Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ABL848_RS03845 Genome accession   NZ_CP157611
Coordinates   833736..834965 (+) Length   409 a.a.
NCBI ID   WP_093302396.1    Uniprot ID   -
Organism   Variovorax sp. NFACC28     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 828736..839965
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABL848_RS03815 (ABL848_03815) cgtA 828822..829898 (-) 1077 WP_093302390.1 Obg family GTPase CgtA -
  ABL848_RS03820 (ABL848_03820) rpmA 829970..830230 (-) 261 WP_093057448.1 50S ribosomal protein L27 -
  ABL848_RS03825 (ABL848_03825) rplU 830243..830554 (-) 312 WP_057592097.1 50S ribosomal protein L21 -
  ABL848_RS03830 (ABL848_03830) - 830739..831731 (+) 993 WP_093302393.1 polyprenyl synthetase family protein -
  ABL848_RS03840 (ABL848_03840) pilB 831976..833709 (+) 1734 WP_093198333.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABL848_RS03845 (ABL848_03845) pilC 833736..834965 (+) 1230 WP_093302396.1 type II secretion system F family protein Machinery gene
  ABL848_RS03850 (ABL848_03850) - 834965..835930 (+) 966 WP_093302399.1 prepilin peptidase -
  ABL848_RS03855 (ABL848_03855) coaE 835938..836549 (+) 612 WP_176947478.1 dephospho-CoA kinase -
  ABL848_RS03860 (ABL848_03860) - 836619..838112 (+) 1494 WP_093302402.1 MBOAT family O-acyltransferase -
  ABL848_RS03865 (ABL848_03865) - 838114..839148 (+) 1035 WP_093198342.1 hypothetical protein -
  ABL848_RS03870 (ABL848_03870) zapD 839179..839934 (+) 756 WP_093198345.1 cell division protein ZapD -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 44747.44 Da        Isoelectric Point: 9.5420

>NTDB_id=1007667 ABL848_RS03845 WP_093302396.1 833736..834965(+) (pilC) [Variovorax sp. NFACC28]
MATVASSRTQPTHKEFVFEWEGKDRNGKLVRGELRAAGENQVQAALRRQGVLASKIKKRRMRSGKAIKPKDIAIFTRQLA
TMMKAGVPLMQSFDIVGRGNANASVAKLLNDIRSDVETGTSLSAAFRKFPKYFDNLYCNLVEAGEAAGILEELLDRLATY
MEKTEAIKSKIKSALMYPISVIVVAFIVIAIIMIFVIPAFKEVFTSFGADLPAPTLIVISISEFFVSYWWLIFGGIGGGL
YFFMQAWKRNERVQRTMDRLLLRIPVFGALIEKSCVARWTRTLATMFAAGVPLVEALDSVGGASGNTVYGDATAKIQQEV
STGTSLTTAMTNVNLFPSMVIQMTAIGEESGSIDHMLGKAADFYESEVDDMVAGLSSLMEPIIIVFLGTIIGGIVVSMYL
PIFKLGQVV

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=1007667 ABL848_RS03845 WP_093302396.1 833736..834965(+) (pilC) [Variovorax sp. NFACC28]
ATGGCAACAGTGGCATCCTCCCGCACCCAGCCCACACACAAGGAATTTGTCTTCGAATGGGAAGGCAAGGACCGCAACGG
CAAGCTGGTAAGAGGCGAACTTCGTGCTGCCGGCGAAAACCAGGTGCAGGCCGCTCTGCGCCGCCAGGGCGTGCTCGCCT
CCAAGATCAAGAAGCGCCGCATGCGCTCGGGCAAGGCGATCAAGCCCAAGGACATCGCGATCTTCACGCGCCAGCTGGCG
ACCATGATGAAGGCCGGCGTGCCCCTGATGCAGTCCTTCGACATCGTCGGGCGCGGCAACGCGAACGCGAGCGTGGCCAA
GCTGCTCAACGACATCCGCAGCGACGTGGAAACCGGTACGTCGCTCTCGGCTGCCTTCCGCAAGTTTCCGAAGTACTTCG
ACAACCTCTACTGCAACCTGGTGGAGGCCGGTGAAGCGGCCGGTATTCTCGAAGAACTGCTGGACCGGCTCGCCACCTAC
ATGGAGAAGACCGAGGCGATCAAGTCCAAGATCAAGTCGGCCCTGATGTATCCGATCTCGGTGATCGTGGTGGCATTCAT
CGTGATCGCCATCATCATGATCTTCGTGATTCCCGCGTTCAAGGAGGTGTTCACGTCGTTCGGAGCCGACCTGCCTGCAC
CGACGCTGATCGTGATCAGCATCAGCGAGTTCTTCGTGTCCTATTGGTGGCTGATCTTCGGCGGCATCGGTGGCGGACTC
TACTTCTTCATGCAGGCCTGGAAGCGCAACGAGCGCGTGCAAAGGACCATGGACCGCCTCCTGCTGAGAATCCCGGTTTT
CGGAGCCCTGATCGAAAAATCCTGCGTGGCCCGCTGGACCCGTACGCTGGCCACCATGTTCGCCGCCGGTGTCCCCCTGG
TCGAAGCCCTCGACTCCGTGGGCGGCGCTTCCGGCAACACCGTGTACGGCGACGCCACCGCGAAGATCCAGCAGGAGGTC
TCCACCGGCACGAGCCTCACGACCGCCATGACCAACGTCAACCTGTTCCCCTCCATGGTGATCCAGATGACCGCCATCGG
CGAAGAGTCCGGCTCCATCGACCATATGCTGGGCAAGGCGGCCGACTTCTACGAGTCCGAAGTCGACGACATGGTCGCAG
GCCTCTCCAGTCTGATGGAGCCCATCATCATCGTGTTCCTCGGCACCATCATCGGCGGCATCGTGGTGTCGATGTACCTG
CCCATCTTCAAGCTCGGCCAGGTCGTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(75-198)

Type II secretion system (T2SS), protein F (T2SSF)

(279-401)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

54.094

98.533

0.533
  pilG Neisseria gonorrhoeae MS11

51.889

97.066

0.504
  pilG Neisseria meningitidis 44/76-A

51.637

97.066

0.501
  pilC Acinetobacter baylyi ADP1

51.005

97.311

0.496
  pilC Legionella pneumophila strain ERS1305867

50.505

96.822

0.489
  pilC Acinetobacter baumannii D1279779

47.932

100

0.482
  pilC Vibrio cholerae strain A1552

40.955

97.311

0.399
  pilC Thermus thermophilus HB27

37.709

100

0.386
  pilC Vibrio campbellii strain DS40M4

39

97.8

0.381

Multiple sequence alignment