Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ABL847_RS17575 Genome accession   NZ_CP157616
Coordinates   3841720..3842235 (-) Length   171 a.a.
NCBI ID   WP_076999897.1    Uniprot ID   -
Organism   Variovorax sp. KK3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3836720..3847235
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABL847_RS17555 (ABL847_17530) - 3837366..3838475 (-) 1110 WP_083745406.1 DegT/DnrJ/EryC1/StrS family aminotransferase -
  ABL847_RS17560 (ABL847_17535) - 3838493..3839671 (-) 1179 WP_076999894.1 hypothetical protein -
  ABL847_RS17565 (ABL847_17540) - 3839668..3840429 (-) 762 WP_143684628.1 hypothetical protein -
  ABL847_RS17570 (ABL847_17545) - 3840449..3841621 (-) 1173 WP_083745407.1 glycosyltransferase family 2 protein -
  ABL847_RS17575 (ABL847_17550) pilA 3841720..3842235 (-) 516 WP_076999897.1 pilin Machinery gene
  ABL847_RS17580 (ABL847_17555) - 3842441..3843247 (-) 807 WP_076999898.1 MBL fold metallo-hydrolase -
  ABL847_RS17585 (ABL847_17560) - 3843455..3844105 (-) 651 WP_076999899.1 FHA domain-containing protein -
  ABL847_RS17590 (ABL847_17565) - 3844172..3844981 (-) 810 WP_076999900.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  ABL847_RS17595 (ABL847_17570) - 3845014..3845697 (-) 684 WP_076999901.1 TerC family protein -
  ABL847_RS17600 (ABL847_17575) sucD 3845787..3846680 (-) 894 WP_076999902.1 succinate--CoA ligase subunit alpha -

Sequence

Protein


Download         Length: 171 a.a.        Molecular weight: 17372.82 Da        Isoelectric Point: 9.8575

>NTDB_id=1007724 ABL847_RS17575 WP_076999897.1 3841720..3842235(-) (pilA) [Variovorax sp. KK3]
MNRRSIARNVQKGFTLIELMIVVAIIGILAAVALPAYQDYTIRAKVTDGISLAGSAKTAVAENAANGAPFASGWTSPAAT
RNVSAVEIAQGTGVITIRYQTEIDNGATLTLTPLNGSRAAGTALSGTASASTIPTAGSITWVCASAGSTVTNSAVVKGTI
KDKYVPAECRT

Nucleotide


Download         Length: 516 bp        

>NTDB_id=1007724 ABL847_RS17575 WP_076999897.1 3841720..3842235(-) (pilA) [Variovorax sp. KK3]
ATGAACCGTCGTTCCATCGCACGCAACGTGCAAAAGGGTTTCACACTTATCGAACTGATGATCGTTGTGGCGATCATTGG
TATCTTGGCGGCGGTGGCGCTGCCGGCCTACCAGGACTACACCATCCGCGCCAAGGTCACGGACGGCATCTCGCTGGCCG
GCAGTGCAAAGACCGCTGTTGCCGAGAACGCTGCGAACGGCGCTCCCTTCGCTTCCGGCTGGACTTCACCGGCAGCTACC
CGCAACGTCTCTGCCGTTGAGATTGCCCAAGGCACGGGCGTGATCACGATCAGGTATCAGACTGAAATCGACAACGGCGC
CACGCTGACCCTCACCCCTTTGAACGGTTCGCGCGCTGCGGGCACCGCTCTCTCTGGCACCGCTTCGGCATCGACGATTC
CCACTGCTGGTTCGATCACCTGGGTCTGCGCTTCGGCTGGCAGCACCGTGACTAACTCCGCTGTCGTCAAGGGCACCATC
AAGGACAAGTACGTGCCTGCCGAGTGCCGTACCTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(43-170)

Prokaryotic N-terminal methylation motif (N_methyl)

(8-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

49.153

100

0.509
  pilA2 Legionella pneumophila str. Paris

47.879

96.491

0.462
  pilA2 Legionella pneumophila strain ERS1305867

47.879

96.491

0.462
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

35.821

100

0.421
  pilE Neisseria gonorrhoeae strain FA1090

40

100

0.409
  comP Acinetobacter baylyi ADP1

38.983

100

0.404
  pilE Neisseria gonorrhoeae MS11

36.842

100

0.368

Multiple sequence alignment