Detailed information NTG1018034

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: ABZS48_RS02295
• Genome accession: NZ_CP160100
• Coordinates: 426583..427080 (+)
• Length: 165 a.a.
• NCBI ID: WP_001796472.1
• Uniprot ID: A0A5F0HJ55
• Organism: Staphylococcus aureus strain A_01_sau
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 421583..432080

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABZS48_RS02255 (ABZS48_02255)	-	421627..422613 (+)	987	WP_000161307.1	ROK family glucokinase	-
ABZS48_RS02260 (ABZS48_02260)	-	422613..422942 (+)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
ABZS48_RS02265 (ABZS48_02265)	-	422939..423562 (+)	624	WP_001223009.1	MBL fold metallo-hydrolase	-
ABZS48_RS02270 (ABZS48_02270)	comGA	423614..424588 (+)	975	WP_000697220.1	competence type IV pilus ATPase ComGA	Machinery gene
ABZS48_RS02275 (ABZS48_02275)	comGB	424560..425630 (+)	1071	WP_000776409.1	competence type IV pilus assembly protein ComGB	Machinery gene
ABZS48_RS02280 (ABZS48_02280)	comGC	425644..425955 (+)	312	WP_000472255.1	competence type IV pilus major pilin ComGC	Machinery gene
ABZS48_RS02285 (ABZS48_02285)	comGD	425933..426379 (+)	447	WP_001796473.1	competence type IV pilus minor pilin ComGD	Machinery gene
ABZS48_RS02290 (ABZS48_02290)	comGE	426366..426665 (+)	300	WP_000844413.1	hypothetical protein	Machinery gene
ABZS48_RS02295 (ABZS48_02295)	comGF	426583..427080 (+)	498	WP_001796472.1	competence type IV pilus minor pilin ComGF	Machinery gene
ABZS48_RS02300 (ABZS48_02300)	-	427177..427323 (+)	147	WP_001789879.1	hypothetical protein	-
ABZS48_RS02305 (ABZS48_02305)	-	427313..427837 (+)	525	WP_033858309.1	shikimate kinase	-
ABZS48_RS02310 (ABZS48_02310)	gcvT	427996..429087 (+)	1092	WP_000093344.1	glycine cleavage system aminomethyltransferase GcvT	-
ABZS48_RS02315 (ABZS48_02315)	gcvPA	429107..430453 (+)	1347	WP_412103827.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
ABZS48_RS02320 (ABZS48_02320)	gcvPB	430446..431918 (+)	1473	WP_000202176.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19169.89 Da
• Isoelectric Point: 10.1358

>NTDB_id=1018034 ABZS48_RS02295 WP_001796472.1 426583..427080(+) (comGF) [Staphylococcus aureus strain A_01_sau]
MILSKVTNKYVPFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide

• Length: 498 bp

>NTDB_id=1018034 ABZS48_RS02295 WP_001796472.1 426583..427080(+) (comGF) [Staphylococcus aureus strain A_01_sau]
ATGATATTAAGCAAAGTGACCAACAAATATGTGCCATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACAATTTATGTATAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (68-155)

• Prokaryotic N-terminal methylation motif (N_methyl) (28-51)

3D structure

• AlphaFold DB: A0A5F0HJ55

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus N315	98.182	100	0.982
comGF	Staphylococcus aureus MW2	95.758	100	0.958