Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   ABW387_RS08970 Genome accession   NZ_CP159911
Coordinates   2053916..2055148 (-) Length   410 a.a.
NCBI ID   WP_025329863.1    Uniprot ID   -
Organism   Snodgrassella alvi strain Amel02     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2048916..2060148
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABW387_RS08935 (ABW387_01140) - 2049464..2049712 (-) 249 WP_025329856.1 BolA family protein -
  ABW387_RS08940 (ABW387_01135) - 2049767..2050528 (-) 762 WP_370385762.1 ABC transporter permease -
  ABW387_RS08945 (ABW387_01130) - 2050525..2051445 (-) 921 WP_370385763.1 ABC transporter ATP-binding protein -
  ABW387_RS08950 (ABW387_01125) yacG 2051492..2051689 (-) 198 WP_025329859.1 DNA gyrase inhibitor YacG -
  ABW387_RS08955 (ABW387_01120) zapD 2051689..2052441 (-) 753 WP_025329860.1 cell division protein ZapD -
  ABW387_RS08960 (ABW387_18330) coaE 2052447..2053061 (-) 615 WP_084551530.1 dephospho-CoA kinase -
  ABW387_RS08965 (ABW387_01105) pilD 2053058..2053909 (-) 852 WP_370385764.1 A24 family peptidase Machinery gene
  ABW387_RS08970 (ABW387_01100) pilG 2053916..2055148 (-) 1233 WP_025329863.1 type II secretion system F family protein Machinery gene
  ABW387_RS08975 (ABW387_01095) pilF 2055197..2056876 (-) 1680 WP_370385765.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABW387_RS08980 (ABW387_01090) recJ 2057078..2058775 (-) 1698 WP_370385766.1 single-stranded-DNA-specific exonuclease RecJ -
  ABW387_RS08985 (ABW387_01085) - 2058772..2059746 (-) 975 WP_367465533.1 hypothetical protein -

Sequence

Protein


Download         Length: 410 a.a.        Molecular weight: 45046.94 Da        Isoelectric Point: 9.8445

>NTDB_id=1016697 ABW387_RS08970 WP_025329863.1 2053916..2055148(-) (pilG) [Snodgrassella alvi strain Amel02]
MNKPVAVSKTKSSGTSFSFEGKNLKTEQIVRGEVVAKDEADARAKLTRRQIRVIQIAKVKKQRQKKISQADITVFTRQLS
TMMKSGLPMMQAFDIAAKGHSNPAMTQLLLQIRSDVEQGTSLAESFSKHPKYFDHFYCNLVHAGEAGGVLEAILDKLSVY
KEKTQGIKKKVKSALTYPAVVVVVAIALVIVMMMFVLPSFGKVYESMGVPLPGLTQFMMNISNFFVHYGIFVIIALIGGV
IGLFKWYQNSDDLKKRFDYMLLKMPIFGQIVRKATIARWSRTTATLFAAGVPLVEVLDSVAGASGNIRYEEATQEIRAKV
NQGISLTSAMQATELFPNMVMQMASIGEESGSLDDMLNKAAEFYEEDVDTAVAQLSSLMEPIIMVVLGGIVGIILVAMYL
PLFNIGNAVG

Nucleotide


Download         Length: 1233 bp        

>NTDB_id=1016697 ABW387_RS08970 WP_025329863.1 2053916..2055148(-) (pilG) [Snodgrassella alvi strain Amel02]
ATGAATAAACCCGTAGCAGTATCCAAAACCAAATCTTCCGGTACCAGTTTCAGTTTTGAAGGCAAAAACCTTAAAACTGA
ACAGATTGTACGCGGAGAAGTTGTCGCAAAAGACGAGGCTGATGCACGCGCCAAGCTAACCCGGCGTCAAATTCGTGTAA
TACAGATTGCCAAGGTCAAAAAACAGCGCCAAAAGAAAATCAGTCAGGCAGATATTACCGTCTTTACCCGACAATTATCC
ACCATGATGAAATCTGGTCTGCCTATGATGCAGGCTTTCGATATTGCAGCCAAAGGCCATTCTAACCCGGCAATGACCCA
GCTACTCTTGCAAATCCGCTCTGATGTTGAGCAAGGTACATCTCTGGCAGAGTCTTTTTCTAAACATCCGAAATATTTCG
ATCATTTTTACTGTAATCTGGTGCATGCGGGTGAAGCCGGTGGTGTACTAGAGGCTATTCTTGACAAGCTGTCGGTATAT
AAAGAAAAAACTCAGGGTATCAAGAAAAAAGTTAAAAGTGCCTTGACCTACCCAGCTGTGGTAGTAGTCGTAGCAATCGC
ACTGGTAATCGTCATGATGATGTTCGTATTACCTTCATTTGGTAAAGTTTACGAGAGTATGGGCGTACCATTACCTGGTT
TGACTCAGTTCATGATGAATATTTCCAATTTCTTTGTACATTACGGCATATTTGTCATCATTGCACTTATAGGTGGTGTA
ATTGGCTTATTTAAATGGTATCAGAACTCAGATGATCTGAAAAAACGTTTTGATTATATGCTGTTAAAAATGCCTATTTT
CGGCCAAATCGTTCGTAAAGCCACCATTGCACGCTGGTCTCGTACAACAGCAACTCTATTTGCAGCAGGCGTACCATTGG
TAGAAGTTCTTGATTCAGTTGCCGGAGCTTCCGGTAATATTCGCTATGAAGAAGCTACTCAGGAAATCCGTGCCAAAGTT
AATCAGGGTATATCTCTTACTTCTGCCATGCAGGCAACCGAATTATTTCCTAACATGGTAATGCAAATGGCTTCAATCGG
TGAAGAATCAGGTTCTTTGGATGACATGCTCAATAAGGCCGCAGAATTCTATGAAGAAGATGTAGATACTGCCGTTGCAC
AACTGTCTTCATTGATGGAACCGATTATTATGGTTGTACTTGGTGGTATTGTCGGGATAATTCTGGTTGCCATGTATTTA
CCGCTATTTAATATCGGTAATGCTGTAGGTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(75-198)

Type II secretion system (T2SS), protein F (T2SSF)

(279-401)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

64.885

95.854

0.622
  pilG Neisseria meningitidis 44/76-A

64.122

95.854

0.615
  pilC Legionella pneumophila strain ERS1305867

48.12

97.317

0.468
  pilC Acinetobacter baylyi ADP1

44.361

97.317

0.432
  pilC Acinetobacter baumannii D1279779

44.361

97.317

0.432
  pilC Pseudomonas stutzeri DSM 10701

41.855

97.317

0.407
  pilC Vibrio cholerae strain A1552

38.06

98.049

0.373

Multiple sequence alignment