Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABHF91_RS15305 Genome accession   NZ_CP158016
Coordinates   3245942..3247651 (+) Length   569 a.a.
NCBI ID   WP_409421110.1    Uniprot ID   -
Organism   Pseudaeromonas sp. ZJS20     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3240942..3252651
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABHF91_RS15280 (ABHF91_15265) ampD 3241169..3241732 (-) 564 WP_409421105.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ABHF91_RS15285 (ABHF91_15270) - 3242027..3242398 (+) 372 WP_409421106.1 endonuclease domain-containing protein -
  ABHF91_RS15290 (ABHF91_15275) - 3242545..3243707 (+) 1163 WP_409421107.1 IS3 family transposase -
  ABHF91_RS15295 (ABHF91_15280) nadC 3244007..3244858 (+) 852 WP_409421108.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ABHF91_RS15300 (ABHF91_15285) tapA 3245382..3245861 (+) 480 WP_409421109.1 type IVa pilus major pilin TapA -
  ABHF91_RS15305 (ABHF91_15290) pilB 3245942..3247651 (+) 1710 WP_409421110.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABHF91_RS15310 (ABHF91_15295) pilC 3247740..3248984 (+) 1245 WP_409421111.1 type II secretion system F family protein Machinery gene
  ABHF91_RS15315 (ABHF91_15300) pilD 3249400..3250269 (+) 870 WP_409421112.1 prepilin peptidase Machinery gene
  ABHF91_RS15320 (ABHF91_15305) coaE 3250273..3250932 (+) 660 WP_409421113.1 dephospho-CoA kinase -
  ABHF91_RS15325 (ABHF91_15310) zapD 3250965..3251699 (+) 735 WP_409421114.1 cell division protein ZapD -
  ABHF91_RS15330 (ABHF91_15315) yacG 3251714..3251914 (+) 201 WP_409421115.1 DNA gyrase inhibitor YacG -
  ABHF91_RS15335 (ABHF91_15320) mutT 3251911..3252321 (-) 411 WP_409421116.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62102.17 Da        Isoelectric Point: 5.1317

>NTDB_id=1009527 ABHF91_RS15305 WP_409421110.1 3245942..3247651(+) (pilB) [Pseudaeromonas sp. ZJS20]
MTASPNSGLALSLVASALLTDEDSQKYSAQARQQRKPLISFLVENKILGSAALAEFCEQEYGIPLLDLDAFDLTEIPQKY
LNHKLIDKHHALPIYTQGHTLYVAMSDPTNVTALEDFGFSFGLHTEALLVDELKLQQAIAKLTESESAALGVEELSEAEI
GELEVADDDGRMEDTAAGGGDDDAPIVKYINKVLLDAIRRGASDLHFEPYEHKYRIRFRVDGILHEIASPPVNLASRFSA
RLKVMSRLDIAERRLPQDGRIKLKLAKGKAIDLRVNTLPTLWGEKIVLRILDSSAAKLNIDILGFDERQKALYLGALDKP
QGMILVTGPTGSGKTVSLYTGLNILNTAERNISTAEDPVEINLPGINQVQVNPKAGLTFASALRAFLRQDPDVVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLLNMGVPAFNVASSVTLIMAQRLTRRLCEKCKQPEQIPEPELLALGY
SQDQIDEGIKLYKPVGCSECSGGYKGRVGIYEMMPMNEAIANLIMEGGNSLQISALAQEHGMRTLRQTALEKARLGVTSL
AEVNRVTNG

