Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   Q9L42_RS06735 Genome accession   NZ_CP157743
Coordinates   1380172..1381884 (-) Length   570 a.a.
NCBI ID   WP_305909196.1    Uniprot ID   A0AAU7NXX9
Organism   Methylomarinum sp. Ch1-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1375172..1386884
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q9L42_RS06705 (Q9L42_006705) trxC 1375789..1376223 (-) 435 WP_305910268.1 thioredoxin TrxC -
  Q9L42_RS06710 (Q9L42_006710) yacG 1376308..1376511 (-) 204 WP_349432395.1 DNA gyrase inhibitor YacG -
  Q9L42_RS06715 (Q9L42_006715) zapD 1376511..1377281 (-) 771 WP_305909199.1 cell division protein ZapD -
  Q9L42_RS06720 (Q9L42_006720) coaE 1377426..1378016 (-) 591 WP_305909198.1 dephospho-CoA kinase -
  Q9L42_RS06725 (Q9L42_006725) pilD 1378022..1378894 (-) 873 WP_305909197.1 A24 family peptidase Machinery gene
  Q9L42_RS06730 (Q9L42_006730) pilC 1378946..1380169 (-) 1224 WP_349432396.1 type II secretion system F family protein Machinery gene
  Q9L42_RS06735 (Q9L42_006735) pilB 1380172..1381884 (-) 1713 WP_305909196.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Q9L42_RS06740 (Q9L42_006740) - 1382027..1382821 (-) 795 WP_305909195.1 response regulator -
  Q9L42_RS06745 (Q9L42_006745) - 1382824..1384278 (-) 1455 WP_305909194.1 HDOD domain-containing protein -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63205.56 Da        Isoelectric Point: 5.4281

