Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABL847_RS27235 Genome accession   NZ_CP157616
Coordinates   5834331..5836067 (-) Length   578 a.a.
NCBI ID   WP_077000884.1    Uniprot ID   -
Organism   Variovorax sp. KK3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5829331..5841067
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABL847_RS27215 (ABL847_27170) - 5829981..5831474 (-) 1494 WP_077000881.1 MBOAT family O-acyltransferase -
  ABL847_RS27220 (ABL847_27175) coaE 5831594..5832172 (-) 579 WP_077000916.1 dephospho-CoA kinase -
  ABL847_RS27225 (ABL847_27180) pilD 5832187..5833092 (-) 906 WP_077000882.1 prepilin peptidase Machinery gene
  ABL847_RS27230 (ABL847_27185) pilC 5833092..5834315 (-) 1224 WP_077000883.1 type II secretion system F family protein Machinery gene
  ABL847_RS27235 (ABL847_27190) pilB 5834331..5836067 (-) 1737 WP_077000884.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62714.94 Da        Isoelectric Point: 5.4510

>NTDB_id=1007734 ABL847_RS27235 WP_077000884.1 5834331..5836067(-) (pilB) [Variovorax sp. KK3]
MAAAEPLVKDSASIALPGLARALISAGKLPAKTAEEIYQKSTSGRTSFIAELTGTGAVSPADLAHTLSSAFGAPLLDLDA
IDAQRLPKDLLDPKLCQAYRVVVLSKRNNRLIVATADPSDQQAVEKIKFASQMGVDWVIAEYDKLSRMVEAAAVSASETI
DSIVGVEFEFDDSTIQDTPADSNDSASAEVEDAPVVRFLHKMLLDAFSMRASDIHFEPYEHQYRVRFRVDGELREVATPP
TVIKEKLASRIKVISRLDISEKRVPQDGRMKLKIGPDRVIDFRVSTLPTLFGEKIVVRILDPSSARMGIDALGYDTVEKE
RLLDAIGRPYGMVLVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINMPGVNQVNVNDKAGLTFATALRAFLRQDP
DIIMVGEIRDLETADIAIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPVCKAPSEVP
KEALIDAGYRDDEIDGTWKAYKPVGCPACNGGYKGRVGIYQVMPISEEIQKIILRDGSALEIAHQAESEGVRSLRRSGLH
KVMQGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=1007734 ABL847_RS27235 WP_077000884.1 5834331..5836067(-) (pilB) [Variovorax sp. KK3]
ATGGCCGCCGCTGAACCCCTCGTCAAAGATAGCGCTTCGATCGCCCTGCCCGGGCTCGCACGCGCCCTGATCTCCGCCGG
CAAGCTGCCCGCGAAGACGGCGGAGGAGATCTATCAGAAGTCGACCAGCGGCCGCACCAGCTTCATCGCGGAGTTGACCG
GCACGGGTGCCGTGTCGCCGGCCGACCTGGCCCACACCCTGTCGAGCGCCTTCGGCGCCCCGCTGCTCGACCTGGACGCG
ATCGACGCGCAGCGCCTGCCCAAGGACCTGCTCGACCCGAAGCTGTGCCAGGCCTACCGCGTGGTGGTGCTGAGCAAGCG
CAACAACCGCCTCATCGTCGCGACGGCCGATCCGTCGGACCAGCAGGCGGTCGAGAAGATCAAGTTCGCGTCCCAGATGG
GCGTGGACTGGGTGATCGCGGAGTACGACAAGCTCTCGCGCATGGTCGAGGCCGCGGCCGTGAGCGCCTCCGAGACCATC
GACAGCATCGTCGGCGTCGAGTTCGAGTTCGACGACTCCACGATCCAGGACACCCCCGCCGACTCGAACGACTCGGCCAG
CGCCGAGGTCGAAGACGCGCCGGTCGTGCGCTTCCTGCACAAGATGCTGCTCGACGCCTTCAGCATGCGGGCGTCGGACA
TCCACTTCGAGCCCTACGAGCACCAGTACCGGGTGCGCTTCCGCGTCGACGGCGAACTGCGCGAAGTCGCGACGCCACCC
ACCGTCATCAAGGAGAAGCTGGCTTCGCGGATCAAGGTGATCTCGCGGCTGGACATTTCCGAGAAGCGCGTGCCGCAAGA
CGGCCGAATGAAGCTCAAGATCGGCCCCGACCGGGTCATCGACTTCCGGGTCAGCACCCTGCCCACGCTCTTCGGCGAAA
AGATCGTCGTGCGTATCCTCGACCCCAGCAGCGCCCGCATGGGCATCGACGCGCTGGGCTACGACACCGTCGAGAAAGAG
CGGCTGCTCGACGCGATCGGCCGTCCCTACGGCATGGTGCTGGTGACGGGCCCGACGGGCTCGGGCAAGACCGTCTCGCT
CTATACCTGCCTGAACCTGCTGAACAAGCCCGGCGTCAACATCGCGACCGCCGAGGACCCCTCCGAAATCAACATGCCGG
GCGTGAACCAGGTCAACGTCAACGACAAGGCGGGCCTGACCTTCGCGACCGCGCTGCGCGCCTTCCTGCGGCAGGATCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTCGAAACCGCCGACATCGCGATCAAGGCCGCGCAGACCGGCCACCTGGT
GCTGTCGACCCTGCACACCAACGACGCCCCGACCACGTTGACGCGGATGCGCAACATGGGCATCGCGCCTTTCAACATCG
CGTCCAGCGTGATCCTCATCACCGCACAGCGGCTGGCGCGCCGGCTTTGCCCGGTGTGCAAGGCACCGTCCGAGGTTCCC
AAGGAAGCGCTGATCGACGCCGGCTACCGCGACGACGAGATCGACGGCACCTGGAAGGCCTACAAGCCGGTCGGCTGCCC
CGCCTGCAACGGCGGCTACAAGGGCCGCGTGGGCATCTACCAGGTGATGCCGATCTCTGAAGAGATCCAGAAGATCATCC
TGCGCGACGGCAGCGCCCTCGAGATCGCGCACCAGGCCGAGTCCGAGGGCGTGCGCTCGCTGCGCCGGTCGGGTCTGCAC
AAGGTCATGCAGGGGCTCACCTCGCTCGAGGAAGTGCTCGCGGTGACCAACGAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-152)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.049

98.27

0.531
  pilB Acinetobacter baumannii D1279779

53.819

97.405

0.524
  pilF Neisseria gonorrhoeae MS11

52.837

97.578

0.516
  pilB Legionella pneumophila strain ERS1305867

49.123

98.616

0.484
  pilB Vibrio cholerae strain A1552

46.346

97.059

0.45
  pilB Vibrio parahaemolyticus RIMD 2210633

46.263

97.232

0.45
  pilB Vibrio campbellii strain DS40M4

45.664

97.751

0.446
  pilF Thermus thermophilus HB27

39.261

98.27

0.386
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.437

94.983

0.384

Multiple sequence alignment