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=1009527 ABHF91_RS15305 WP_409421110.1 3245942..3247651(+) (pilB) [Pseudaeromonas sp. ZJS20]
ATGACAGCCTCTCCCAATAGCGGCTTGGCCCTTAGCCTGGTGGCCTCGGCCCTGCTCACCGACGAAGACTCCCAGAAGTA
CAGCGCCCAGGCCCGCCAGCAGCGCAAGCCCCTGATCAGCTTTCTGGTGGAAAACAAGATACTGGGCAGTGCCGCCCTGG
CGGAGTTTTGCGAGCAGGAGTATGGGATACCTCTGCTGGATCTTGATGCCTTCGATTTGACCGAGATCCCGCAGAAATAT
CTCAATCACAAGCTGATTGACAAACACCACGCCCTGCCCATCTACACCCAGGGCCACACCCTCTATGTGGCCATGTCCGA
CCCCACCAATGTCACCGCATTGGAAGACTTCGGCTTCAGCTTCGGCCTGCATACCGAGGCCCTGCTGGTGGACGAGCTGA
AACTCCAGCAGGCCATAGCCAAGCTCACGGAAAGCGAATCCGCAGCCCTGGGGGTGGAGGAGCTTAGCGAGGCGGAGATT
GGCGAGCTGGAGGTGGCCGACGACGATGGCCGCATGGAGGATACTGCGGCAGGCGGCGGCGATGACGACGCCCCCATCGT
CAAATACATCAACAAGGTGCTGCTGGATGCCATCCGCCGCGGCGCCTCGGATCTGCACTTCGAACCCTACGAGCACAAGT
ACCGCATCCGTTTCCGGGTGGACGGCATACTGCACGAGATCGCCAGCCCGCCGGTGAACCTCGCCAGCCGCTTCTCCGCC
CGTCTCAAGGTCATGTCCCGCCTGGATATCGCAGAGCGCCGCCTGCCCCAGGATGGCCGCATCAAGCTGAAACTGGCCAA
GGGCAAGGCCATCGATCTGCGGGTCAACACATTGCCCACCCTGTGGGGCGAGAAGATAGTGCTGCGTATCCTGGACTCCT
CCGCCGCCAAGCTCAACATCGACATCCTGGGCTTCGACGAGCGACAGAAGGCCCTCTATCTGGGGGCGCTGGACAAGCCC
CAGGGCATGATACTGGTGACGGGCCCCACCGGCTCAGGGAAAACCGTCTCCCTCTACACCGGCCTCAATATTCTCAACAC
CGCCGAGCGCAACATCTCCACCGCCGAAGACCCGGTGGAAATCAACCTGCCGGGCATCAACCAGGTGCAGGTGAATCCGA
AAGCCGGGCTCACCTTTGCCAGCGCCCTGCGGGCCTTCCTGCGGCAAGATCCCGATGTGGTCATGGTGGGGGAAATCCGC
GATCTGGAAACCGCCGAGATCGCCATCAAGGCCGCCCAGACCGGTCACCTGGTGCTCTCCACCCTGCACACCAACTCGGC
AGCCGAGACCCTGACCCGCCTGCTCAACATGGGGGTGCCGGCCTTCAACGTGGCCTCCTCCGTGACCCTGATCATGGCCC
AGCGCCTGACCCGCCGCCTGTGCGAGAAGTGTAAGCAACCCGAGCAGATCCCTGAGCCGGAGCTGCTGGCCCTGGGCTAC
AGTCAGGATCAGATCGACGAGGGGATCAAGTTGTACAAGCCGGTGGGCTGCAGCGAATGCTCCGGCGGCTACAAGGGCCG
GGTAGGTATCTACGAGATGATGCCCATGAACGAGGCCATCGCCAACCTTATCATGGAGGGGGGCAACTCCTTGCAGATCT
CTGCTCTGGCTCAGGAGCATGGCATGCGCACCCTGCGCCAAACGGCGCTGGAGAAGGCCAGGCTAGGCGTCACCAGCCTG
GCGGAGGTGAACCGCGTCACCAACGGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.041

95.079

0.552
  pilB Acinetobacter baylyi ADP1

55.044

99.297

0.547
  pilB Legionella pneumophila strain ERS1305867

55.16

98.77

0.545
  pilB Vibrio cholerae strain A1552

57.467

92.97

0.534
  pilB Vibrio parahaemolyticus RIMD 2210633

52.743

99.297

0.524
  pilB Vibrio campbellii strain DS40M4

54.135

93.497

0.506
  pilF Neisseria gonorrhoeae MS11

47.518

99.121

0.471
  pilF Thermus thermophilus HB27

39.928

97.715

0.39
  pilB/pilB1 Synechocystis sp. PCC 6803

41.667

88.576

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.388

85.062

0.369

Multiple sequence alignment