Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ABW387_RS08975 Genome accession   NZ_CP159911
Coordinates   2055197..2056876 (-) Length   559 a.a.
NCBI ID   WP_370385765.1    Uniprot ID   -
Organism   Snodgrassella alvi strain Amel02     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2050197..2061876
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABW387_RS08945 (ABW387_01130) - 2050525..2051445 (-) 921 WP_370385763.1 ABC transporter ATP-binding protein -
  ABW387_RS08950 (ABW387_01125) yacG 2051492..2051689 (-) 198 WP_025329859.1 DNA gyrase inhibitor YacG -
  ABW387_RS08955 (ABW387_01120) zapD 2051689..2052441 (-) 753 WP_025329860.1 cell division protein ZapD -
  ABW387_RS08960 (ABW387_18330) coaE 2052447..2053061 (-) 615 WP_084551530.1 dephospho-CoA kinase -
  ABW387_RS08965 (ABW387_01105) pilD 2053058..2053909 (-) 852 WP_370385764.1 A24 family peptidase Machinery gene
  ABW387_RS08970 (ABW387_01100) pilG 2053916..2055148 (-) 1233 WP_025329863.1 type II secretion system F family protein Machinery gene
  ABW387_RS08975 (ABW387_01095) pilF 2055197..2056876 (-) 1680 WP_370385765.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABW387_RS08980 (ABW387_01090) recJ 2057078..2058775 (-) 1698 WP_370385766.1 single-stranded-DNA-specific exonuclease RecJ -
  ABW387_RS08985 (ABW387_01085) - 2058772..2059746 (-) 975 WP_367465533.1 hypothetical protein -
  ABW387_RS08990 (ABW387_01080) - 2059853..2060389 (-) 537 WP_174832879.1 tyrosine-protein phosphatase -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 61904.43 Da        Isoelectric Point: 5.7840

>NTDB_id=1016698 ABW387_RS08975 WP_370385765.1 2055197..2056876(-) (pilF) [Snodgrassella alvi strain Amel02]
MSLGLIRVLYKHQIIDAEKAEKLRQELEQKRPVIPALLQSGAINADKLASFLSGVFGIPYLNLEPYNQSLVPLDLINLKI
MQQYICVPLFKRGNRLFLAVSDPTSIQHYQKIATDAGCVLNLVIVRDSQLRQLLENLGQESTNLLEDMDSIGDSSAVENN
AMSADDEDGENGPIAKFVFKILGDALNSGASDIHFEFYEEMARVRFRVDGQLREVVRMPLNIRGQIASRIKVMAKLDISE
KRIPQDGRIQIQFRKGAKPIDFRVSTLPTLFGEKIVMRILNSDAATLNIEQLGFEPFQKELLLEAIHRPYGMVLVTGPTG
SGKTVSLYTCLNILNTDSVNISTAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKA
AQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVNLIMAQRLVRRLCTCKAPAERPPEFALKKAGFSDEDLAKEWTF
YRPVGCERCKGKGYKGRAGVYEVMPVSDEMQRIIMNNGNEVDISTQALKEGLVDLRHAGLLKVMEGITSLDEILASTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=1016698 ABW387_RS08975 WP_370385765.1 2055197..2056876(-) (pilF) [Snodgrassella alvi strain Amel02]
ATGAGTCTTGGACTGATCAGGGTTTTATATAAGCACCAGATTATTGACGCAGAAAAAGCAGAAAAACTGCGTCAGGAACT
GGAACAGAAACGTCCGGTTATACCGGCACTACTACAATCTGGAGCCATCAATGCTGATAAACTGGCCAGCTTTCTGTCCG
GTGTATTCGGTATTCCTTACCTGAATCTGGAGCCATATAATCAAAGTCTGGTACCGCTGGATTTAATTAATCTGAAAATT
ATGCAGCAATACATTTGCGTGCCGCTATTCAAGCGCGGTAATCGACTGTTTTTGGCTGTATCTGATCCTACATCAATTCA
GCATTACCAGAAAATTGCCACAGATGCCGGCTGCGTACTGAATCTGGTTATAGTCCGCGATAGTCAGTTACGCCAATTAC
TGGAAAATCTTGGTCAGGAATCCACCAACCTTCTGGAAGATATGGATTCAATTGGTGACTCCAGTGCTGTAGAAAATAAC
GCCATGTCTGCAGATGACGAAGATGGCGAAAATGGACCAATTGCAAAATTTGTTTTTAAAATACTAGGTGATGCATTAAA
CAGCGGCGCATCAGATATCCATTTTGAATTTTATGAAGAAATGGCACGAGTACGCTTTCGCGTTGACGGACAGTTGCGTG
AAGTAGTACGTATGCCACTGAATATTCGCGGCCAGATCGCTTCACGTATTAAAGTAATGGCCAAACTGGACATTTCAGAA
AAACGTATTCCTCAGGACGGACGTATTCAGATTCAGTTCCGTAAAGGTGCCAAGCCGATTGATTTCCGTGTCAGCACACT
GCCCACTCTGTTCGGGGAAAAAATTGTAATGCGTATCCTGAATTCGGATGCGGCTACTCTGAATATTGAACAACTTGGTT
TTGAGCCATTTCAGAAAGAATTACTGCTTGAGGCCATCCACCGGCCATACGGTATGGTGCTGGTTACCGGCCCTACTGGT
TCCGGTAAAACCGTATCACTGTATACCTGCCTTAATATTCTTAATACCGACAGTGTTAACATTTCCACTGCTGAAGACCC
TGCGGAAATTAACCTGCCTGGTATTAATCAGGTAAATGTTAATGATAAACAAGGACTGACATTTGCTGCTGCGTTAAAAT
CATTTCTGCGCCAGGATCCGGATATCATTATGGTGGGGGAAATCCGTGATTTGGAAACCGCAGATATTGCCATTAAAGCT
GCACAAACCGGCCATATGGTATTTTCAACTTTGCATACCAATAATGCCCCTGCTACATTATCCCGTATGCTGAATATGGG
CGTTGCTCCGTTTAATATTGCCAGCTCAGTAAATCTGATTATGGCACAGCGTCTGGTGCGGCGTTTATGTACCTGCAAGG
CACCGGCAGAGCGTCCGCCTGAATTTGCACTGAAAAAAGCAGGTTTTTCAGATGAAGATCTCGCCAAAGAATGGACATTT
TATCGGCCGGTCGGCTGTGAAAGGTGTAAGGGCAAAGGCTATAAAGGACGTGCCGGCGTATATGAAGTTATGCCGGTCAG
TGATGAAATGCAACGTATTATCATGAATAATGGTAATGAAGTAGATATCAGCACTCAGGCGCTCAAGGAAGGTCTGGTCG
ATTTACGCCATGCCGGTTTACTGAAAGTGATGGAAGGCATTACGTCTCTGGACGAGATTCTTGCAAGCACTAATGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(180-448)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

67.857

100

0.68
  pilB Acinetobacter baylyi ADP1

51.601

100

0.519
  pilB Acinetobacter baumannii D1279779

52.741

94.633

0.499
  pilB Legionella pneumophila strain ERS1305867

49.033

100

0.499
  pilB Vibrio cholerae strain A1552

44.92

100

0.451
  pilB Vibrio campbellii strain DS40M4

44.405

100

0.447
  pilB Vibrio parahaemolyticus RIMD 2210633

43.816

100

0.444
  pilF Thermus thermophilus HB27

40.112

95.886

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.152

94.454

0.379

Multiple sequence alignment