Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABJ384_RS13380 Genome accession   NZ_CP157981
Coordinates   2770734..2772446 (+) Length   570 a.a.
NCBI ID   WP_349927893.1    Uniprot ID   A0AAU7SWK8
Organism   Acinetobacter sp. A1-4-2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2765734..2777446
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABJ384_RS13340 (ABJ384_13330) nusA 2766478..2767962 (-) 1485 WP_349927887.1 transcription termination factor NusA -
  ABJ384_RS13345 (ABJ384_13335) rimP 2768003..2768527 (-) 525 WP_086195210.1 ribosome maturation factor RimP -
  ABJ384_RS13370 (ABJ384_13360) secG 2769320..2769649 (-) 330 WP_349927889.1 preprotein translocase subunit SecG -
  ABJ384_RS13375 (ABJ384_13365) tpiA 2769663..2770454 (-) 792 WP_349927891.1 triose-phosphate isomerase -
  ABJ384_RS13380 (ABJ384_13370) pilB 2770734..2772446 (+) 1713 WP_349927893.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABJ384_RS13385 (ABJ384_13375) pilC 2772474..2773703 (+) 1230 WP_349927895.1 type II secretion system F family protein Machinery gene
  ABJ384_RS13390 (ABJ384_13380) pilD 2773721..2774584 (+) 864 WP_349927896.1 A24 family peptidase Machinery gene
  ABJ384_RS13395 (ABJ384_13385) coaE 2774601..2775191 (+) 591 WP_349927898.1 dephospho-CoA kinase -
  ABJ384_RS13400 (ABJ384_13390) - 2775211..2776128 (-) 918 WP_349927900.1 DMT family transporter -
  ABJ384_RS13405 (ABJ384_13395) rlmB 2776160..2776909 (-) 750 WP_086195202.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ABJ384_RS13410 (ABJ384_13400) - 2777051..2777374 (-) 324 WP_086195201.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63226.61 Da        Isoelectric Point: 4.9038

>NTDB_id=1009488 ABJ384_RS13380 WP_349927893.1 2770734..2772446(+) (pilB) [Acinetobacter sp. A1-4-2]
MAALQGSPRFTGFIRCLVEDGLISAENMQSAILNAKKANQDIVPFLIDQLKISSLAIAEKISQEFGEPLFDLSAYDSAQI
IREGLDEKIISKHCILPIFKRGNLLYVATSNPTNIEAMDAIRFSSKMNIEAIIVEHQKLHQLIEQNFTQADSFEFSDEDI
DLNLEQDDLSQKEDEDAGPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRIDGVLRQIANPPLQLSTRLAS
RLKVMSQMDISEKRLPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPEQKELFMQALEKP
QGMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNAKVGLTFSAALKSFLRQDPDIVMVGEIR
DLETAEIAVKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPQCKIPAEIPGPSLLEMGF
TEEDLKHPDFQVYQPVGCSECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKSGFNNLRRSGLIKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=1009488 ABJ384_RS13380 WP_349927893.1 2770734..2772446(+) (pilB) [Acinetobacter sp. A1-4-2]
ATGGCAGCATTACAAGGTTCACCAAGATTTACGGGTTTTATTCGATGTTTAGTCGAAGATGGCTTAATTTCTGCTGAAAA
TATGCAGAGTGCAATATTGAATGCAAAAAAAGCCAATCAAGATATTGTGCCTTTTTTAATTGATCAGTTGAAAATCTCCT
CCCTGGCTATTGCAGAAAAAATTTCTCAAGAATTTGGCGAACCTCTTTTTGATTTGAGTGCCTATGACTCTGCTCAAATT
ATCCGAGAAGGGCTGGATGAAAAAATCATTAGCAAACATTGCATTCTACCCATTTTCAAACGCGGGAATTTACTCTACGT
CGCAACCAGCAATCCCACCAATATAGAAGCCATGGATGCGATCCGCTTCAGCAGTAAGATGAATATCGAAGCGATTATTG
TAGAGCACCAGAAATTACACCAGCTGATTGAACAAAACTTTACCCAGGCGGACAGTTTTGAGTTTTCTGATGAAGATATC
GATCTAAATCTAGAGCAAGATGATTTAAGTCAAAAAGAAGATGAAGATGCTGGTCCTCAAGGGGATGAAGCTCCCATCGT
TAAGTACATCAATAAACTGTTAATTGATGCCATTCGTATGGGTGCTTCGGATTTACATTTCGAACCGTATGAAAAGTCAT
ATCGGGTGCGTTATCGTATTGATGGCGTGCTCCGTCAAATTGCCAATCCGCCGTTACAGCTGTCCACACGTTTAGCCTCA
CGTTTAAAAGTCATGTCACAAATGGATATTTCTGAAAAGCGTTTACCTCAAGATGGACGGATCAAGCTCAAACTTTCAAA
AAGCAAAGCTATTGACTTTCGTGTCAACTCTTTACCTACCCTGTTTGGTGAAAAGCTGGTCCTGCGTATTCTGGATCCAT
CCAGTGCCATGCTGGGTATTGATGCATTGGGTTATGAACCAGAGCAAAAAGAACTGTTTATGCAAGCGCTGGAAAAACCT
CAAGGCATGCTACTGATTACAGGTCCAACCGGTTCCGGTAAAACCGTATCGCTTTATACGGGTTTGAATATTCTGAATCG
TGAAGATACCAATATTTCCACAGCTGAAGACCCAGTAGAAATTAACCTGGAAGGGATTAATCAGGTCAATGTTAACGCTA
AAGTCGGTTTAACCTTCTCTGCTGCGCTTAAATCATTTTTACGTCAAGATCCAGATATTGTCATGGTTGGTGAGATTCGA
GATCTGGAAACAGCTGAAATTGCCGTAAAAGCGGCCCAGACAGGTCATATGGTGATGTCAACCCTGCACACCAACAGTGC
ACCAGAAACCTTGACTCGTTTACGTAACATGGGGGTTCCTTCCTTTAACATTGCAACTTCTGTCAACCTAGTGATTGCAC
AACGTTTAGCACGTCGTCTATGTCCTCAGTGTAAAATTCCGGCAGAGATTCCTGGACCCAGCCTGCTGGAAATGGGTTTT
ACCGAGGAAGATCTTAAACATCCAGACTTTCAAGTATATCAACCGGTAGGCTGTTCCGAGTGCCGTGAAGGTTATAAAGG
TCGTGTCGGTATTTATGAGGTCATGAAAGTGACCCCTGAAATTTCGAAAATTATTATGGAAGATGGTAATGCACTTGAAA
TTGCAGCAGCATCTGAAAAATCGGGGTTTAATAATTTACGCCGTTCAGGATTAATTAAGGTTATGCAGGGGGTGACTTCC
CTTCAGGAAGTCAATCGTGTAACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-149)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80

100

0.8
  pilB Acinetobacter baylyi ADP1

76.14

100

0.761
  pilB Legionella pneumophila strain ERS1305867

52.886

94.211

0.498
  pilB Vibrio cholerae strain A1552

48.858

99.825

0.488
  pilF Neisseria gonorrhoeae MS11

49.291

98.947

0.488
  pilB Vibrio parahaemolyticus RIMD 2210633

48.929

98.246

0.481
  pilB Vibrio campbellii strain DS40M4

47.183

99.649

0.47
  pilB/pilB1 Synechocystis sp. PCC 6803

34.087

100

0.37
  pilF Thermus thermophilus HB27

36.861

99.474

0.367

Multiple sequence alignment