Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABZM26_RS11735 Genome accession   NZ_CP160044
Coordinates   2652157..2653266 (+) Length   369 a.a.
NCBI ID   WP_016774502.1    Uniprot ID   A0AAQ2HZW3
Organism   Pseudomonas atacamensis strain GZ-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2647157..2658266
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABZM26_RS11720 - 2648145..2649065 (+) 921 WP_366301966.1 sugar kinase -
  ABZM26_RS11725 - 2649182..2649457 (-) 276 WP_123442629.1 peptidylprolyl isomerase -
  ABZM26_RS11730 - 2649498..2652047 (-) 2550 WP_366301968.1 PAS domain-containing protein -
  ABZM26_RS11735 pilU 2652157..2653266 (+) 1110 WP_016774502.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABZM26_RS11740 - 2653401..2657159 (+) 3759 WP_366301970.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40841.77 Da        Isoelectric Point: 7.0374

>NTDB_id=1017280 ABZM26_RS11735 WP_016774502.1 2652157..2653266(+) (pilU) [Pseudomonas atacamensis strain GZ-3]
MEIDALLSILSQKNGSDLFLSTGAAPSARIDGVLTALSDQPFKPGETAAIATSLMDAEQRREFDRDLEMNLAISRTGVGR
FRVNIFKQRNDVSIVIRNVKVDIPRFEDLKLPPVLLETVMLKRGLILFVGATSSGKSTSLAALIDHRNRHSAGHIVTIED
PIEYIHRHKRSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALSFADTGHLVLSTLHATNANQALDRI
INMFPEEKREQLLQTLGNNLKACVSQRLVRTVDGQRRAAVEILLGTPTIADLIRRGQFEELKPMMEKSTGLGMQTFDAAL
FALFAEGAISEQEALKNADSVNNLKLRIKLQHDQAGVNNTGTGEWGLMD

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=1017280 ABZM26_RS11735 WP_016774502.1 2652157..2653266(+) (pilU) [Pseudomonas atacamensis strain GZ-3]
ATGGAAATCGACGCACTGTTGTCAATATTGTCGCAAAAAAACGGCTCGGATCTGTTCCTCTCTACCGGCGCAGCGCCGAG
CGCGCGCATTGATGGCGTGCTGACGGCGCTCAGCGATCAGCCCTTCAAGCCCGGCGAAACCGCGGCCATCGCCACCTCCC
TGATGGACGCCGAGCAGCGCCGGGAGTTCGACCGGGATCTGGAAATGAACCTGGCAATCTCCCGAACGGGCGTCGGGCGG
TTCCGGGTGAACATTTTCAAGCAGCGCAACGACGTTTCGATCGTGATCCGCAACGTCAAGGTCGACATCCCGCGATTCGA
AGACCTGAAGCTGCCGCCGGTGCTGCTGGAAACGGTGATGCTCAAACGTGGCCTGATTCTTTTTGTCGGCGCGACCAGTT
CAGGCAAGTCGACTTCACTGGCGGCGCTCATCGATCACCGTAACCGCCATAGCGCCGGACACATCGTGACGATCGAAGAC
CCGATCGAATATATCCATCGACACAAGCGCTCGATCATCAATCAGCGTGAAGTCGGCGTAGATACCCGAAGCTTTCATGC
GGCTTTGAAGAACACTCTGCGCCAGGCTCCGGATGTTGTATTGATTGGTGAGATCCGTGACCGCGAAACCATGGAGCATG
CGCTGTCATTCGCTGACACCGGCCATCTTGTGTTGTCGACGCTGCATGCGACCAACGCCAATCAGGCGCTGGATCGAATC
ATCAATATGTTTCCCGAGGAGAAGCGCGAACAGCTTTTGCAGACATTGGGCAACAACCTCAAAGCCTGTGTTTCGCAGCG
TCTGGTCCGCACTGTCGATGGGCAAAGGCGCGCGGCAGTCGAGATTCTTCTGGGCACGCCGACCATCGCCGATCTGATCC
GCCGCGGTCAGTTCGAGGAACTCAAACCGATGATGGAAAAATCGACCGGACTCGGTATGCAGACATTTGACGCGGCACTG
TTTGCGCTGTTTGCAGAAGGTGCAATCAGTGAGCAGGAAGCATTGAAGAATGCCGATTCGGTGAATAACTTGAAGTTGCG
AATCAAACTTCAGCACGACCAGGCAGGTGTTAACAATACAGGCACCGGCGAGTGGGGTTTGATGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(58-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

55.182

96.748

0.534
  pilU Acinetobacter baylyi ADP1

53.409

95.393

0.509
  pilU Vibrio cholerae strain A1552

51.143

94.851

0.485
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.593

91.87

0.382
  pilT Pseudomonas stutzeri DSM 10701

39.826

93.225

0.371
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.541

90.244

0.366
  pilT Vibrio cholerae strain A1552

40.541

90.244

0.366
  pilT Pseudomonas aeruginosa PAK

39.244

93.225

0.366

Multiple sequence alignment