Detailed information NTG0100329

Bioinformatically predicted

Overview

• Name: ssb
• Type: Machinery gene
• Locus tag: AACH30_RS09460
• Genome accession: NZ_AP027243
• Coordinates: 1923614..1924108 (-)
• Length: 164 a.a.
• NCBI ID: WP_003133131.1
• Uniprot ID: A0A1I4HNK8
• Organism: Lactococcus garvieae strain L21-68
• Function: ssDNA binding (predicted from homology) DNA processing

Genomic Context

Location: 1918614..1929108

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AACH30_RS09425 (LG21E68_18440)	recX	1918941..1919774 (+)	834	WP_265149790.1	recombination regulator RecX	-
AACH30_RS09430 (LG21E68_18450)	-	1919857..1920705 (+)	849	WP_265149791.1	MurR/RpiR family transcriptional regulator	-
AACH30_RS09435 (LG21E68_18460)	-	1920737..1921045 (+)	309	WP_014025499.1	DUF960 domain-containing protein	-
AACH30_RS09440 (LG21E68_18470)	-	1921079..1921846 (-)	768	WP_265149792.1	metallophosphoesterase	-
AACH30_RS09445 (LG21E68_18480)	-	1921931..1922212 (-)	282	WP_265149793.1	hypothetical protein	-
AACH30_RS09450 (LG21E68_18490)	-	1922275..1923138 (-)	864	WP_265149794.1	hypothetical protein	-
AACH30_RS09455 (LG21E68_18500)	rpsR	1923255..1923500 (-)	246	WP_003133134.1	30S ribosomal protein S18	-
AACH30_RS09460 (LG21E68_18510)	ssb	1923614..1924108 (-)	495	WP_003133131.1	single-stranded DNA-binding protein	Machinery gene
AACH30_RS09465 (LG21E68_18520)	rpsF	1924146..1924439 (-)	294	WP_003133129.1	30S ribosomal protein S6	-
AACH30_RS09470 (LG21E68_18530)	-	1924626..1924838 (-)	213	WP_016171080.1	hypothetical protein	-
AACH30_RS09475 (LG21E68_18540)	-	1925309..1926772 (+)	1464	WP_003133128.1	amino acid permease	-
AACH30_RS09480 (LG21E68_18550)	dnaX	1926805..1928463 (-)	1659	WP_265149797.1	DNA polymerase III subunit gamma/tau	-
AACH30_RS09485 (LG21E68_18560)	-	1928476..1929096 (-)	621	WP_265149799.1	phosphotransferase	-

Sequence

Protein

• Length: 164 a.a.
• Molecular weight: 18003.88 Da
• Isoelectric Point: 4.9533

>NTDB_id=100329 AACH30_RS09460 WP_003133131.1 1923614..1924108(-) (ssb) [Lactococcus garvieae strain L21-68]
MINNVVLVGRVVRDPELRYTPQNTAVATFTIAVNRRFKNAQGEREADFINCVIWRQSAENLANWAKKGALIGVTGSIQVR
NYENKEGQRVYVTEVLADNFQMLESRAAREGGSGSYSAPQQQQSQNRPFAGSNSSAGNATPDFGRDADPFGGSPMEISDD
DLPF

Nucleotide

• Length: 495 bp

>NTDB_id=100329 AACH30_RS09460 WP_003133131.1 1923614..1924108(-) (ssb) [Lactococcus garvieae strain L21-68]
ATGATTAATAATGTTGTTTTGGTCGGACGCGTTGTGCGTGACCCTGAACTGCGATATACACCGCAAAATACTGCGGTTGC
TACATTTACGATTGCTGTTAACCGTCGTTTTAAAAACGCGCAAGGCGAACGTGAAGCTGATTTCATTAACTGTGTCATCT
GGCGTCAATCTGCTGAAAACTTGGCAAATTGGGCTAAAAAAGGTGCCTTGATTGGTGTAACAGGTAGTATTCAAGTGCGA
AATTATGAGAACAAAGAAGGTCAACGTGTTTACGTGACTGAAGTATTGGCTGATAACTTCCAAATGCTTGAGAGTCGTGC
CGCACGTGAAGGTGGCTCTGGTTCATATTCTGCACCACAGCAACAACAATCACAAAATCGTCCTTTTGCTGGCTCTAACA
GCTCAGCAGGAAATGCAACACCTGATTTTGGTCGTGATGCAGATCCATTTGGTGGCTCACCAATGGAAATCTCAGATGAT
GATCTTCCATTCTAA

Domains

Predicted by InterproScan.

• Single-strand binding protein family (SSB) (2-103)

3D structure

• AlphaFold DB: A0A1I4HNK8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ssb	Latilactobacillus sakei subsp. sakei 23K	57.31	100	0.598
ssbA	Bacillus subtilis subsp. subtilis str. 168	53.933	100	0.585
ssbB/cilA	Streptococcus pneumoniae TIGR4	49.242	80.488	0.396
ssbB/cilA	Streptococcus pneumoniae D39	48.485	80.488	0.39
ssbB/cilA	Streptococcus pneumoniae R6	48.485	80.488	0.39
ssbB/cilA	Streptococcus mitis SK321	48.485	80.488	0.39
ssbB/cilA	Streptococcus mitis NCTC 12261	48.485	80.488	0.39
ssbB/cilA	Streptococcus pneumoniae Rx1	48.485	80.488	0.39
ssbB	Bacillus subtilis subsp. subtilis str. 168	57.547	64.634	0.372