Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABLB98_RS01560 Genome accession   NZ_CP157435
Coordinates   325923..327638 (-) Length   571 a.a.
NCBI ID   WP_348883274.1    Uniprot ID   A0AB39KJ82
Organism   Acinetobacter sp. XH1639     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 320923..332638
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABLB98_RS01530 (ABLB98_01530) - 321002..321328 (+) 327 WP_004659291.1 pyrimidine/purine nucleoside phosphorylase -
  ABLB98_RS01535 (ABLB98_01535) rlmB 321511..322260 (+) 750 WP_348883277.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ABLB98_RS01540 (ABLB98_01540) - 322291..323202 (+) 912 WP_249570191.1 DMT family transporter -
  ABLB98_RS01545 (ABLB98_01545) coaE 323199..323804 (-) 606 WP_087543054.1 dephospho-CoA kinase -
  ABLB98_RS01550 (ABLB98_01550) pilD 323806..324666 (-) 861 WP_283772246.1 A24 family peptidase Machinery gene
  ABLB98_RS01555 (ABLB98_01555) pilC 324669..325898 (-) 1230 WP_348883276.1 type II secretion system F family protein Machinery gene
  ABLB98_RS01560 (ABLB98_01560) pilB 325923..327638 (-) 1716 WP_348883274.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABLB98_RS01565 (ABLB98_01565) tpiA 327982..328773 (+) 792 WP_348883273.1 triose-phosphate isomerase -
  ABLB98_RS01570 (ABLB98_01570) secG 328786..329115 (+) 330 WP_005162938.1 preprotein translocase subunit SecG -
  ABLB98_RS01590 (ABLB98_01590) rimP 329738..330262 (+) 525 WP_348883272.1 ribosome maturation factor RimP -
  ABLB98_RS01595 (ABLB98_01595) nusA 330300..331784 (+) 1485 WP_005218583.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63217.42 Da        Isoelectric Point: 5.1320

