MEIDALLLRLSSQHGSDLFLSTGAPPSARIDGVLTPFSERPFKPGEVEAIANSLMDAEQRREFDRDLEMNLAISRTGVGR
FRVNIFKQRNDVSIVIRNVKLDIPRFEDLKLPPVLLETVMLKQGLILFVGATDSGKSTSLAALIDHRNRHSSGHIITIED
PVEYIHRHQRSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALTFADTGHLVLSTLHAHNANQALDRI
VNLFPEERRPQLLHALGNNLKAFVSQRLVRTVDGQRRAAVEVMLGTPTIADLIRRNELGELKGIMEKSGEVGMQTFDAAL
YGLVVEGVISEDEALKHADSQNNLRLRLKLHAEAGPAPAPSSSGDWGLMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=1020878 ABV589_RS27140 WP_367084375.1 6043239..6044351(+) (pilU) [Pseudomonas sp. HOU2]
ATGGAAATCGATGCACTGTTGCTCCGTCTGTCGAGCCAGCACGGCTCTGACCTGTTCCTGTCCACGGGAGCGCCACCCAG
TGCGCGCATCGATGGCGTGCTGACGCCGTTCAGCGAGCGGCCGTTCAAGCCCGGTGAGGTCGAGGCCATCGCCAACTCCT
TGATGGACGCCGAGCAGCGGCGCGAGTTCGATCGGGATCTGGAAATGAACCTGGCGATTTCCCGTACGGGCGTCGGGCGT
TTTCGGGTGAACATCTTCAAGCAACGCAATGATGTGTCAATCGTGATCCGCAACGTCAAACTCGATATCCCGCGATTCGA
AGACCTGAAACTGCCGCCGGTACTGCTGGAAACGGTGATGCTCAAACAGGGGCTGATCCTGTTCGTTGGCGCCACCGACT
CCGGTAAATCCACCTCGCTGGCAGCGCTGATTGACCACCGCAACCGCCACAGCAGCGGGCACATCATCACCATCGAAGAC
CCGGTGGAGTATATCCATCGGCACCAGCGCTCGATCATCAATCAACGCGAAGTCGGCGTCGATACCCGCAGTTTCCATGC
GGCGCTGAAAAACACCCTGCGCCAGGCCCCGGACGTGGTGCTGATCGGCGAGATCCGCGACCGCGAAACCATGGAGCATG
CGCTGACGTTTGCCGACACCGGGCACCTGGTGCTGTCGACGCTGCATGCGCACAACGCCAATCAGGCGCTGGACCGTATC
GTCAATCTGTTCCCCGAAGAGCGCCGCCCGCAGTTGCTGCATGCGCTGGGCAACAATCTCAAGGCCTTTGTTTCGCAACG
GTTGGTGCGCACTGTTGATGGGCAGCGCCGGGCGGCGGTCGAGGTGATGCTTGGCACCCCGACCATCGCCGACCTGATCC
GCCGCAACGAGTTGGGCGAACTCAAGGGCATCATGGAGAAGTCCGGCGAGGTCGGCATGCAGACCTTTGACGCGGCGCTG
TACGGGCTGGTGGTGGAGGGCGTGATCAGTGAAGACGAAGCGCTCAAACATGCCGATTCGCAGAACAATCTGCGCCTGCG
TTTGAAACTGCACGCCGAGGCGGGCCCGGCGCCCGCGCCTTCATCATCAGGCGATTGGGGCTTGATGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(68-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.338

95.946

0.541
  pilU Acinetobacter baylyi ADP1

53.221

96.486

0.514
  pilU Vibrio cholerae strain A1552

53.143

94.595

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.005

100

0.403
  pilT Pseudomonas stutzeri DSM 10701

40.407

92.973

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.173

93.514

0.376
  pilT Vibrio cholerae strain A1552

40.173

93.514

0.376
  pilT Pseudomonas aeruginosa PAK

39.826

92.973

0.37

Multiple sequence alignment