Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABHN08_RS11365 Genome accession   NZ_CP157354
Coordinates   2499869..2500978 (-) Length   369 a.a.
NCBI ID   WP_016774502.1    Uniprot ID   A0AAQ2HZW3
Organism   Pseudomonas atacamensis strain GY-AP11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2494869..2505978
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABHN08_RS11360 (ABHN08_11360) - 2495976..2499734 (-) 3759 WP_348971469.1 hypothetical protein -
  ABHN08_RS11365 (ABHN08_11365) pilU 2499869..2500978 (-) 1110 WP_016774502.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABHN08_RS11370 (ABHN08_11370) - 2501088..2503637 (+) 2550 WP_024012751.1 PAS domain-containing protein -
  ABHN08_RS11375 (ABHN08_11375) - 2503678..2503953 (+) 276 WP_123442629.1 peptidylprolyl isomerase -
  ABHN08_RS11380 (ABHN08_11380) - 2504070..2504990 (-) 921 WP_125926822.1 sugar kinase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40841.77 Da        Isoelectric Point: 7.0374

>NTDB_id=1006205 ABHN08_RS11365 WP_016774502.1 2499869..2500978(-) (pilU) [Pseudomonas atacamensis strain GY-AP11]
MEIDALLSILSQKNGSDLFLSTGAAPSARIDGVLTALSDQPFKPGETAAIATSLMDAEQRREFDRDLEMNLAISRTGVGR
FRVNIFKQRNDVSIVIRNVKVDIPRFEDLKLPPVLLETVMLKRGLILFVGATSSGKSTSLAALIDHRNRHSAGHIVTIED
PIEYIHRHKRSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALSFADTGHLVLSTLHATNANQALDRI
INMFPEEKREQLLQTLGNNLKACVSQRLVRTVDGQRRAAVEILLGTPTIADLIRRGQFEELKPMMEKSTGLGMQTFDAAL
FALFAEGAISEQEALKNADSVNNLKLRIKLQHDQAGVNNTGTGEWGLMD

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=1006205 ABHN08_RS11365 WP_016774502.1 2499869..2500978(-) (pilU) [Pseudomonas atacamensis strain GY-AP11]
ATGGAAATCGATGCACTGTTGTCAATATTGTCGCAAAAAAATGGCTCGGATCTGTTCCTCTCTACCGGCGCAGCGCCGAG
TGCGCGCATTGATGGCGTGCTGACGGCGCTCAGCGATCAGCCATTCAAACCCGGCGAAACCGCGGCCATCGCCACCTCCC
TGATGGACGCCGAGCAGCGCCGGGAGTTCGACCGGGATCTGGAAATGAACCTGGCAATCTCCCGAACGGGCGTCGGGCGG
TTCCGGGTGAACATTTTCAAGCAGCGCAACGACGTTTCGATCGTGATCCGCAACGTCAAGGTCGACATCCCGCGATTCGA
AGACCTGAAGCTGCCGCCGGTGTTGCTGGAAACGGTGATGCTCAAACGTGGCCTGATTCTTTTTGTCGGCGCGACCAGTT
CAGGCAAGTCGACTTCACTGGCGGCACTCATCGATCACCGCAACCGTCACAGTGCCGGACACATTGTGACGATCGAAGAC
CCGATCGAATATATCCATCGGCACAAGCGCTCGATCATCAATCAGCGTGAAGTCGGCGTAGATACCCGAAGCTTTCATGC
GGCTTTGAAGAACACTCTGCGCCAGGCTCCGGATGTTGTATTGATTGGTGAGATCCGTGACCGCGAAACCATGGAGCATG
CACTGTCATTCGCTGATACCGGCCATCTTGTGTTGTCGACGCTGCATGCGACCAATGCCAATCAGGCACTGGATCGAATC
ATCAATATGTTTCCCGAGGAGAAGCGCGAACAGCTTTTGCAAACATTGGGCAACAACCTCAAAGCCTGTGTCTCGCAGCG
TCTGGTCCGCACTGTCGATGGGCAAAGGCGCGCGGCAGTCGAGATTCTTCTGGGCACGCCGACCATCGCCGATCTGATCC
GCCGCGGTCAGTTCGAGGAACTCAAACCGATGATGGAAAAATCGACCGGACTCGGTATGCAGACATTTGACGCGGCACTG
TTTGCGCTGTTTGCAGAAGGTGCAATCAGTGAGCAGGAAGCATTGAAGAATGCCGATTCGGTGAATAACTTGAAGTTGCG
AATCAAACTTCAGCACGACCAGGCAGGTGTTAACAATACAGGCACCGGCGAGTGGGGTTTGATGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(58-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

55.182

96.748

0.534
  pilU Acinetobacter baylyi ADP1

53.409

95.393

0.509
  pilU Vibrio cholerae strain A1552

51.143

94.851

0.485
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.593

91.87

0.382
  pilT Pseudomonas stutzeri DSM 10701

39.826

93.225

0.371
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.541

90.244

0.366
  pilT Vibrio cholerae strain A1552

40.541

90.244

0.366
  pilT Pseudomonas aeruginosa PAK

39.244

93.225

0.366

Multiple sequence alignment