>NTDB_id=1006834 ABLB98_RS01560 WP_348883274.1 325923..327638(-) (pilB) [Acinetobacter sp. XH1639]
MSGLHTSSKFSGFIRRLVEDGHMTAANMQNAIDAAKKAKQDIVAHLIEQHRLSPLTIAESISVEFGEPLFDLAVYDVGLL
PRELTDNKLIQKHRVVPLTQRGQILYVATSNPTNIDALDAIRFNTKLNIEPIIVEHTKLEKLIEQHFGDSSSFDFGDSED
FDLDIDVNQDSPQEDDDASPQGDESPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIATPPLQMATRLA
SRLKVMSQMDISEKRMPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIEALGYEDDQKALFMEALEK
PQGMLLITGPTGSGKTVSLYTGLNILNTEDTNISTAEDPVEINLEGINQVNVNPKTGLTFSAALKAFLRQDPDVIMVGEI
RDLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKAPVDVPRQSLLELG
FTEQDLSNPDLQIFQPIGCAECREGYKGRVGIYEVMKVTPAISQIIMEDGNALQIAAASEKAGFDNLRRSGLKKVMQGIT
SLQEINRVTSE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=1006834 ABLB98_RS01560 WP_348883274.1 325923..327638(-) (pilB) [Acinetobacter sp. XH1639]
ATGTCAGGATTACATACGTCTTCAAAGTTTTCAGGGTTCATTCGACGTTTAGTTGAAGATGGTCATATGACTGCTGCAAA
TATGCAGAATGCAATTGATGCAGCCAAGAAAGCCAAACAAGATATTGTTGCTCATTTAATCGAACAACATCGTCTTTCTC
CACTTACCATTGCAGAAAGCATCTCTGTAGAATTTGGCGAACCACTTTTTGATTTGGCGGTTTACGATGTTGGATTATTA
CCTCGAGAATTAACCGACAATAAACTCATACAAAAACATCGTGTTGTTCCGTTAACACAACGTGGACAAATTTTGTATGT
TGCTACGAGCAATCCAACCAATATTGATGCATTAGATGCAATTCGTTTCAACACCAAACTTAATATTGAACCAATTATTG
TTGAGCATACTAAACTTGAAAAGTTAATCGAACAACATTTTGGTGATTCAAGTAGTTTCGATTTTGGTGACAGTGAGGAC
TTCGACTTAGATATTGATGTTAATCAAGATTCACCTCAAGAAGATGATGATGCATCACCGCAAGGTGACGAATCCCCAAT
TGTCAAATATATTAATAAGCTTTTAATTGATGCAATTCGCATGGGCGCTTCAGATTTACATTTTGAGCCCTATGAAAAAA
TGTACCGTGTGCGTTATCGTGTTGATGGTGTGCTCCGTCAAATCGCTACACCTCCTTTACAAATGGCAACCCGCTTAGCT
TCTCGTTTAAAAGTAATGTCTCAAATGGATATCTCTGAAAAACGAATGCCTCAAGATGGTCGTATTAAACTCAAATTGTC
AAAAACCAAAGCCATCGATTTCCGTGTAAACTCTTTACCCACACTGTTTGGGGAAAAATTAGTACTGCGTATTTTGGATC
CATCAAGCGCTATGCTCGGGATTGAAGCATTAGGCTATGAAGATGATCAGAAAGCATTATTCATGGAAGCACTTGAAAAG
CCTCAAGGAATGCTACTGATTACAGGTCCAACAGGTTCGGGTAAAACCGTATCGCTATATACTGGTCTAAATATTCTAAA
CACTGAAGATACCAATATTTCAACAGCAGAAGACCCTGTGGAAATTAACTTGGAAGGCATTAATCAGGTCAACGTGAATC
CAAAGACAGGATTGACTTTCTCTGCTGCATTAAAGGCTTTCTTACGCCAAGACCCTGATGTAATTATGGTCGGTGAGATT
CGTGATTTGGAAACAGCCGAAATCGCAATTAAAGCAGCGCAAACTGGTCATATGGTGATGTCAACACTGCATACCAACAG
CGCACCTGAAACTTTAACTCGTTTGCGTAATATGGGGGTACCCTCATTCAATATTGCAACCTCAGTCAATCTTGTCATTG
CACAACGTTTAGCACGACGCTTATGTTCACAATGTAAAGCACCCGTAGATGTACCTAGACAAAGTCTTTTAGAGTTAGGA
TTTACAGAACAGGATTTATCAAACCCTGATTTACAGATCTTTCAACCGATTGGCTGTGCGGAATGTCGAGAAGGGTACAA
AGGACGTGTAGGTATTTATGAGGTTATGAAAGTCACTCCTGCAATTTCTCAAATCATCATGGAAGATGGTAATGCACTAC
AAATTGCAGCGGCCTCAGAAAAAGCTGGTTTTGATAATCTGCGTCGTTCAGGTCTAAAAAAAGTGATGCAAGGTATTACT
TCTTTGCAAGAAATTAACCGAGTAACAAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)

Type II/IV secretion system protein (T2SSE)

(193-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

82.312

100

0.823
  pilB Acinetobacter baylyi ADP1

77.058

100

0.771
  pilB Legionella pneumophila strain ERS1305867

54.144

95.096

0.515
  pilF Neisseria gonorrhoeae MS11

48.174

100

0.485
  pilB Vibrio parahaemolyticus RIMD 2210633

49.286

98.074

0.483
  pilB Vibrio campbellii strain DS40M4

48.301

97.898

0.473
  pilB Vibrio cholerae strain A1552

51.257

90.543

0.464
  pilF Thermus thermophilus HB27

37.5

98.074

0.368
  pilB/pilB1 Synechocystis sp. PCC 6803

34.15

100

0.366
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.795

92.995

0.361

Multiple sequence alignment