>NTDB_id=1008105 Q9L42_RS06735 WP_305909196.1 1380172..1381884(-) (pilB) [Methylomarinum sp. Ch1-1]
MSTVVSDLQFSGLTKCLIRKGLLTEESAQTAFQEADKQKISLISYLVNNQHLDSKTIASFASLEFGVPFFDLDAIDIRSL
PVKLVDEKLIRHHHALPLFKRGNRLFVATSDPTNFQALDEIKFHTRLNTENILVEEDKLGKAIEASLEAADTSMADLLDE
DLENIDISGGEQETPKEENTSDIDDAPIVRFVNKILLDSIKKGVSDIHLEPFEKTFRIRFRGDGILYEAASPPANIANRI
ISRIKVMSRMDIAERRVPQDGRIKMQLSKNRAIDFRVNTCPTLFGEKVVLRILDPTSAQLGIEKLGFEPAQQQLFLKAIH
RPYGMVLVTGPTGSGKTVSLYTGLNILNSMERNISTAEDPVEITVEGINQVNVNPKAGLTFAEALKAFLRQDPDIIMVGE
IRDLETASIAVKAAQTGHLVLSTLHTNDAPQTINRLMQMGIEPFNIVSAVNLIMAQRLARRLCEHCKQPADYPDNIYLDA
GFNKEELAGLQIYTHVGCEHCNGGYKGRVGIYQVMPISEAMRALILRGGNAMEMAELSLSEGINDLRASGLLKVKQGITT
LEEIDRVTKE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=1008105 Q9L42_RS06735 WP_305909196.1 1380172..1381884(-) (pilB) [Methylomarinum sp. Ch1-1]
ATGTCGACAGTAGTAAGTGATTTACAATTCAGCGGGTTGACCAAATGTCTGATAAGAAAAGGTCTGCTGACAGAAGAAAG
CGCCCAAACCGCATTTCAAGAAGCCGATAAGCAAAAAATCTCGTTAATCAGCTACCTGGTTAATAACCAGCATCTAGACA
GTAAAACGATTGCGTCCTTCGCCTCGCTGGAATTCGGTGTGCCGTTTTTCGATCTCGACGCCATAGACATCCGCTCGCTG
CCGGTCAAACTGGTCGACGAAAAACTGATCAGGCACCATCATGCCCTGCCGCTGTTCAAGCGCGGCAATCGTTTATTTGT
CGCCACCTCCGATCCGACCAATTTTCAGGCGCTGGACGAGATCAAATTCCACACCCGTCTCAACACCGAAAATATTCTGG
TCGAAGAAGATAAGTTAGGCAAGGCGATCGAGGCTTCGCTGGAAGCGGCCGACACCTCGATGGCGGACCTGCTCGATGAA
GACCTGGAAAACATCGACATCTCTGGCGGCGAACAAGAGACTCCGAAAGAAGAAAACACTTCCGATATCGATGACGCTCC
GATTGTCCGTTTCGTTAACAAGATTCTGCTCGATTCGATTAAAAAAGGCGTATCCGATATCCACCTGGAGCCGTTCGAGA
AGACTTTCCGCATCCGCTTCCGCGGCGACGGCATACTCTATGAAGCCGCCAGCCCGCCCGCCAATATCGCCAACCGGATT
ATTTCCCGCATCAAGGTCATGTCCAGAATGGACATCGCCGAGCGCCGCGTGCCTCAGGACGGACGCATCAAGATGCAATT
ATCCAAGAACCGCGCCATCGACTTTCGGGTCAATACGTGCCCTACCCTGTTCGGCGAGAAAGTGGTGCTGAGGATACTCG
ACCCGACCAGCGCCCAACTCGGCATCGAGAAACTGGGCTTTGAACCGGCGCAACAACAACTCTTTCTTAAAGCGATTCAT
CGGCCTTATGGCATGGTGCTGGTCACCGGGCCGACCGGCAGCGGGAAGACGGTGTCGCTCTACACCGGCCTGAATATATT
GAACAGTATGGAACGGAACATTTCCACCGCCGAAGACCCGGTGGAAATTACCGTAGAAGGCATCAATCAGGTCAACGTCA
ACCCGAAAGCCGGCCTGACCTTCGCCGAGGCGCTGAAGGCCTTTTTACGTCAAGACCCCGATATCATCATGGTCGGTGAG
ATTCGCGACCTGGAAACCGCCAGCATCGCCGTAAAAGCCGCGCAAACCGGCCACCTCGTATTATCGACCCTGCACACCAA
CGATGCGCCGCAAACGATCAACCGCCTGATGCAAATGGGCATAGAGCCTTTCAATATCGTCTCCGCGGTCAATTTAATCA
TGGCGCAAAGGCTGGCCCGGCGCCTATGCGAGCACTGCAAACAACCGGCCGACTATCCGGACAACATCTACTTGGACGCA
GGTTTCAATAAAGAAGAATTGGCCGGTCTACAAATTTATACCCATGTCGGCTGTGAGCATTGCAATGGCGGCTATAAAGG
CCGGGTCGGCATTTATCAGGTCATGCCGATCAGCGAAGCCATGCGAGCCCTCATCCTACGCGGCGGCAATGCGATGGAAA
TGGCCGAGCTGTCGTTAAGCGAGGGCATCAACGATCTGAGGGCCTCCGGTCTGCTCAAGGTCAAGCAAGGCATCACCACG
TTGGAAGAAATTGACAGGGTAACGAAGGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

55.594

100

0.558
  pilB Acinetobacter baumannii D1279779

54.45

100

0.547
  pilB Acinetobacter baylyi ADP1

53.403

100

0.537
  pilF Neisseria gonorrhoeae MS11

48.221

98.596

0.475
  pilB Vibrio parahaemolyticus RIMD 2210633

47.619

99.474

0.474
  pilB Vibrio campbellii strain DS40M4

47.257

99.123

0.468
  pilB Vibrio cholerae strain A1552

51.085

88.947

0.454
  pilF Thermus thermophilus HB27

39.177

98.07

0.384
  pilB/pilB1 Synechocystis sp. PCC 6803

41.477

92.632

0.384
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.23

99.123

0.379

Multiple sequence alignment