Detailed information NTG1007193

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ABLU28_RS05370
• Genome accession: NZ_CP157495
• Coordinates: 1046083..1046730 (+)
• Length: 215 a.a.
• NCBI ID: WP_058223210.1
• Uniprot ID: A0AAP3Z0X8
• Organism: Lactococcus lactis strain 2B-1
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1041083..1051730

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABLU28_RS05345 (ABLU28_05345)	-	1041213..1042391 (+)	1179	WP_012898345.1	SLC13 family permease	-
ABLU28_RS05350 (ABLU28_05350)	-	1042564..1043127 (+)	564	WP_153241535.1	GNAT family N-acetyltransferase	-
ABLU28_RS05355 (ABLU28_05355)	-	1043231..1043629 (+)	399	WP_004255236.1	hypothetical protein	-
ABLU28_RS05360 (ABLU28_05360)	-	1043733..1044665 (+)	933	WP_406834733.1	ABC transporter ATP-binding protein	-
ABLU28_RS05365 (ABLU28_05365)	-	1044662..1046023 (+)	1362	WP_029344292.1	ABC transporter permease	-
ABLU28_RS05370 (ABLU28_05370)	comEA	1046083..1046730 (+)	648	WP_058223210.1	ComEA family DNA-binding protein	Machinery gene
ABLU28_RS05375 (ABLU28_05375)	comEC	1046711..1048921 (+)	2211	WP_406834732.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ABLU28_RS05380 (ABLU28_05380)	-	1049206..1049982 (+)	777	WP_058220836.1	alpha/beta hydrolase family protein	-
ABLU28_RS05385 (ABLU28_05385)	-	1050167..1050382 (+)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
ABLU28_RS05390 (ABLU28_05390)	atpB	1050427..1051140 (+)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
ABLU28_RS05395 (ABLU28_05395)	atpF	1051155..1051661 (+)	507	WP_406840986.1	F0F1 ATP synthase subunit B	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23610.74 Da
• Isoelectric Point: 4.8659

>NTDB_id=1007193 ABLU28_RS05370 WP_058223210.1 1046083..1046730(+) (comEA) [Lactococcus lactis strain 2B-1]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
VTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=1007193 ABLU28_RS05370 WP_058223210.1 1046083..1046730(+) (comEA) [Lactococcus lactis strain 2B-1]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATCTAATTAAGGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
GTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATTGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (151-212)

• SLBB domain (SLBB) (70-125)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	75.688	100	0.767
comEA/celA/cilE	Streptococcus mitis SK321	44.444	100	0.447
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.119	100	0.437
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.096	100	0.419
comEA/celA/cilE	Streptococcus pneumoniae D39	41.096	100	0.419
comEA/celA/cilE	Streptococcus pneumoniae R6	41.096	100	0.419
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.284	100	0.419
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386
comEA	Staphylococcus aureus MW2	37.559	99.07	0.372