Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABNP46_RS02330 Genome accession   NZ_CP157875
Coordinates   477059..478765 (-) Length   568 a.a.
NCBI ID   WP_349920823.1    Uniprot ID   -
Organism   Aeromonas veronii strain Ichip_C_3-7     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 472059..483765
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABNP46_RS02300 (ABNP46_02295) mutT 472650..473057 (+) 408 WP_349920817.1 8-oxo-dGTP diphosphatase MutT -
  ABNP46_RS02305 (ABNP46_02300) yacG 473140..473334 (-) 195 WP_349920818.1 DNA gyrase inhibitor YacG -
  ABNP46_RS02310 (ABNP46_02305) zapD 473347..474069 (-) 723 WP_349920819.1 cell division protein ZapD -
  ABNP46_RS02315 (ABNP46_02310) coaE 474107..474721 (-) 615 WP_349920820.1 dephospho-CoA kinase -
  ABNP46_RS02320 (ABNP46_02315) pilD 474740..475612 (-) 873 WP_349920821.1 A24 family peptidase Machinery gene
  ABNP46_RS02325 (ABNP46_02320) pilC 475695..476936 (-) 1242 WP_349920822.1 type II secretion system F family protein Machinery gene
  ABNP46_RS02330 (ABNP46_02325) pilB 477059..478765 (-) 1707 WP_349920823.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  ABNP46_RS02335 (ABNP46_02330) - 478769..479209 (-) 441 WP_349922340.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ABNP46_RS02340 (ABNP46_02335) nadC 479539..480396 (-) 858 WP_349920824.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ABNP46_RS02345 (ABNP46_02340) - 480400..480870 (-) 471 WP_349920825.1 retropepsin-like aspartic protease -
  ABNP46_RS02350 (ABNP46_02345) ampD 481007..481582 (+) 576 WP_349920826.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ABNP46_RS02355 (ABNP46_02350) pdhR 481855..482619 (+) 765 WP_349920827.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62686.11 Da        Isoelectric Point: 5.5551

>NTDB_id=1008748 ABNP46_RS02330 WP_349920823.1 477059..478765(-) (pilB) [Aeromonas veronii strain Ichip_C_3-7]
MTSSPNSGLATSLAASSLLSEPDSQRYLSQARAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEESKLTTAIAKLMESEQDALGMDHIDEAEI
SELEVTDESSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKLSRSKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIDQLGFDDRQKAQYLNALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTLEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKASQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCEHCKAPEVIPENELLELGFS
QQQLIAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSDNIARLIMQGANSLQIAAIAQKEGMRNLRTSGLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=1008748 ABNP46_RS02330 WP_349920823.1 477059..478765(-) (pilB) [Aeromonas veronii strain Ichip_C_3-7]
ATGACCTCTAGCCCCAATAGCGGCCTGGCCACCAGCCTGGCCGCTTCTTCCCTCCTCAGTGAGCCAGACTCACAACGCTA
TCTGAGCCAGGCCAGGGCGCAGCGCAAACCCTTTGTGACCTTCCTGATTGAAAACGATATCCTCGACAGCAAGGCCCTCG
CCGATTTCTGTGAACTGGAATATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACTTGGCCGAGATCCCGCAGAAATAC
CTCAATCAGAAGCTGATTGAGAAGCACCATGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
TCCGACCAATGTGTCGGCGCTGGAGGATTTCGGATTCAGCTTCGGGCTGCACACCGAGGCCCTGCTGGTCGAAGAGAGCA
AGCTGACCACCGCCATCGCCAAGCTGATGGAGAGTGAGCAGGATGCCCTCGGCATGGATCATATCGATGAGGCCGAGATC
TCCGAGCTCGAAGTCACCGATGAGAGCTCGCGGCTCGACGAGAGCGTCAACACCGCGGATGACGATGCCCCCATCGTCAA
GTACATCAACAAGATCATGATGGATGCCATCAAGCGTGGCGCCTCGGATCTGCACTTCGAACCCTACGAAACCAAGTACC
GGATCCGCTTTCGCATCGACGGCATACTGCACGAGATTGCCACCCCGCCGGTCAATCTGGCCAATCGCTTCGCCGCCCGC
CTCAAGGTGATGGCGAGGCTCGATATCGCCGAGCGGCGGCTGCCCCAGGATGGCCGGATCAAGCTGAAACTGTCCCGCAG
CAAGTCCATGGACATGCGGGTCAACACCCTGCCGACCATGTGGGGCGAGAAGATCGTCATCCGCTTGCTGGACTCGTCGG
CGGCGCGCCTCAACATCGATCAACTCGGTTTCGACGACAGACAGAAAGCCCAATACCTGAACGCCCTCTCCAAGCCCCAG
GGTATGATCCTGGTGACCGGCCCCACCGGCTCGGGCAAGACGGTCTCCCTCTATACTGGCCTCAACATTCTCAACACGCT
GGAGGTCAATATCTCCACCGCCGAGGATCCGGTGGAGATCAACCTGCCGGGGGTCAATCAGGTGCAGATCAATCCCAAGG
CGGGTCTCACCTTCGCCAGCGCTCTGCGCTCCTTCCTGCGCCAGGATCCTGACATCGTCATGGTGGGGGAGATCCGGGAT
CTGGAAACCGCCGAGATAGCCATCAAGGCCTCCCAGACCGGCCACCTGGTGCTCTCGACCCTGCACACCAACTCGGCGGC
CGAGACGCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAATATCGCCTCCTCGGTGACCCTCATCATGGCCCAAC
GCCTGGCTCGCAAGCTGTGTGAACACTGCAAGGCGCCAGAGGTGATCCCCGAGAACGAGCTGCTGGAGCTCGGTTTCAGC
CAACAACAGTTGATCGCGGGGCTGCGGTTGTTCAAACCCGTGGGCTGCAAGGAGTGTTCGGGGGGTTACAGAGGGCGGGT
CGGCATCTATGAGATCATGCTGATGTCCGACAATATCGCCAGGCTCATCATGCAGGGGGCCAACTCCTTGCAGATTGCCG
CCATTGCCCAGAAAGAGGGGATGCGCAATCTGAGAACCTCGGGCCTTGAGAAGGCGCGGTTGGGCGTCACCAGCCTGGCT
GAAATAAACAGAATAACCACAAACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.203

99.824

0.551
  pilB Acinetobacter baylyi ADP1

54.737

100

0.549
  pilB Legionella pneumophila strain ERS1305867

53.982

99.472

0.537
  pilB Vibrio cholerae strain A1552

52.539

100

0.528
  pilB Vibrio campbellii strain DS40M4

50.353

99.648

0.502
  pilB Vibrio parahaemolyticus RIMD 2210633

50.534

98.944

0.5
  pilF Neisseria gonorrhoeae MS11

45.922

99.296

0.456
  pilF Thermus thermophilus HB27

40.341

92.958

0.375
  pilB/pilB1 Synechocystis sp. PCC 6803

37.523

96.655

0.363
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.443

85.035

0.361

Multiple sequence alignment