Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABEF84_RS01485 Genome accession   NZ_CP156795
Coordinates   308367..310076 (-) Length   569 a.a.
NCBI ID   WP_347473740.1    Uniprot ID   -
Organism   Acinetobacter thermotolerans strain ANC 7912     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 303367..315076
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABEF84_RS01455 (ABEF84_01455) - 303423..303746 (+) 324 WP_034587531.1 pyrimidine/purine nucleoside phosphorylase -
  ABEF84_RS01460 (ABEF84_01460) rlmB 303899..304648 (+) 750 WP_034587529.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ABEF84_RS01465 (ABEF84_01465) - 304682..305602 (+) 921 WP_034587527.1 DMT family transporter -
  ABEF84_RS01470 (ABEF84_01470) coaE 305627..306226 (-) 600 WP_034587525.1 dephospho-CoA kinase -
  ABEF84_RS01475 (ABEF84_01475) pilD 306231..307088 (-) 858 WP_034587523.1 A24 family peptidase Machinery gene
  ABEF84_RS01480 (ABEF84_01480) pilC 307103..308335 (-) 1233 WP_034587521.1 type II secretion system F family protein Machinery gene
  ABEF84_RS01485 (ABEF84_01485) pilB 308367..310076 (-) 1710 WP_347473740.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABEF84_RS01490 (ABEF84_01490) tpiA 310359..311150 (+) 792 WP_347473741.1 triose-phosphate isomerase -
  ABEF84_RS01495 (ABEF84_01495) secG 311164..311493 (+) 330 WP_034587516.1 preprotein translocase subunit SecG -
  ABEF84_RS01525 (ABEF84_01525) rimP 312444..312968 (+) 525 WP_034587514.1 ribosome maturation factor RimP -
  ABEF84_RS01530 (ABEF84_01530) nusA 313004..314488 (+) 1485 WP_034587513.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63317.57 Da        Isoelectric Point: 4.7773

>NTDB_id=1002874 ABEF84_RS01485 WP_347473740.1 308367..310076(-) (pilB) [Acinetobacter thermotolerans strain ANC 7912]
MTAIQGSLRFTGFIRRLVDEGVISAPDMQSALAGAKQEKIDIVPYLIERHNISPTKIAETISTEFGEPLFDINAYDPALI
IREDFDEKIITKHRILPIFKSANILFVATSNPTNVEAIDAIRFATKLNIETIIVEYNKLEKLIEQNFAEESTFDFDDDFD
LDVGVDASDSNTEEEDTNTGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKIYRVRYRIDGVLRQVATPPLQLATRLSSR
LKVMSQMDISEKRVPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPDQKALFMEALDKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLQGINQVNVNPKVGLTFAAALKSFLRQDPDIVMVGEIRD
LETAEIAVKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKKLADIPKQSLLEMGFT
EEDLQNPEFQIYEPVGCNECREGYKGRVGIYEVMKVTPEISRIIMEDGNAIQIAEASARAGFNNLRRSGLLKVMQGVTSL
QEVNRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=1002874 ABEF84_RS01485 WP_347473740.1 308367..310076(-) (pilB) [Acinetobacter thermotolerans strain ANC 7912]
ATGACAGCTATTCAGGGATCTTTACGATTTACGGGGTTTATTCGTCGACTTGTCGATGAAGGGGTTATCTCTGCACCAGA
TATGCAGAGTGCTTTGGCCGGTGCAAAGCAAGAAAAAATCGACATCGTTCCATATCTGATTGAACGCCATAATATTTCAC
CAACTAAAATCGCTGAAACCATTTCGACTGAATTTGGCGAACCGCTGTTTGACATCAATGCCTACGATCCCGCCTTGATT
ATCCGTGAAGACTTTGATGAAAAGATCATCACCAAACATCGTATTTTGCCGATCTTTAAAAGTGCCAATATCCTGTTTGT
TGCGACCAGCAACCCGACCAATGTCGAAGCAATTGATGCGATCCGTTTTGCCACCAAGCTCAATATTGAAACAATTATTG
TCGAGTATAATAAACTCGAAAAACTGATCGAACAGAATTTTGCTGAAGAAAGTACCTTCGATTTTGATGATGATTTCGAC
CTTGATGTAGGTGTGGACGCGTCTGATTCAAATACTGAAGAGGAAGATACCAATACCGGTGATGAAGCACCGATTGTTAA
GTACATCAACAAGCTGCTCATTGATGCAATTCGGATGGGTGCATCAGACTTACACTTTGAACCGTATGAAAAAATATACC
GGGTACGCTACCGTATTGATGGTGTATTGCGACAAGTGGCAACACCACCGCTGCAACTCGCAACACGTCTTTCCTCTCGT
TTAAAAGTCATGTCACAGATGGACATTTCAGAAAAACGTGTTCCTCAGGATGGCCGTATCAAGTTAAAACTGTCCAAAAA
CAAGGCCATTGATTTCCGTGTTAACTCATTACCCACGTTGTTTGGTGAAAAAATTGTACTGCGTATCCTAGATCCGTCCA
GTGCCATGTTAGGTATTGATGCATTAGGTTATGAACCCGATCAAAAAGCCCTGTTTATGGAAGCCTTGGACAAACCACAG
GGTATGCTGCTGATCACGGGTCCGACTGGTTCTGGTAAAACCGTCTCCTTATATACCGGCCTGAATATTCTGAATCGTGA
AGATACCAATATTTCTACCGCCGAAGACCCAGTGGAAATCAACTTACAGGGCATTAACCAGGTGAACGTTAACCCGAAAG
TAGGATTAACCTTCGCTGCAGCACTGAAATCATTCCTGCGTCAAGATCCGGATATCGTCATGGTCGGTGAGATCCGAGAC
CTGGAAACAGCTGAAATTGCGGTTAAAGCAGCGCAGACTGGTCACATGGTGATGTCGACACTACACACCAACAGTGCTCC
AGAAACACTGACCCGTTTACGCAATATGGGTGTACCATCATTTAACATTGCCACCTCAGTCAATCTGGTGATTGCCCAGC
GTCTGGCGCGTCGTCTGTGTTCACAATGCAAAAAACTGGCTGATATTCCAAAGCAGAGTCTGCTGGAAATGGGCTTTACC
GAAGAAGATTTACAGAACCCGGAATTCCAGATTTATGAACCTGTAGGGTGCAATGAATGCCGTGAAGGTTATAAGGGCCG
TGTCGGCATTTATGAAGTCATGAAGGTGACACCGGAAATTTCACGCATTATTATGGAAGATGGCAACGCCATTCAGATTG
CCGAAGCTTCCGCACGCGCAGGTTTTAACAATTTACGCCGATCAGGGTTATTAAAGGTAATGCAGGGGGTGACTTCTCTT
CAGGAAGTCAATCGTGTTACCAGCGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

79.474

100

0.796
  pilB Acinetobacter baylyi ADP1

76.316

100

0.765
  pilB Legionella pneumophila strain ERS1305867

53.235

95.079

0.506
  pilB Vibrio cholerae strain A1552

49.204

99.297

0.489
  pilF Neisseria gonorrhoeae MS11

49.554

98.594

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

49.198

98.594

0.485
  pilB Vibrio campbellii strain DS40M4

48.282

97.188

0.469
  pilF Thermus thermophilus HB27

37.567

98.243

0.369
  pilB/pilB1 Synechocystis sp. PCC 6803

33.768

100

0.364

Multiple sequence alignment